A5005213966- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Viral Infections and Vectors
- Bacillus and Francisella bacterial research
- Probiotics and Fermented Foods
- Virus-based gene therapy research
- Immune Response and Inflammation
- Vector-borne infectious diseases
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Infant Nutrition and Health
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Veterinary Oncology Research
- Immune cells in cancer
- Herpesvirus Infections and Treatments
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Animal Disease Management and Epidemiology
- interferon and immune responses
- T-cell and B-cell Immunology
University of Florida
2015-2025
Florida College
2023-2025
Northwestern University
2022
Albany Medical Center Hospital
2006-2018
Office of Infectious Diseases
2017
Cancer Genetics (United States)
2012
Indian Veterinary Research Institute
2005-2009
Emerging evidence suggests that gut microbiota is critical in the maintenance of physiological homeostasis. This study was designed to test hypothesis dysbiosis associated with hypertension because genetic, environmental, and dietary factors profoundly influence both blood pressure. Bacterial DNA from fecal samples 2 rat models a small cohort patients used for bacterial genomic analysis. We observed significant decrease microbial richness, diversity, evenness spontaneously hypertensive rat,...
Phagocytosed Borrelia burgdorferi (Bb) induces inflammatory signals that differ both quantitatively and qualitatively from those generated by spirochetal lipoproteins interacting with Toll-like receptor (TLR) 1/2 on the surface of human monocytes. Of particular significance, in contrast to lipoproteins, internalized spirochetes induce transcription IFN-β. Using inhibitory immunoregulatory DNA sequences (IRSs) specific TLR7, TLR8, TLR9, we show TLR8 inhibitor IRS957 significantly diminishes...
The World health organization (WHO) declared Coronavirus disease 2019 (COVID-19) a global pandemic and severe public crisis. Drastic measures to combat COVID-19 are warranted due its contagiousness higher mortality rates, specifically in the aged patient population. At current stage, lack of effective treatment strategies for innovative approaches need be considered. It is well known that host cellular miRNAs can directly target both viral 3'UTR coding region genome induce antiviral effect....
The gut microflora is a vital component of the gastrointestinal (GI) system that regulates local and systemic immunity, inflammatory response, digestive system, overall health. Older people commonly suffer from inadequate nutrition or poor diets, which could potentially alter microbiota. essential amino acid (AA) tryptophan (TRP) diet plays critical role in physiological stress responses, neuropsychiatric health, oxidative systems, GI present study investigates relationship between varied...
ABSTRACT Toll-like receptor 2 (TLR2) deficiency enhances murine susceptibility to infection by Francisella tularensis as indicated accelerated mortality, higher bacterial burden, and greater histopathology. Analysis of pulmonary cytokine levels revealed that TLR2 results in significantly lower tumor necrosis factor alpha interleukin-6 but increased amounts gamma interferon monocyte chemoattractant protein 1. This pattern production may contribute the exaggerated pathogenesis seen −/− mice....
Consumption of human breast milk (HBM) attenuates the incidence necrotizing enterocolitis (NEC), which remains a leading and intractable cause mortality in preterm infants. Here, we report that this diminution correlates with alterations gut microbiota, particularly enrichment Propionibacterium species. Transfaunation microbiota from HBM-fed infants or newly identified cultured strain, P. UF1, to germfree mice conferred protection against pathogen infection correlated profound increases...
Translation of nanoparticles (NPs) into human clinical trials for patients with refractory cancers has lagged due to unknown biologic reactivities novel NP designs. To overcome these limitations, simple well-characterized mRNA lipid-NPs have been developed as cancer immunotherapeutic vaccines. While the preponderance RNA encoding tumor-associated antigens or neoepitopes designed target lymphoid organs, they remain encumbered by profound intratumoral and systemic immunosuppression that may...
The intestinal epithelium is equipped with sensing receptor mechanisms that interact luminal microorganisms and nutrients to regulate barrier function gut immune responses, thereby maintaining homeostasis. Herein, we clarify the role of extracellular calcium‐sensing (CaSR) using epithelium‐specific Casr −/− mice. Epithelial CaSR deficiency diminished function, altered microbiota composition, skewed responses towards proinflammatory. Consequently, mice were significantly more prone chemically...
The gram-negative bacterium Francisella tularensis survives in arthropods, fresh water amoeba, and mammals with both intracellular extracellular phases could reasonably be expected to express distinct phenotypes these environments. presence of a capsule on this has been controversial some groups finding such structure while other report that no identified. Previously we reported vitro culture conditions for which, contrast typical methods, yielded bacterial phenotype mimics the bacterium's...
Current thinking emphasizes the primacy of CD14 in facilitating recognition microbes by certain TLRs to initiate pro-inflammatory signaling events and importance p38-MAPK augmenting such responses. Herein, this paradigm is challenged demonstrating that live Borrelia burgdorferi not only triggers an inflammatory response absence CD14, but one is, part, a consequence altered PI3K/AKT/p38-MAPK impaired negative regulation TLR2. deficiency results increased localization PI3K lipid rafts,...
The current study was initiated when our specific-pathogen-free laboratory toms developed unexpectedly high levels of cross-reactive antibodies to human SARS-CoV-2 (SCoV2) receptor binding domain (RBD) upon mating with feline coronavirus (FCoV)-positive queens. Multi-sequence alignment analyses SCoV2 Wuhan RBD and four strains each from FCoV serotypes 1 2 (FCoV1 FCoV2) demonstrated an amino acid sequence identity 11.5% a similarity 31.8% FCoV1 (12.2% 36.5% for FCoV2 RBD). sera queens...
ABSTRACT Peripheral primitive neuroectodermal tumor (pPNET) is a very rare, highly malignant encountered in young dogs, with only four necropsy cases the veterinary literature. A 1.5‐year‐old male intact French Bulldog presented for evaluation of progressive left forelimb lameness and ipsilateral Horner's syndrome. Whole body computed tomography identified 12 cm mediastinal mass extension into vertebral canal multifocal metastatic lesions. Histological immunohistochemical results were...
Poor infiltration of CD8+ T cells and dysregulated MHC-I confer resistance to anticancer clinical therapies. This study aimed elucidate the mechanisms lysine-specific demethylase 1 (LSD1, encoded by KDM1A gene) in antitumor immunity Head Neck Squamous cell carcinoma (HNSCC). LSD1 inhibition syngeneic chronic tobacco carcinogen-induced HNSCC mice recruited activated dendritic (DCs), CD4+ cells, enriched interferon-gamma (IFNγ) CXCL9 DCs, CXCR3 as evaluated using flow cytometry single-cell...
Abstract Oral squamous cell carcinoma (OSCC) progresses from preneoplastic precursors via genetic and epigenetic alterations. Previous studies have focused on the treatment of terminally developed OSCC. However, role regulators as therapeutic targets during transition to OSCC has not been well studied. Our study identified lysine-specific demethylase 1 (LSD1) a crucial promoter OSCC, demonstrating that its knockout or pharmacological inhibition in mice reversed preneoplasia. LSD1 by SP2509...
Despite major advances in antiretroviral therapy against HIV-1, an effective HIV vaccine is urgently required to reduce the number of new cases infections world. Vaccines are ultimate tool medical arsenal control and prevent spread infectious diseases such as HIV/AIDS. Several failed phase-IIb -III clinical trials HIV-1 past generated a plethora information that could be used for better designing future. Most tested candidates produced strong humoral responses proteins; however, protect due...
Background Currently, sufficient data exist to support the use of lactobacilli as candidates for development new oral targeted vaccines. To this end, we have previously shown that Lactobacillus gasseri expressing protective antigen (PA) component anthrax toxin genetically fused a dendritic cell (DC)-binding peptide (DCpep) induced efficacious humoral and T cell-mediated immune responses against Bacillus anthracis Sterne challenge. Methodology/Principal Finding In present study, investigated...