A5089228641- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Analytical chemistry methods development
- Mass Spectrometry Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Electrochemical Analysis and Applications
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Laser-induced spectroscopy and plasma
- Water Quality Monitoring and Analysis
- Mesenchymal stem cell research
- Plasma Diagnostics and Applications
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- CRISPR and Genetic Engineering
- Additive Manufacturing and 3D Printing Technologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Spectroscopy and Laser Applications
University of Florida
2016-2024
Allen Institute for Brain Science
2018-2020
Oxford University Press (United Kingdom)
2020
Pediatric Brain Tumor Foundation
2019
Valdosta State University
2012-2016
Mintek
1982-1991
With improving biofabrication technology, 3D bioprinted constructs increasingly resemble real tissues. However, the fundamental principles describing how cell-generated forces within these drive deformations, mechanical instabilities, and structural failures have not been established, even for basic biofabricated building blocks. Here we investigate behaviours of printed microbeams made from living cells extracellular matrix, bioprinting simple elements into a culture medium packed...
Abstract Background Despite advancements in the successful use of immunotherapy treating a variety solid tumors, applications brain tumors have lagged considerably. This is due, at least part, to lack well-characterized antigens expressed within that can mediate tumor rejection; low mutational burden these limits abundance targetable neoantigens; and immunologically “cold” microenvironment hampers generation sustained productive immunologic responses. The field mRNA-based therapeutics has...
Translation of nanoparticles (NPs) into human clinical trials for patients with refractory cancers has lagged due to unknown biologic reactivities novel NP designs. To overcome these limitations, simple well-characterized mRNA lipid-NPs have been developed as cancer immunotherapeutic vaccines. While the preponderance RNA encoding tumor-associated antigens or neoepitopes designed target lymphoid organs, they remain encumbered by profound intratumoral and systemic immunosuppression that may...
Immune checkpoint blockade using anti-PD-1 monoclonal antibodies has shown considerable promise in the treatment of solid tumors, but brain tumors remain notoriously refractory to treatment. In CNS malignancies that are completely resistant PD-1 blockade, we found bone marrow-derived, lineage-negative hematopoietic stem and progenitor cells (HSCs) express C-C chemokine receptor type 2 (CCR2+) reverses resistance sensitizes mice curative immunotherapy. HSC transfer with increases T-cell...
Immunotherapy has been demonstrably effective against multiple cancers, yet tumor escape is common. It remains unclear how brain tumors immunotherapy and to overcome this immune escape.
Extensive clonal T cell expansion is associated with response to immunotherapy.
Kryptolebias marmoratus is a synchronous hermaphroditic vertebrate that utilizes an ovotestis for reproduction. This fish develops externally, easy to maintain, and has about 100-day life cycle, making it desirable developmental genetic model organism. Here, we present pilot zygotic mutant screen utilizing the common chemical mutagen, N-ethyl-N-nitrosourea (ENU) establish genetics in this species. Selection of clonal stocks optimal conditions mutagenizing are presented types frequencies...
Background Glioma-induced immune dysregulation of the hematopoietic system has been described in a limited number studies. In this study, our group further demonstrates that gliomas interrupt cellular differentiation programming and outcomes stem progenitor cells (HSPCs) bone marrow. HSPCs from glioma-bearing mice are reprogrammed driven towards expansion myeloid lineage precursors myeloid-derived suppressor (MDSCs) secondary lymphoid organs. However, we found is reversed by immunotherapy....
The mangrove killifish, Kryptolebias marmoratus, is unique among vertebrates due to its self-fertilizing mode of reproduction involving an ovotestis. As a result, it constitutes simplistic and desirable vertebrate model for developmental genetics as easily maintained, reaches sexual maturity in about 100 days, provides manageable number relatively clear embryos. After the establishment characterization initial mutagenesis pilot screen using N-ethyl-N-nitrosourea, three-generation genetic was...
Abstract INTRODUCTION Glioblastoma (GBM) contains cell populations with distinct metabolic requirements, fast-cycling cells harnessing aerobic glycolysis, and treatment-resistant slow-cycling (SCCs) preferentially engaging lipid metabolism. How the different tumor interact immune how this heterogeneity shapes landscape in GBM has yet to be understood. OBJECTIVES The objectives are unravel various molecular signals link that underlie interaction of SCCs microenvironment, particular...
Abstract INTRODUCTION: Immunotherapy for GBM has resulted in limited benefits to overall survival due the targeting of antigens and hostile TME. Our group developed a patented vaccine platform that combines an mRNA-nanoparticle with CXCL9 loaded polyethylene glycol (PEG) hydrogel. The HCM is given SQ recruits diverse immune cell population deliver large payload mRNA. objective these studies was evaluate efficacy mechanisms action vaccine. METHODS: C57BL/6 mice underwent intracranial...
<p>Supplementary Data</p>
<div>AbstractPurpose:<p>Immunotherapy has been demonstrably effective against multiple cancers, yet tumor escape is common. It remains unclear how brain tumors immunotherapy and to overcome this immune escape.</p>Experimental Design:<p>We studied KR158B-luc glioma-bearing mice during treatment with adoptive cellular therapy (ACT) polyclonal tumor-specific T cells. We tested the immunogenicity of primary escaped using T-cell restimulation assays. used flow cytometry...
<div>AbstractPurpose:<p>Immunotherapy has been demonstrably effective against multiple cancers, yet tumor escape is common. It remains unclear how brain tumors immunotherapy and to overcome this immune escape.</p>Experimental Design:<p>We studied KR158B-luc glioma-bearing mice during treatment with adoptive cellular therapy (ACT) polyclonal tumor-specific T cells. We tested the immunogenicity of primary escaped using T-cell restimulation assays. used flow cytometry...
<p>Supplementary Data</p>
Abstract INTRODUCTION We previously published that hematopoietic stem cell combination therapy (HSC+aPD1) extends survival in murine CNS malignancies, including glioma. To better understand lymphoid-specific mechanisms, we analyzed various lymphoid tissues a glioma model. hypothesize early PD-1 blockade HSCs influences later differentiation trajectories. Transferred differentiate into T-cells within the thymus and then migrate to intracranial tumor microenvironment as anticipated. Indeed,...
Abstract Background: The development of successful immunotherapy targeting antigens in glioblastoma multiforme (GBM) remains a challenge owing to antigen heterogeneity and the low mutation burden GBM. Cancer immunogenomics represents complementary approach application genomics developing novel immunotherapies for Our goal is develop personalized RNA-based therapeutic vaccine that simultaneously targets selected pool tumor rejection including all neoantigens tumor-associated (TAAs) as...