A5070139186- Probiotics and Fermented Foods
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Gut microbiota and health
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Advanced Control Systems Optimization
- Transgenic Plants and Applications
- Antimicrobial Peptides and Activities
- RNA regulation and disease
- Protein purification and stability
- Enzyme Production and Characterization
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Innovation and Socioeconomic Development
- Biochemical and Structural Characterization
- Fault Detection and Control Systems
- Inflammatory Bowel Disease
North Carolina State University
2013-2024
North Central State College
2023-2024
ID Genomics (United States)
2015
Genomics (United Kingdom)
2014
Nutrition Sciences (Belgium)
2014
Norfolk State University
2012
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems in bacteria and archaea employ RNAs to specifically recognize the complementary DNA of foreign invaders, leading sequence-specific cleavage or degradation target DNA. Recent work has shown that accidental intentional targeting bacterial genome is cytotoxic can lead cell death. Here, we have demonstrated with CRISPR-Cas be employed for titratable removal individual strains species. Using type...
Clostridioides difficile is a bacterial pathogen responsible for significant morbidity and mortality across the globe. Current therapies based on broad-spectrum antibiotics have some clinical success, but approximately 30% of patients relapses, presumably due to continued perturbation gut microbiota. Here, we show that phages can be engineered with type I CRISPR-Cas systems modified reduce lysogeny enable specific efficient targeting killing C. in vitro vivo. Additional genetic engineering...
Significance The development of Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated genes (CAS)–based technology for targeted genome editing has revolutionized molecular biology approaches, but significant and outstanding gaps exist applications in bacteria, the native hosts these adaptive immune systems. This study shows that CRISPR-Cas systems can be directed to target delete genomic islands are flanked by insertion-sequence elements devoid essential genes....
Bacterial surface (S-) layers are crystalline arrays of self-assembling, proteinaceous subunits called S-layer proteins (Slps), with molecular masses ranging from 40 to 200 kDa. The S-layer-forming bacterium Lactobacillus acidophilus NCFM expresses three major Slps: SlpA (46 kDa), SlpB (47 kDa) and SlpX (51 kDa). has a demonstrated role in adhesion Caco-2 intestinal epithelial cells vitro, been shown modulate dendritic cell (DC) T-cell functionalities murine DCs. In this study, modification...
Bacteria encode clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated genes (cas), which collectively form an RNA-guided adaptive immune system against invasive genetic elements. In silico surveys have revealed that lactic acid bacteria harbour a prolific diverse set of CRISPR-Cas systems. Thus, the natural evolutionary role systems may be investigated in these ecologically, industrially, scientifically medically important microbes. this study, 17...
Surface proteins of probiotic microbes, including Lactobacillus acidophilus and gasseri, are believed to promote retention in the gut mediate host-bacterial communications. Sortase, an enzyme that covalently couples a subset extracellular containing LPXTG motif cell surface, is particular interest characterizing bacterial adherence communication with mucosal immune system. A sortase gene, srtA, was identified L. NCFM (LBA1244) gasseri ATCC 33323 (LGAS_0825). Additionally, eight six intact...
ABSTRACT CRISPR-Cas systems provide adaptive immunity against phages in prokaryotes via DNA-encoded, RNA-mediated, nuclease-dependent targeting and cleavage. Due to inefficient relatively limited DNA repair pathways bacteria, can be repurposed for lethal that selects sequence variants. In this study, the relative killing efficiencies of endogenous Type I II model organism Streptococcus thermophilus DGCC7710 were assessed. Additionally, genetic phenotypic outcomes chromosomal by...
The cellular and molecular mechanisms of inflammatory bowel disease are not fully understood; however, data indicate that uncontrolled chronic inflammation induced by bacterial gene products, including lipoteichoic acid (LTA), may trigger colonic resulting in pathogenesis. LTA is a constituent glycolipid Gram-positive bacteria shares many properties with lipopolysaccharide plays critical role the pathogenesis severe responses via Toll-like receptor 2. Accordingly, we elucidate immune...
Lactobacillus gasseri ATCC 33323 is a member of the acidophilus-complex group, microbes human origin with significant potential for impacting health based on niche-specific traits. In order to facilitate functional analysis this important species, upp-based counterselective chromosomal integration system was established and employed targeting lipoteichoic acid (LTA) synthesis gene, ltaS, in L. 33323. The ltaS gene encodes phosphoglycerol transferase responsible building glycerol chain LTA....
Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by decade of genomic and functional phenotypic investigations. L. deficient in lipoteichoic acid (LTA), major immunostimulant Gram-positive bacteria, has been shown to shift immune system responses animal disease models. However, the pleiotropic effects removing LTA from cell surface lactobacilli are unknown. In this study, we surveyed global transcriptional extracellular protein profiles two strains...
Process Analytical Technologies (PAT) used to monitor and control manufacturing processes are crucial for efficient automated bioprocessing, which is in congruence with lights-off-manufacturing Industry 4.0 initiatives. As biomanufacturing seeks realize more high-throughput operation, an increasing need multimodal analysis of process metrics becomes essential. Herein, we detail a series methods analyzing product yield from bioreactor how conduct cross-method comparisons. We employ model system