A5023868966- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cell Image Analysis Techniques
- Receptor Mechanisms and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Ion Channels and Receptors
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Calcium signaling and nucleotide metabolism
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- Neurological diseases and metabolism
- Health, Environment, Cognitive Aging
- Dendrimers and Hyperbranched Polymers
- Bacterial biofilms and quorum sensing
- Renal Diseases and Glomerulopathies
- Bacterial Identification and Susceptibility Testing
- Force Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Cardiac electrophysiology and arrhythmias
- Parkinson's Disease Mechanisms and Treatments
- Pluripotent Stem Cells Research
University of Münster
2010-2023
University Hospital Münster
2013
Max Planck Society
2005-2010
Max Planck Institute for Biophysical Chemistry
2005-2010
Saarland University
2005
Bogomoletz Institute of Physiology
1997-2004
National Academy of Sciences of Ukraine
1997-2000
Phenotypic drug discovery requires billions of cells for high-throughput screening (HTS) campaigns. Because up to several million different small molecules will be tested in a single HTS campaign, even variability within the cell populations could easily invalidate an entire campaign. Neurodegenerative assays are particularly challenging because neurons post-mitotic and cannot expanded implementation HTS. Therefore, neuroprotective compounds type that is robustly expandable able...
Three-dimensional (3D) culture systems have fueled hopes to bring about the next generation of more physiologically relevant high-throughput screens (HTS). However, current protocols yield either complex but highly heterogeneous aggregates (‘organoids’) or 3D structures with less physiological relevance (‘spheroids’). Here, we present a scalable, HTS-compatible workflow for automated generation, maintenance, and optical analysis human midbrain organoids in standard 96-well-plates. The...
Abstract Background Different non-invasive real-time imaging techniques have been developed over the last decades to study bacterial pathogenic mechanisms in mouse models by following infections a time course. In vivo investigations of previously relied mostly on bioluminescence (BLI), which is able localize metabolically active bacteria, but provides no data status involved organs infected host organism. this we established an platform magnetic resonance (MRI) for tracking bacteria...
Compensatory endocytosis is crucial for the presynapse to maintain a functional pool of fusion-competent vesicles. Slow Clathrin-mediated has been widely regarded as primary mechanism retrieval but this challenged by competing Clathrin-independent endocytic models, most notably sub-second ultra-fast endocytosis, reported be predominant at physiological temperature. Here, we sought resolve salience respective modes using purely presynaptic preparation, Xenapse, amenable total internal...
Neurons use Clathrin-mediated endocytosis to retrieve synaptic vesicle (SV) proteins during compensatory after presynaptic SV fusion. We have shown cargo be re-sorted and pre-assembled outside the active zone into Clathrin-coated structures (CCS) of variable size curvature, constituting a readily retrievable pool. During CCS pool must remodeled swiftly spherical vesicles within 10 seconds at physiological temperature. How this is achieved remains elusive. Here we performed live-cell imaging...
The primary unit of exocytosis, the synaptic vesicle, is replete with proteins which enable vesicle fusion. For maintaining transmission have to be rapidly cleared from active zone. immediate fate cargo post-fusion and precise mechanism its resorting into a readily retrievable pool (RRetP) for endocytosis remains unclear. To address this, we developed purely presynaptic preparation, xenapse, boutons formed en face directly onto glass coverslip enabling optical single recording by total...
During the development of mammalian neocortex, generation neurons by neural progenitors and their migration to final position are closely coordinated. The highly polarized radial glial cells (RGCs) serve both as progenitor generate support for these neurons. After generation, transiently assume a multipolar morphology before they polarize begin along RGCs. Here, we show that Rap1 GTPases perform essential functions cortical organization master regulators cell polarity. Conditional deletion...
The effects on the whole‐cell carbachol‐induced muscarinic cationic current (mIcat) of antibodies against α ‐subunits various G proteins, as well effect a βγ subunit, were studied in single guinea‐pig ileal smooth muscle cells voltage‐clamped at −50 mV. Ionized intracellular calcium concentration, [Ca 2+ ] i , was clamped 100 n M using 1,2‐bis(2‐aminophenoxyl‐ethane‐ N ′, ′‐tetraacetic acid)/Ca mixture. Application ascending concentrations carbachol (1–300 μ ) activated mIcat (mean amplitude...
The establishment of a polarized morphology is essential for the development and function neurons. During mammalian neocortex, neurons arise in ventricular zone (VZ) from radial glia cells (RGCs) leave VZ to generate cortical plate (CP). their migration, newborn first assume multipolar subventricular (SVZ) lower intermediate (IZ). Subsequently, they undergo multi-to-bipolar (MTB) transition become bipolar upper IZ by developing leading process trailing axon. small GTPases Rap1A Rap1B act as...
In visceral smooth muscles, both M 2 and 3 muscarinic receptor subtypes are found, produce two major metabolic effects: adenylyl cyclase inhibition PLC β activation. Thus, we studied their relevance for cationic current (mI CAT ) generation, which underlies cholinergic excitation. Experiments were performed on single guinea‐pig ileal cells using patch‐clamp recording techniques under conditions of weakly buffered [Ca 2+ ] i (either 50 μ EGTA or 50–100 fluo‐3 confocal fluorescence imaging)...
Significance Statement Endocytosis, recycling, and degradation of proteins are essential functions mammalian cells, especially for terminally differentiated cells with limited regeneration rates complex morphology, such as podocytes. To improve our understanding on how disturbances these trafficking pathways linked to podocyte depletion slit diaphragm (SD) injury, the authors explored role small GTPase Rab7, which is endosomal, lysosomal, autophagic pathways, using model systems mice...
Abstract The topography of biological membranes is critical for formation protein and lipid microdomains. One prominent example in the yeast plasma membrane (PM) are BAR domain‐induced PM furrows. Here we report a novel function Sur7 family tetraspanner proteins regulation local topography. Combining TIRF imaging, STED nanoscopy, freeze–fracture EM simulations find that tetraspanners form multimeric strands at edges furrows, where they modulate forces exerted by domain furrow base. Loss or...
The effects of SK&F 96365 on cationic current evoked either by activating muscarinic receptors with carbachol or intracellularly applied GTPgammaS (in the absence carbachol) were studied using patch-clamp recording techniques in single guinea-pig ileal smooth muscle cells. reversibly inhibited receptor a concentration-, time- and voltage-dependent manner producing concomitant alteration steady-state I-V relationship shape which could be explained assuming that increasing membrane positivity...