A5052177824- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Glycosylation and Glycoproteins Research
- Plant-Microbe Interactions and Immunity
- Biopolymer Synthesis and Applications
- Insect behavior and control techniques
- Entomopathogenic Microorganisms in Pest Control
- Bacterial biofilms and quorum sensing
- Insect Resistance and Genetics
- Infant Nutrition and Health
Research Triangle Park Foundation
2023-2024
AgBiome (United States)
2021-2024
Triangle
2021
North Carolina State University
2013-2020
Bacterial surface (S-) layers are crystalline arrays of self-assembling, proteinaceous subunits called S-layer proteins (Slps), with molecular masses ranging from 40 to 200 kDa. The S-layer-forming bacterium Lactobacillus acidophilus NCFM expresses three major Slps: SlpA (46 kDa), SlpB (47 kDa) and SlpX (51 kDa). has a demonstrated role in adhesion Caco-2 intestinal epithelial cells vitro, been shown modulate dendritic cell (DC) T-cell functionalities murine DCs. In this study, modification...
ABSTRACT Bacterial surface layers (S-layers) are crystalline arrays of self-assembling proteinaceous subunits called S-layer proteins (Slps) that comprise the outermost layer cell envelope. Many additional associated with or embedded within have been identified in Lactobacillus acidophilus NCFM, an S-layer-forming bacterium is widely used fermented dairy products and probiotic supplements. One putative S-layer-associated protein (SLAP), LBA0191, was predicted to mediate adhesion fibronectin...
ABSTRACT The Lactobacillus acidophilus homology group comprises Gram-positive species that include L. , helveticus crispatus amylovorus gallinarum delbrueckii subsp. bulgaricus gasseri and johnsonii . While these bacteria are closely related, they have varied ecological lifestyles as dairy food fermenters, allochthonous probiotics, or autochthonous commensals of the host gastrointestinal tract. Bacterial cell surface components play a critical role in molecular dialogue between interaction...
Health-promoting aspects attributed to probiotic microorganisms, including adhesion intestinal epithelia and modulation of the host mucosal immune system, are mediated by proteins found on bacterial cell surface. Notably, certain commensal bacteria contain a surface (S-) layer as outermost stratum wall. S-layers non-covalently bound semi-porous, crystalline arrays self-assembling, proteinaceous subunits called S-layer (SLPs). Recent evidence has shown that multiple co-localized within...
Abstract Africa accounts for a significant portion of the world’s sweetpotato production where it is widely grown as staple crop. In sub-Saharan (SSA), serves an important year-round source calories and nutrition, form income smallholder pre-commercial farmers, increasingly used silage animal feed. However, yield per hectare considerably lower in SSA than from other regions primarily due to weevils (SPW, Cylas spp., Coleoptera: Brentidae). Weevil feeding causes physical damage root can...
Autolysins, also known as peptidoglycan hydrolases, are enzymes that hydrolyze specific bonds within bacterial cell wall during division and daughter separation. Within the genome of Lactobacillus acidophilus NCFM, there 11 genes encoding proteins with hydrolase catalytic domains, 9 which predicted to be functional. Notably, 5 putative autolysins in L. NCFM S-layer-associated (SLAPs) noncovalently colocalized along surface (S)-layer at surface. One these SLAPs, AcmB, a...
Bacterial surface-layers (S-layers) are crystalline arrays of repeating proteinaceous subunits that coat the exterior many cell envelopes. S-layers have demonstrated diverse functions in growth and survival, maintenance integrity, mediation host interactions. Additionally, can act as scaffolds for outward display auxiliary proteins glycoproteins. These non-covalently bound S-layer associated (SLAPs) characterized roles division, adherence to intestinal cells, modulation immune response....
Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by decade of genomic and functional phenotypic investigations. L. deficient in lipoteichoic acid (LTA), major immunostimulant Gram-positive bacteria, has been shown to shift immune system responses animal disease models. However, the pleiotropic effects removing LTA from cell surface lactobacilli are unknown. In this study, we surveyed global transcriptional extracellular protein profiles two strains...