A5001582402- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Infant Nutrition and Health
- Bacteriophages and microbial interactions
- Digestive system and related health
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Transgenic Plants and Applications
- Viral gastroenteritis research and epidemiology
- Advanced Proteomics Techniques and Applications
- Insect symbiosis and bacterial influences
- Food composition and properties
- Food Quality and Safety Studies
- Reproductive tract infections research
- Fungal Biology and Applications
- Protein Hydrolysis and Bioactive Peptides
- Animal Virus Infections Studies
- Inflammatory Bowel Disease
- Immune Response and Inflammation
North Carolina State University
2014-2023
AgBiome (United States)
2023
Research Triangle Park Foundation
2023
Intellia Therapeutics (United States)
2019
Suncheon Jeil College
2019
Digital Proteomics (United States)
2016
Nutrition Sciences (Belgium)
2009-2014
Norfolk State University
2012
University of Nebraska–Lincoln
2006-2011
Andong National University
2007
Lactic acid-producing bacteria are associated with various plant and animal niches play a key role in the production of fermented foods beverages. We report nine genome sequences representing phylogenetic functional diversity these bacteria. The small genomes lactic acid encode broad repertoire transporters for efficient carbon nitrogen acquisition from nutritionally rich environments they inhabit reflect limited range biosynthetic capabilities that indicate both prototrophic auxotrophic...
CRISPR-Cas systems are now widely used for genome editing and transcriptional regulation in diverse organisms. The compact portable nature of class 2 single effector nucleases, such as Cas9 or Cas12, has facilitated directed modifications plants, animals, microbes. However, most belong to the more prevalent 1 category, which hinges on multiprotein complexes. In present study, we detail how native type I-E system, with a 5′-AAA-3′ protospacer adjacent motif (PAM) 61-nucleotide guide CRISPR...
This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain human origin and native species found commonly in gastrointestinal tracts neonates adults. The plasmid-free was 1,894,360 bp size predicted to encode 1,810 genes. GC content 35.3%, similar its closest relatives, L. johnsonii NCC 533 (34%) acidophilus NCFM (34%). Two identical copies prophage LgaI (40,086 bp), Sfi11-like Siphoviridae phage family, were integrated tandomly chromosome. A...
In silico genome analysis of Lactobacillus acidophilus NCFM coupled with gene expression studies have identified putative genes and regulatory networks that are potentially important to this organism's survival, persistence, activities in the gastrointestinal tract. Correlation key genotypes phenotypes requires an efficient replacement system. study, use upp-encoded uracil phosphoribosyltransferase (UPRTase) L. was explored as a counterselection marker positively select for recombinants...
ABSTRACT Aggregation-promoting factors (Apf) are secreted proteins that have been associated with a diverse number of functional roles in lactobacilli, including self-aggregation, the bridging conjugal pairs, coaggregation other commensal or pathogenic bacteria, and maintenance cell shape. In silico genome analysis Lactobacillus acidophilus NCFM identified LBA0493 as 696-bp apf gene encodes putative 21-kDa Apf protein. Transcriptional studies during growth milk showed to be one most highly...
Bacterial surface (S-) layers are crystalline arrays of self-assembling, proteinaceous subunits called S-layer proteins (Slps), with molecular masses ranging from 40 to 200 kDa. The S-layer-forming bacterium Lactobacillus acidophilus NCFM expresses three major Slps: SlpA (46 kDa), SlpB (47 kDa) and SlpX (51 kDa). has a demonstrated role in adhesion Caco-2 intestinal epithelial cells vitro, been shown modulate dendritic cell (DC) T-cell functionalities murine DCs. In this study, modification...
Therapeutically active glycosylated phytochemicals are ubiquitous in the human diet. The gut microbiota (HGM) modulates bioactivities of these compounds, which consequently affect host physiology and composition. Despite a significant impact on health, key players underpinning mechanisms this interplay remain uncharacterized. Here, we demonstrate growth Lactobacillus acidophilus mono- diglucosyl dietary plant glycosides (PGs) possessing small aromatic aglycones. Transcriptional analysis...
Probiotic microbes rely on their ability to survive in the gastrointestinal tract, adhere mucosal surfaces, and metabolize available energy sources from dietary compounds, including prebiotics. Genome sequencing projects have proposed models for understanding prebiotic catabolism, but mechanisms remain be elucidated many substrates. Although β-galactooligosaccharides (GOS) are documented little is known about utilization by lactobacilli. This study aimed identify genetic loci Lactobacillus...
ABSTRACT The fosABCDXE operon encodes components of a putative fructose/mannose phosphoenolpyruvate-dependent phosphotransferase system and β-fructosidase precursor (FosE) that are involved in the fructooligosaccharide (FOS) utilization pathway Lactobacillus paracasei 1195. presence an N-terminal signal peptide sequence LPQAG cell wall anchor motif C-terminal region deduced FosE amino acid predicted enzyme is associated, indicating FOS may be hydrolyzed extracellularly. In this study,...
Streptococcus thermophilus represents the only species among streptococci that has "Generally Regarded As Safe" status and plays an economically important role in fermentation of yogurt cheeses. We conducted comparative genome analysis S. LMD-9 to identify unique gene features as well contribute its adaptation dairy environment. In addition, we investigated transcriptome response during growth milk presence Lactobacillus delbrueckii ssp. bulgaricus, a companion culture fermentation, lytic...
In prokaryotic species equipped with glycogen metabolism machinery, the co-regulation of biosynthesis and degradation has been associated synthesis energy storage compounds various crucial physiological functions, including global cellular processes such as carbon nitrogen metabolism, sensing production, stress response cell-cell communication. addition, metabolic pathway was proposed to serve a capacitor that regulates downstream fluxes, in some microorganisms ability synthesize...
Abstract Background Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential oligosaccharides were analyzed to identify genetic loci involved catabolism α- β-linked hexoses, β-xylosides....
Lactobacillus crispatus and gasseri are two of the main species found in healthy vaginal microbiome have also previously been identified isolated from human gastrointestinal (GI) tract. These ecological niches fundamentally different, notably with regards to epithelial cell type, nutrient availability, environmental conditions, pH, composition. Given dramatic differences between these environments, we characterized strains within same either or intestinal tract assess whether they...
Bifidobacterium is a commensal bacterial genus ubiquitous in the human gastrointestinal tract, which associated with range of health benefits. The advent CRISPR-based genome editing technologies provides opportunities to investigate genetics important bacteria and transcend lack genetic tools bifidobacteria study basis for their health-promoting attributes. Here, we repurpose endogenous type I-G CRISPR-Cas system adopt an exogenous CRISPR base editor engineering B. animalis subsp. lactis,...
The growth and activity of some Lactobacillus Bifidobacterium strains are stimulated by the presence nondigestible fructooligosaccharides (FOS), which selectively fermented specific intestinal bacteria. Consumption FOS, therefore, enriches for those bacteria that possess metabolic pathways necessary FOS metabolism. In this study, a DNA microarray consisting 7,680 random genomic library fragments paracasei 1195 was used to examine genes involved in utilization organism. Differential...
Glycogen metabolism contributes to energy storage and various physiological functions in some prokaryotes, including colonization persistence. A role for glycogen is proposed on the survival fitness of Lactobacillus acidophilus, a probiotic microbe, human gastrointestinal environment. L. acidophilus NCFM possesses (glg) operon consisting glgBCDAP-amy-pgm genes. Expression glg accumulation were carbon source- growth phase-dependent, repressed by glucose. The highest intracellular content was...
Surface proteins of probiotic microbes, including Lactobacillus acidophilus and gasseri, are believed to promote retention in the gut mediate host-bacterial communications. Sortase, an enzyme that covalently couples a subset extracellular containing LPXTG motif cell surface, is particular interest characterizing bacterial adherence communication with mucosal immune system. A sortase gene, srtA, was identified L. NCFM (LBA1244) gasseri ATCC 33323 (LGAS_0825). Additionally, eight six intact...
ABSTRACT CRISPR-Cas systems provide adaptive immunity against phages in prokaryotes via DNA-encoded, RNA-mediated, nuclease-dependent targeting and cleavage. Due to inefficient relatively limited DNA repair pathways bacteria, can be repurposed for lethal that selects sequence variants. In this study, the relative killing efficiencies of endogenous Type I II model organism Streptococcus thermophilus DGCC7710 were assessed. Additionally, genetic phenotypic outcomes chromosomal by...
This work describes the development of a lactobacillus CRISPR-based editing system for genome manipulations in three Lactobacillus species belonging to lactic acid bacteria (LAB), which are commonly known their long history use food fermentations and as indigenous members healthy microbiotas emerging roles human animal commercial health-promoting applications. We exploited established CRISPR-SpyCas9 nickase flexible precise applications acidophilus further demonstrated efficacy this...
Health-promoting aspects attributed to probiotic microorganisms, including adhesion intestinal epithelia and modulation of the host mucosal immune system, are mediated by proteins found on bacterial cell surface. Notably, certain commensal bacteria contain a surface (S-) layer as outermost stratum wall. S-layers non-covalently bound semi-porous, crystalline arrays self-assembling, proteinaceous subunits called S-layer (SLPs). Recent evidence has shown that multiple co-localized within...