A5069494939- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Chemokine receptors and signaling
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Brain Metastases and Treatment
- Cancer Research and Treatments
- Influenza Virus Research Studies
- Metabolomics and Mass Spectrometry Studies
- Immune Response and Inflammation
- Scientific Computing and Data Management
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Breastfeeding Practices and Influences
- Research Data Management Practices
- Photoacoustic and Ultrasonic Imaging
- Photodynamic Therapy Research Studies
- Protein Tyrosine Phosphatases
- Health, Environment, Cognitive Aging
- Infant Nutrition and Health
University of Florida
2016-2024
Florida College
2016-2023
Therapeutics Clinical Research
2022
Neurological Surgery
2021
Pediatric Brain Tumor Foundation
2019
Allen Institute for Brain Science
2016-2018
The changes induced in host immunity and the tumor microenvironment by chemotherapy have been shown to impact immunotherapy response both a positive negative fashion. Temozolomide is most common used treat glioblastoma (GBM) has variable effects on immune immunotherapy. Therefore, we aimed determine modulatory of temozolomide that would checkpoint inhibition treatment experimental GBM.Immune function antitumor efficacy were tested after with metronomic dose (MD) (25 mg/kg × 10 days) or...
Spinal cord gliomas are rare entities that often have limited surgical options. Immunotherapy has shown promise in intracranial with some research suggesting benefit for spinal gliomas. A focused review of immunotherapies been investigated was performed. The primary methods immunotherapy include immune checkpoint inhibitors, adoptive T-cell therapies, and vaccine strategies. There innumerable challenges must be overcome to effectively apply immunotherapeutic strategies the including low...
<h3>Background</h3> Dendritic cell (DC) vaccine efficacy is directly related to the efficiency of DC migration lymph node after delivery patient. We discovered that a naturally occurring metabolite, sarcosine, increases in human and murine cells resulting significantly improved anti-tumor efficacy. hypothesized sarcosine induced was due chemokine signaling. <h3>Methods</h3> DCs were harvested from bone marrow wild type C57BL/6 mice electroporated with tumor messenger RNA (mRNA). Human...
In oncology, "immunotherapy" is a broad term encompassing multiple means of utilizing the patient's immune system to combat malignancy. Prominent among these are checkpoint inhibitors, cellular therapies including chimeric antigen receptor T-cell therapy, vaccines, and oncolytic viruses. Immunotherapy for glioblastoma (GBM) has had mixed results in early trials. this context, past, present, future oncology treatment GBM was discussed by clinical, research, thought leaders as well patient...
Abstract The transition of effector T cells or memory precursors into distinct long-lived cell subsets is not well understood. Although many molecules made by APCs can contribute to clonal expansion and differentiation, it clear if contraction development passive active. Using respiratory virus infection, we found that CD8 cannot express the TNF family molecule lymphotoxin-like, exhibits inducible expression, competes with HSV glycoprotein D for herpes entry mediator, a receptor expressed...
How tissue-specific anatomical distribution and phenotypic specialization are linked to protective efficacy of memory T cells against reinfection is unclear. Here, we show that lung environmental cues program recently recruited central-like with migratory potentials for their functions during lethal respiratory virus infection. After entering the lung, some retain original CD27hiCXCR3hi phenotype, enabling them localize near infected bronchiolar epithelium airway lumen function as first line...
PTPN22 (protein tyrosine phosphatase non receptor 22) encodes a that functions as key regulator of immune homeostasis. In particular, inhibits T‐cell signaling and selectively promotes type I interferon responses in myeloid cells. To date, there is little information on the CD8 T‐cell‐intrinsic role response to viral pathogen. We unexpectedly found PTPN22‐deficient virus‐specific T cells failed accumulate wild‐type hosts after lymphocytic choriomeningitis virus infection. Lack expression...
OBJECTIVE The objective of this study was to develop a murine system for the delivery laser interstitial thermotherapy (LITT) with probe-based thermometry as model human glioblastoma treatment investigate thermal diffusion in heterogeneous brain tissue. METHODS First, tissue heating properties were characterized using diode-pumped solid-state near-infrared homogeneous model. adapted use repurposed stereotactic surgery frame utilizing micro probe and Hamilton syringe. authors designed...
Abstract INTRODUCTION Intra-tumoral immunosuppression is a major cause of treatment failure for patients with glioblastoma (GBM). Our group has developed proprietary vaccine that combines mRNA encapsulated in lipid nanoparticles PEG hydrogel and chemokine (CXCL9). This hydrogel-chemokine-mRNA (HCM) delivered subcutaneously (SQ) results significant anti-tumor efficacy syngeneic murine glioma models. The objective this study was to evaluate the effects on tumor microenvironment (TME). METHODS...
Liquid chromatography-mass spectrometry (LC-MS) metabolomics studies produce high-dimensional data that must be processed by a complex network of informatics tools to generate analysis-ready sets. As the first computational step in metabolomics, processing is increasingly becoming challenge for researchers develop customized workflows are applicable LC-MS analysis. Ontology-based automated workflow composition (AWC) systems provide feasible approach developing consume molecular data. We used...
Glioblastoma is characterized by treatment resistance resulting in aggressive growth. Due to limited efficacy of current treatments, novel strategies are under investigation. In this study, we hypothesised that dose modified TMZ improves GBM response immune check point blockade priming host immunity and changing tumor microenvironment. Murine glioma cell lines were treated with checked for PD-1, PDL-1. mice injected different doses evaluate PDL-1 lymphocytes at time points post TMZ. recieved...
Abstract INTRODUCTION Our group and others have shown mRNA-vaccines significant anti-tumor efficacy in the treatment of brain tumors are currently being tested first-in-human trials. To further enhance mRNA delivery, a hydrogel platform was developed with addition CXCL9 to promote immune cell trafficking. METHODS We generated vaccine by utilizing platform. Nano-mRNA were loaded into hydrogel. In vitro recruitment DCs NK evaluated fluorescence microscopy. vivo cells analyzed flowcytometry...
Based on our previous publication in Nature, we have established that increasing dendritic cell (DC) migration glioblastoma (GBM) patients treated with DC vaccination results significantly improved survival. In this project evaluated the efficacy of using a non-toxic, naturally occurring metabolite called sarcosine to increase cellular setting immunotherapy. In-vitro was analyzed cytotoxic transfer membrane. vivo by vaccinating murine model. C57BL/6 mice received tetanus diphtheria (Td)...
Immunotherapy with adoptive transfer T cells is an effective treatment for cancer. We have recently demonstrated the ability to overcome chemotherapy resistance in a mouse model of malignant glioma cell. This platform dependent on migration tumor site. Therefore, tracking transferred critical evaluation immunotherapy efficacy. The objective this project was establish novel method track immune vivo using 13C-labeling nucleic acids. Human were isolated from peripheral blood mononuclear...
Abstract INTRODUCTION The adaptive immune response requires robust T cell proliferation and activation. These changes are dependent on metabolic program shifts that can be measured using metabolomic analysis. objective of this project was to identify a metabolomics profile serve as biomarker immunotherapy for the treatment brain tumors. METHODS GL261-gp100 tumor-bearing mice were received anti-PD1 or bone marrow-derived dendritic (DC) vaccine generated ex vivo. Urine samples collected...
Abstract INTRODUCTION: Immunotherapy for GBM has resulted in limited benefits to overall survival due the targeting of antigens and hostile TME. Our group developed a patented vaccine platform that combines an mRNA-nanoparticle with CXCL9 loaded polyethylene glycol (PEG) hydrogel. The HCM is given SQ recruits diverse immune cell population deliver large payload mRNA. objective these studies was evaluate efficacy mechanisms action vaccine. METHODS: C57BL/6 mice underwent intracranial...