A5035356657- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Redox biology and oxidative stress
- Monoclonal and Polyclonal Antibodies Research
- Antioxidant Activity and Oxidative Stress
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Biosensing Techniques and Applications
- Chemical Synthesis and Analysis
- Synthesis and Catalytic Reactions
- Biochemical and Molecular Research
- COVID-19 Clinical Research Studies
- Genomics, phytochemicals, and oxidative stress
- PI3K/AKT/mTOR signaling in cancer
- Microfluidic and Capillary Electrophoresis Applications
- Nitric Oxide and Endothelin Effects
- Cancer Mechanisms and Therapy
- Vitamin C and Antioxidants Research
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Microbial Natural Products and Biosynthesis
- DNA and Nucleic Acid Chemistry
- Receptor Mechanisms and Signaling
- Protein purification and stability
Aston University
2014-2025
University of Manchester
2020-2025
Royal Devon and Exeter Hospital
2023
Czech Academy of Sciences, Institute of Biotechnology
2020
Stanford University
2017
University of Exeter
2017
University of Glasgow
2004-2014
Glasgow Life
2014
University of Birmingham
2013
Kuwait University
2011
Lipidome profile of fluids and tissues is a growing field as the role lipids signaling molecules increasingly understood, relying on an effective representative extraction present. A number solvent systems suitable for lipid are commonly in use, though no comprehensive investigation their effectiveness across multiple classes has been carried out. To address this, human LDL from normolipidemic volunteers was used to evaluate five different protocols [Folch, Bligh Dyer, acidified methanol...
Analysis of ERK pathway circuitry suggests appropriate targets for inhibition, providing a guide drug development.
Urinary proteomics is emerging as a powerful non-invasive tool for diagnosis and monitoring of variety human diseases. We tested whether signatures urinary polypeptides can contribute to the existing biomarkers coronary artery disease (CAD). examined total 359 urine samples from 88 patients with severe CAD 282 controls. Spot was analyzed using capillary electrophoresis on-line coupled ESI-TOF-MS enabling characterization more than 1000 per sample. In first step "training set" biomarker...
The discovery of biomarkers is currently attracting much interest as it harbors great potential for the diagnosis and monitoring human diseases. Here we have used two advanced mass spectroscopy based technologies, surface enhanced laser desorption ionization (SELDI-MS) capillary electrophoresis/mass spectrometry (CE/MS), to obtain proteomic patterns urine samples from patients suffering membranous glomerulonephritis (MGN) healthy volunteers. results indicate that CE/MS analysis able display...
Ultrasonics offers the possibility of developing sophisticated fluid manipulation tools in lab-on-a-chip technologies. Here we demonstrate ability to shape ultrasonic fields by using phononic lattices, patterned on a disposable chip, carry out complex sequence fluidic manipulations required detect rodent malaria parasite Plasmodium berghei blood. To illustrate different that are available us, used acoustic produce rotational vortices mechanically lyse both red blood cells and parasitic...
Abstract Purpose : Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study the development well‐characterized “real life” sample that can be used reference standard in urine clinical studies. Experimental design We report on generation male and female samples are extensively characterized by different platforms methods (CE‐MS, LC‐MS, LC‐MS/MS, 1‐D gel analysis combination with nano‐LC MS/MS (using LTQ‐FT ultra), 2‐DE‐MS) their proteome peptidome. In...
Abstract Raf kinase inhibitory protein (RKIP) is a physiologic inhibitor of c-RAF and nuclear factor κB signaling that represses tumor invasion metastasis. Glycogen synthase kinase-3β (GSK3β) suppresses progression by downregulating multiple oncogenic pathways including Wnt cyclin D1 activation. Here, we show RKIP binds GSK3 proteins maintains GSK3β levels its active form. Depletion augments oxidative stress–mediated activation the p38 mitogen activated kinase, which, in turn, inactivates...
During clinical mastitis in dairy cows, the quantity of milk produced decreases and composition is altered. As severity inflammation associated with disease increases, chemical approaches that blood as a consequence increased permeability mammary barrier, or de novo intramammary synthesis, has been suggested for serum amyloid A3. A better understanding these events may provide new diagnosis treatment mastitis. The objective this study was to document changes protein during using proteomic...
Myeloperoxidase (MPO), an abundant enzyme in phagocytes, has been implicated the pathogenesis of various inflammatory diseases including atherosclerosis. The major oxidant produced by MPO, hypochlorous acid (HOCl), is able to modify a great variety biomolecules chlorination and/or oxidation. In this paper reactions lipids (preferentially unsaturated fatty acids and cholesterol) with either reagent HOCl or generated MPO-hydrogen peroxide-chloride system are reviewed. One issues whether...
Abstract Biological characterisation of membrane proteins lags behind that soluble proteins. This reflects issues with the traditional use detergents for extraction, as surrounding lipids are generally lost, adverse structural and functional consequences. In contrast, styrene maleic acid (SMA) copolymers offer a detergent-free method biological solubilisation to produce SMA-lipid particles (SMALPs) containing together their lipid environment. We report development reverse-phase LC-MS/MS...
This study compared the molecular lipidomic profile of LDL in patients with nondiabetic advanced renal disease and no evidence CVD to that age-matched controls, hypothesis it would reveal proatherogenic lipid alterations. was isolated from 10 normocholesterolemic stage 4/5 lipids were analyzed by accurate mass LC/MS. Top-down lipidomics analysis manual examination data identified 352 species, automated comparative demonstrated alterations disease. The total cholesterol content unchanged, but...
A dry matrix application for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used to profile the distribution of 4-bromophenyl-1,4-diazabicyclo(3.2.2)nonane-4-carboxylate, monohydrochloride (BDNC, SSR180711) in rat brain tissue sections. Matrix involved applying layers finely ground alpha-cyano-4-hydroxycinnamic acid (CHCA) surface sections thaw mounted onto MALDI targets. It not possible detect drug when a standard aqueous-organic solvent solution. The...
We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly room temperature for short medium periods time. Sections from mouse brain were cut in a cryostat microtome, placed on MALDI target allowed warm 30 s 3 h. then refrozen, fixed by ethanol treatment analysed MALDI-MSI. The intensity range markers seen vary across course, both increasing decreasing, with some changing significantly within also showed...