A5029771729- Neurogenesis and neuroplasticity mechanisms
- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Immune cells in cancer
- Diet and metabolism studies
- Cancer Research and Treatments
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Amino Acid Enzymes and Metabolism
- RNA Interference and Gene Delivery
- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Hedgehog Signaling Pathway Studies
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
University of Florida
2016-2025
Mayo Clinic in Florida
2024-2025
Nemours Children's Clinic
2025
Florida College
2014-2024
Allen Institute for Brain Science
2011-2024
Reynolds American (United States)
2016
Pediatric Brain Tumor Foundation
2016
Stem Cell Institute
2015
Cardiff University
2015
Cancer Center Amsterdam
2012
Glioblastoma remains one of the most lethal types cancer, and is common brain tumour in adults. In particular, recurrence after surgical resection radiation invariably occurs regardless aggressive chemotherapy. Here, we provide evidence that transcription factor ZEB1 (zinc finger E-box binding homeobox 1) exerts simultaneous influence over invasion, chemoresistance tumourigenesis glioblastoma. preferentially expressed invasive glioblastoma cells, where ZEB1-miR-200 feedback loop...
Abstract Chimeric antigen receptor (CAR) T-cell therapy targeting solid tumors has stagnated as a result of tumor heterogeneity, immunosuppressive microenvironments, and inadequate intratumoral T cell trafficking persistence. Early (≤3 days) presentation CAR cells post-treatment is superior predictor survival than peripheral Therefore, we have co-opted IL-8 release from to enhance through design for maximal antitumor activity in tumors. Here, demonstrate that receptor, CXCR1 or CXCR2,...
Abstract Advancement in our understanding of the biology adult stem cells and their therapeutic potential relies heavily on meaningful functional assays that can identify measure cell activity vivo vitro. In mammalian nervous system, neural (NSCs) are often studied using a culture system referred to as neurosphere assay. We previously challenged central tenet this assay, all neurospheres derived from NSC, provided evidence it overestimates NSC frequency, rendering inappropriate for...
Individual tumour cells display diverse functional behaviours in terms of proliferation rate, cell–cell interactions, metastatic potential and sensitivity to therapy. Moreover, sequencing studies have demonstrated surprising levels genetic diversity between individual patient tumours the same type. Tumour heterogeneity presents a significant therapeutic challenge as cell types within can respond differently therapies, inter-patient may prevent development general treatments for cancer. One...
// Parvinder Hothi 1 , Timothy J. Martins 2 LiPing Chen Loic Deleyrolle 3 Jae-Geun Yoon Brent Reynolds and Greg Foltz The Ben Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute, Seattle, WA, USA Quellos High-throughput Screening Core, UW Medicine, McKnight University of Florida, Gainesville, FL, Correspondence: Foltz, email: Keywords : disulfiram, glioblastoma, high-throughput chemical screens, stem cells Received October 12, 2012, Accepted 21, Published...
The coordination of complex tumor processes requires cells to rapidly modify their phenotype and is achieved by direct cell-cell communication through gap junction channels composed connexins. Previous reports have suggested that junctions are suppressive based on connexin 43 (Cx43), but this does not take into account differences in connexin-mediated ion selectivity intercellular rate drive diversity. We find glioblastoma cancer stem (CSCs) possess functional can be targeted using...
Dysregulated energetics coupled with uncontrolled proliferation has become a hallmark of cancer, leading to increased interest in metabolic therapies. Glioblastoma (GB) is highly malignant, very metabolically active, and typically resistant current Dietary treatment options based on glucose deprivation have been explored using restrictive ketogenic diet (KD), positive anticancer reports. However, negative side effects lack palatability make the KD difficult implement an adult population....
Anti-VEGF therapy prolongs recurrence-free survival in patients with glioblastoma but does not improve overall survival. To address this discrepancy, we investigated immunologic resistance mechanisms to anti-VEGF glioma models. A screening of immune-associated alterations tumors after treatment revealed a dose-dependent upregulation regulatory T-cell (Treg) signature genes. Enhanced numbers Tregs were observed spleens tumor-bearing mice and later treatment. Elimination CD25 blockade before...
Glioblastoma (GBM) is the most common and malignant primary brain tumor, resulting in poor survival despite aggressive therapies. GBM characterized part by a highly heterogeneous immunosuppressive tumor microenvironment (TME) made up predominantly of infiltrating peripheral immune cells. One significant cell type that contributes to glioma evasion population immunosuppressive, hematopoietic cells, termed myeloid-derived suppressor cells (MDSCs). Previous studies suggest potent subset myeloid...
Protein arginine methyltransferase 5 (PRMT5), which symmetrically dimethylates cytosolic and nuclear proteins, has been demonstrated as an important cancer therapeutic target. In recent years, many advanced achievements in PRMT5 inhibitor development have made. Most inhibitors the clinical trial focus on targeting C-terminal catalytic domain, whereas developing small molecules to interrupt PRMT5/pICLn (methylosome subunit) protein–protein interface is also of great importance for inhibiting...
Abstract Adult human and rodent brains contain neural stem progenitor cells, the presence of cells in adult spinal cord has also been described. Here, using electron microscopy, expression precursor cell markers, culture, we investigated whether are present cord. In well‐preserved nonpathological post‐mortem cord, nestin, Sox2, GFAP, CD15, Nkx6.1, PSA‐NCAM were found to be expressed heterogeneously by located around central canal. Ultrastructural analysis revealed existence immature close...
Abstract Tumor-initiating cells (TIC) perpetuate tumor growth, enable therapeutic resistance, and drive initiation of successive tumors. Virtually nothing is known about the role mechanotransductive signaling in controlling TIC tumorigenesis, despite recognized importance altered mechanics tissue dysplasia common observation that extracellular matrix (ECM) stiffness strongly regulates cell behavior. To address this open question, we cultured primary human glioblastoma (GBM) TICs on...
Representing a renewable source for cell replacement, neural stem cells have received substantial attention in recent years. The neurosphere assay represents method to detect the presence of cells, however owing deficiency specific and definitive markers identify them, their quantification rate they expand is still indefinite. Here we propose mathematical interpretation allowing actual measurement symmetric division frequency. algorithm modeling demonstrates direct correlation between...
The Y-box binding protein 1 (YB-1) is upregulated in many human malignancies including glioblastoma (GBM). It also essential for normal brain development, suggesting that YB-1 part of a neural stem cell (NSC) network. Here, we show was highly expressed the subventricular zone (SVZ) mouse fetal tissues but not terminally differentiated primary astrocytes. Conversely, knockout mice had reduced Sox-2, nestin, and musashi-1 expression SVZ. Although murine neurospheres were rich YB-1, its lost...
Tumor migration/metastasis and immunosuppression are major obstacles in effective cancer therapy. Incidentally, these 2 hurdles usually coexist inside tumors, therefore making therapy significantly more complicated, as both oncogenic mechanisms must be addressed for successful therapeutic intervention. Our recent report highlights that the tumor expression of a TNF family member, CD70, is correlated with poor survival primary gliomas. In this study, we investigated how CD70 by GBM affects...
Translation of nanoparticles (NPs) into human clinical trials for patients with refractory cancers has lagged due to unknown biologic reactivities novel NP designs. To overcome these limitations, simple well-characterized mRNA lipid-NPs have been developed as cancer immunotherapeutic vaccines. While the preponderance RNA encoding tumor-associated antigens or neoepitopes designed target lymphoid organs, they remain encumbered by profound intratumoral and systemic immunosuppression that may...
A better understanding of the molecules implicated in growth and survival glioblastoma (GBM) cells their response to temozolomide (TMZ), standard-of-care chemotherapeutic agent, is necessary for development new therapies that would improve outcome current GBM treatments. In this study, we characterize role pericentriolar material 1 (PCM1), a component centriolar satellites surrounding centrosomes, cell proliferation sensitivity genotoxic agents such as TMZ. We show PCM1 expressed around...
Certain limitations of the neurosphere assay (NSA) have resulted in a search for alternative culture techniques brain tumor-initiating cells (TICs). Recently, reports described growing glioblastoma (GBM) TICs as monolayer using laminin. We performed side-by-side analysis NSA and laminin (adherent) conditions to compare growth expansion GBM TICs. were grown adherent conditions. Comparisons made culture, apoptosis assays, protein expression, limiting dilution clonal frequency assay, genetic...
Stem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue all these tumors, especially glioblastoma mutliforme (GBM), is to identify isolate tumor initiating cell population(s) investigate their role formation, progression, recurrence. Understanding populations will provide clues finding effective therapeutic approaches for tumors. The neurosphere assay (NSA) due its simplicity reproducibility has used the method of choice isolation...