A5009381464- Gut microbiota and health
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- Genomics and Phylogenetic Studies
- Dysphagia Assessment and Management
- Parkinson's Disease Mechanisms and Treatments
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Zoonotic diseases and public health
- Probiotics and Fermented Foods
Predictive Biology (United States)
2023
Abstract Several studies have documented the significant impact of methodological choices in microbiome analyses. The myriad options available complicate replication results and generally limit comparability findings between independent that use differing techniques measurement pipelines. Here we describe Mosaic Standards Challenge (MSC), an international interlaboratory study designed to assess variables on results. MSC did not prescribe methods but rather asked participating labs analyze 7...
Long-read metagenomic sequencing is a powerful approach for cataloging the microbial diversity present in complex microbiomes, including human gut microbiome. We performed deep-sequencing experiment using PacBio HiFi reads to obtain metagenome-assembled genomes (MAGs) from pooled long-read metagenome assembly two methods (hifiasm-meta, metMDBG), used improved bioinformatic and proximity ligation binning strategies cluster contigs identify MAGs, developed novel framework compare consolidate...
Abstract Background Several studies have documented the significant impact of methodological choices in microbiome analyses. The myriad options available complicate replication results and generally limit comparability findings between independent that use differing techniques measurement pipelines. Here we describe Mosaic Standards Challenge (MSC), an international interlaboratory study designed to assess variables on results. MSC did not prescribe methods but rather asked participating...
(1) Background: This study evaluated the effects of BiotiQuest® Sugar Shift®, a novel probiotic formulation, for its impact on gut microbiome composition and metabolic health in type 2 diabetes mellitus (T2D). T2D is characterized by chronic inflammation imbalances, yet therapeutic potential targeted probiotics remains underexplored. (2) Methods: In 12-week randomized, double-blind, placebo-controlled trial, 64 adults with received either Shift or placebo capsules twice daily. Each dose...
Objective: The objective of this study is to assess the efficacy BiotiQuest™ Sugar Shift, a probiotic formulated transform monosaccharides and rehabilitate human gut microbiota, in stabilize HbA1c blood glucose, improve insulin resistance, reduce inflammation.
Inferior quality of biological material compromises data, slows discovery, and wastes research funds. The gut microbiome plays a critical role in human health disease, yet little attention has been given to optimizing collection processing methods stool.We collected the entire bowel movement from 2 healthy volunteers: one examine stool sample heterogeneity test handling parameters. Sequencing bioinformatic analyses were used composition.The profile varied depending on where subsample was...
Abstract Background. Inferior quality of biological material compromises data, slows discovery, and wastes research funds. The gut microbiome plays a critical role in human health disease, yet little attention has been given to optimizing collection processing methods stool. Methods. We collected the entire bowel movement from 2 healthy volunteers: one examine stool sample heterogeneity test handling parameters. Sequencing bioinformatic analyses were used composition. Results. profile varied...
ABSTRACT Background The gut microbiome is altered in several neurologic disorders including Parkinson’s disease (PD). Objectives Profile the fecal metagenome PD for alterations microbial composition, taxon abundance, metabolic pathways, and gene products, their relationship with progression. Methods Shotgun metagenomic sequencing was conducted on 244 stool donors from two independent cohorts United States, individuals (n=48, n=47, respectively), environmental Household Controls (HC, n=29,...
Abstract Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by hyperglycemia, insulin resistance and inflammation. Probiotics have been claimed effective in the management of obesity type mellitus. BiotiQuest™ Sugar Shift symbiotic formulation rationally designed for endogenous conversion glucose fructose to support restoration human gut microbiota, modulation intestinal glucose, production anti-inflammatory metabolites. We report results 12-week, double blind,...