A5082861093- Gut microbiota and health
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Microbial Metabolites in Food Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- Oral microbiology and periodontitis research
- Infant Nutrition and Health
- Infant Health and Development
- Dental Health and Care Utilization
- Viral gastroenteritis research and epidemiology
- Tryptophan and brain disorders
- Bacterial Genetics and Biotechnology
- Gastrointestinal motility and disorders
- Child Nutrition and Water Access
- Food composition and properties
- Breastfeeding Practices and Influences
- Food Security and Health in Diverse Populations
- Phytoestrogen effects and research
- Neuroendocrine regulation and behavior
- Metabolomics and Mass Spectrometry Studies
- Stress Responses and Cortisol
- Bladder and Urothelial Cancer Treatments
- Diet, Metabolism, and Disease
- Dietary Effects on Health
University of North Carolina at Chapel Hill
2016-2025
University of North Carolina Health Care
2013-2024
North Carolina State University
2004-2021
University of Michigan
2021
Magyar Agrár- és Élettudományi Egyetem
2021
Tufts University
2021
Center for Digestive and Liver Diseases
2017
Institute of Cell Biology and Neurobiology
2015
Communities In Schools of Orange County
2015
APC Microbiome Institute
2015
Lactobacillus acidophilus NCFM is a probiotic bacterium that has been produced commercially since 1972. The complete genome 1,993,564 nt and devoid of plasmids. average GC content 34.71% with 1,864 predicted ORFs, which 72.5% were functionally classified. Nine phage-related integrases predicted, but no prophages found. However, three unique regions designated as potential autonomous units (PAUs) identified. These resemble structure bear characteristics both plasmids phages. Analysis the PAUs...
The human gut microbiota is increasingly recognized as a player in colorectal cancer (CRC). While particular imbalances the have been linked to adenomas and cancer, no specific bacterium has identified risk factor. Recent studies reported high abundance of Fusobacterium CRC subjects compared normal subjects, but this observation not for adenomas, precursors. We assessed species rectal mucosa with (n = 48) without 67). also confirmed previous reports on 10 tumor tissues 9 matching by...
Advancements in Next Generation Sequencing (NGS) technologies regarding throughput, read length and accuracy had a major impact on microbiome research by significantly improving 16S rRNA amplicon sequencing. As rapid improvements sequencing platforms new data analysis pipelines are introduced, it is essential to evaluate their capabilities specific applications. The aim of this study was assess whether the same project-specific biological conclusions composition could be reached using...
The human gut microbiota is becoming increasingly recognized as a key factor in homeostasis and disease. lack of physiologically relevant vitro models to investigate host-microbe interactions considered substantial bottleneck for research. Organoids represent an attractive model system because they are derived from primary tissues embody properties the native lumen; however, access organoid lumen experimental perturbation challenging. Here, we report development validation high-throughput...
The development of the infant intestinal microbiome in response to dietary and other exposures may shape long-term metabolic immune function. We examined differences community structure function between four feeding groups, exclusively breastfed infants before introduction solid foods (EBF), non-exclusively (non-EBF), EBF after (EBF+S), non-EBF (non-EBF+S), tested whether out-of-home daycare attendance was associated with relative abundance gut bacteria. Bacterial 16S rRNA amplicon...
Significance Approximately 75% of the global human population are lactose malabsorbers. In a previous clinical trial, it was shown that feeding high-purity galactooligosaccharide (>95% GOS) could improve symptoms lactose-intolerant subjects, attaining tolerance in majority subjects. To investigate mechanism, we examined microbiome subjects before and after GOS feeding. The results show significant shift responsive individuals, including lactose-fermenting microbes their stools. prebiotic...
Irinotecan treats a range of solid tumors, but its effectiveness is severely limited by gastrointestinal (GI) tract toxicity caused gut bacterial β-glucuronidase (GUS) enzymes. Targeted GUS inhibitors have been shown to partially alleviate irinotecan-induced GI damage and resultant diarrhea in mice. Here, we unravel the mechanistic basis for protection microbial using vivo models. We use vitro, fimo, models determine whether inhibition alters anticancer efficacy irinotecan. demonstrate that...
The lactic acid bacteria are a functionally related group of organisms known primarily for their bioprocessing roles in food and beverages. More recently, selected members the have been implicated number probiotic that impact general health well-being. Genomic analyses multiple bacteria, at genus, species, strain level, now elucidated many genetic features direct fermentative roles. This information is providing an important platform understanding core mechanisms control regulate bacterial...
The transport and catabolic machinery involved in carbohydrate utilization by Lactobacillus acidophilus was characterized genetically using whole-genome cDNA microarrays. Global transcriptional profiles were determined for growth on glucose, fructose, sucrose, lactose, galactose, trehalose, raffinose, fructooligosaccharides. Hybridizations carried out a round-robin design, microarray data analyzed with two-stage mixed model ANOVA. Differentially expressed genes visualized hierarchical...
The atypical antipsychotic risperidone (RSP) is often associated with weight gain and cardiometabolic side effects. mechanisms for these adverse events are poorly understood and, undoubtedly, multifactorial in etiology. In light of growing evidence implicating the gut microbiome host's energy regulation xenobiotic metabolism, we hypothesized that RSP treatment would be changes children adolescents. Thus, impact chronic (>12 months) short-term use on pediatric psychiatrically ill male...
This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain human origin and native species found commonly in gastrointestinal tracts neonates adults. The plasmid-free was 1,894,360 bp size predicted to encode 1,810 genes. GC content 35.3%, similar its closest relatives, L. johnsonii NCC 533 (34%) acidophilus NCFM (34%). Two identical copies prophage LgaI (40,086 bp), Sfi11-like Siphoviridae phage family, were integrated tandomly chromosome. A...
In silico genome analysis of Lactobacillus acidophilus NCFM coupled with gene expression studies have identified putative genes and regulatory networks that are potentially important to this organism's survival, persistence, activities in the gastrointestinal tract. Correlation key genotypes phenotypes requires an efficient replacement system. study, use upp-encoded uracil phosphoribosyltransferase (UPRTase) L. was explored as a counterselection marker positively select for recombinants...
ABSTRACT Intestinal microbial dysbiosis contributes to the dysmetabolism of luminal factors, including steroid hormones (sterones) that affect development chronic gastrointestinal inflammation and incidence sterone-responsive cancers breast, prostate, colon. Little is known, however, about role specific host sterone nucleoreceptors, estrogen receptor β (ERβ), in microbiota maintenance. Herein, we test hypothesis ERβ status affects composition determine if such compositionally distinct...
Abstract The purpose of this study is to evaluate similarities and differences in gut bacterial measurements stability the microbial communities three different types samples that could be used assess niches microbiome: rectal swab, stool, normal mucosa samples. In swab-stool comparisons, there were substantial taxa with some varying largely by sample type (e.g. Thermaceae), inter-individual subject variation Desulfovibrionaceae), or both participant Enterobacteriaceae). Comparing all...
Colorectal cancer (CRC) is the third most common in world and second leading cause of deaths US Spain. The molecular mechanisms involved etiology CRC are not yet elucidated due part to complexity human gut microbiota. In this study, we compared microbiome composition 90 tumor matching adjacent tissue (adjacent) from cohorts Spain by 16S rRNA amplicon sequencing order determine impact geographic origin on microbiome. Data showed a significantly (P < 0.05) higher Phylogenetic Diversity (PD)...
Prebiotics are ingredients selectively fermented by the intestinal microbiota that promote changes in its structure and/or metabolism, conferring health benefits to host. Studies show β (1-4) galacto-oligosaccharides [β GOS], lactulose and fructo-oligosaccharides increase concentration of lactate short chain fatty acids, stool frequency weight, they decrease fecal secondary bile pH, nitroreductase β-glucuronidase activities suggesting a clear role colorectal cancer (CRC) prevention. This...