A5000459369- Advanced MRI Techniques and Applications
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Atomic and Subatomic Physics Research
- Orthopedic Infections and Treatments
- Lanthanide and Transition Metal Complexes
- MRI in cancer diagnosis
- Cardiac Imaging and Diagnostics
- Medical Imaging Techniques and Applications
- Advanced NMR Techniques and Applications
- Bacterial biofilms and quorum sensing
- Cardiovascular Function and Risk Factors
- Radiomics and Machine Learning in Medical Imaging
- Streptococcal Infections and Treatments
- Electron Spin Resonance Studies
- Metabolomics and Mass Spectrometry Studies
- Renal Transplantation Outcomes and Treatments
- Dietary Effects on Health
- Thermal Regulation in Medicine
- Iron Metabolism and Disorders
- Gut microbiota and health
- Research on Leishmaniasis Studies
- Hemoglobinopathies and Related Disorders
- Diet and metabolism studies
University Hospital Bonn
2021-2024
University Hospital Münster
2012-2023
University of Münster
2012-2023
Friedrich Schiller University Jena
2016-2023
Jena University Hospital
2015-2023
University of Bonn
2022
University of Calgary
2012
University of Würzburg
2004-2010
Medical Mission Institute
2006-2007
Queen's University Belfast
2007
Staphylococcus aureus osteomyelitis often develops to chronicity despite antimicrobial treatments that have been found be susceptible in vitro tests. The complex infection strategies of S. aureus, including host cell invasion and intracellular persistence via the formation dynamic small colony variant (SCV) phenotypes, could responsible for therapy-refractory courses. To analyse efficacy antibiotics acute chronic stage bone infections, we established long-term vivo models. Antibiotics were...
Dietary restriction (DR) improves health, delays tissue aging, and elongates survival in flies worms. However, studies on laboratory mice nonhuman primates revealed ambiguous effects of DR lifespan despite improvements health parameters. In this study, we analyzed consequences adult-onset (24 h to 1 yr) hematopoietic stem cell (HSC) function. ameliorated HSC aging phenotypes, such as the increase number HSCs skewing toward myeloid-biased during aging. Furthermore, increased quiescence...
The emergence of antibiotic resistant pathogenic bacteria has reduced our ability to combat infectious diseases. At the same time numbers new antibiotics reaching market have decreased. This situation created an urgent need discover novel scaffolds. Recently, application pattern recognition techniques identify molecular fingerprints in 'omics' studies, emerged as important tool biomedical research and laboratory medicine pathogens, monitor therapeutic treatments or develop drugs with...
Metabolomics has become an important tool to study host-pathogen interactions and discover potential novel therapeutic targets. In attempt develop a better understanding of the process pathogenesis associated host response we have used quantitative 1H NMR approach metabolic different bacterial infections. Here describe that changes found in serum mice were infected with Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli Pseudomonas aeruginosa can distinguish between infections...
Abstract Background Different non-invasive real-time imaging techniques have been developed over the last decades to study bacterial pathogenic mechanisms in mouse models by following infections a time course. In vivo investigations of previously relied mostly on bioluminescence (BLI), which is able localize metabolically active bacteria, but provides no data status involved organs infected host organism. this we established an platform magnetic resonance (MRI) for tracking bacteria...
The ferritin heavy/heart chain (FTH) gene encodes the ferroxidase component of iron (Fe) sequestering complex, which plays a central role in regulation cellular Fe metabolism. Here we tested hypothesis that regulates organismal metabolism manner impacts energy balance and thermal homeostasis.We developed mouse strain, referred herein as FthR26 fl/fl, expressing tamoxifen-inducible Cre recombinase under control Rosa26 (R26) promoter carrying two LoxP (fl) sites: one at 5'end Fth another 3'...
Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types tissue For this, we collected 10–12 clinical S. strains each from nasal colonization, and patients endoprosthesis infection, hematogenous osteomyelitis, sepsis. All were characterized by genotypic analysis, the expression factors. host–pathogen interaction studied through several functional assays in...
Heart failure is associated with altered myocardial substrate metabolism and impaired cardiac energetics. Comorbidities like diabetes may influence the metabolic adaptations during heart development. We quantified to what extent changes in preference, lipid accumulation, energy status predict longitudinal development of hypertrophy non-diabetic diabetic heart.Transverse aortic constriction (TAC) was performed (db/+) (db/db) mice induce pressure overload. Magnetic resonance imaging, 31P...
Background The inflammatory tumor microenvironment (TME) is formed by various immune cells, being closely associated with tumorigenesis. Especially, the interaction between tumor-infiltrating T-cells and macrophages has a crucial impact on progression metastatic spread. purpose of this study was to investigate whether oscillating-gradient diffusion-weighted MRI (OGSE-DWI) enables cell size-based discrimination different populations TME. Methods Sine-shaped OGSE-DWI combined Imaging...
To overcome flow and electrocardiogram-trigger artifacts in cardiovascular magnetic resonance (CMR), we have implemented a cardiac respiratory self-gated cine ultra-short echo time (UTE) sequence. We assessed its performance healthy mice by comparing the results with those obtained fast low angle shot (FLASH) sequence echocardiography. 2D UTE (TE/TR = 314 μs/6.2 ms, resolution: 129 × μm, scan per slice: 5 min sec) FLASH 3 ms/6.2 4 49 images were acquired at 9.4 T. Volume of left right...
Time resolved 4D phase contrast (PC) cardiovascular magnetic resonance (CMR) in mice is challenging due to long scan times, small animal ECG-gating and the rapid blood flow cardiac motion of rodents. To overcome several these technical challenges we implemented a retrospectively self-gated PC radial ultra-short echo-time (UTE) acquisition scheme assessed its performance healthy by comparing results with those obtained an ECG-triggered fast low angle shot (FLASH) sequence. Cardiac CMR images...
Abstract At high magnetic fields diagnostic proton MRI of the lung is problematic, because fast T relaxation. The application superparamagnetic contrast agents and exploitation corresponding effect inefficient with conventional methods, which limits early detection diseases. However, a simple theoretical treatment shows that in lung, by use ultra‐short echo time sequences, effects can be neglected while 1 shortening used for signal detection. In our study, we have applied theoretically...
Staphylococcus aureus causes severe infections associated with inflammation, such as sepsis or osteomyelitis. Inflammatory processes are regulated by distinct lipid mediators (LMs) but how their biosynthetic pathways orchestrated in S. is elusive. We show that strikingly not only modulates pro-inflammatory, also inflammation-resolving LM murine osteomyelitis and osteoclasts well human monocyte-derived macrophages (MDMs) different phenotype. Targeted metabololipidomics using ultra-performance...
Background Preventing sepsis‐associated acute kidney injury (S‐AKI) can be challenging because it develops rapidly and is often asymptomatic. Probability assessment of disease progression for therapeutic follow‐up outcome are important to intervene prevent further damage. Purpose To establish a noninvasive multiparametric MRI (mpMRI) tool, including T 1 , 2 perfusion mapping, probability the S‐AKI. Study Type Preclinical randomized prospective study. Animal Model One hundred forty adult...
Response assessment of targeted cancer therapies is becoming increasingly challenging, as it not adequately assessable with conventional morphological and volumetric analyses tumor lesions. The microenvironment particularly constituted by vasculature which altered various therapies. aim this study was to noninvasively assess changes in perfusion vessel permeability after therapy murine models breast divergent degrees malignancy.Low malignant 67NR or highly 4T1 tumor-bearing mice were treated...
Infective endocarditis (IE) is a severe and often fatal disease, lacking fast reliable diagnostic procedure. The purpose of this study was to establish mouse model Staphylococcus aureus-induced IE develop MRI technology characterize diagnose IE. To the hematogenous IE, aortic valve damage induced by placing permanent catheter into right carotid artery. 24 h after surgery, mice were injected intravenously with either iron particle-labeled or unlabeled S. aureus (strain 6850). distinguish...
The objective of this study was to non-invasively differentiate the degree malignancy in two murine breast cancer models based on identification distinct tissue characteristics a metastatic and non-metastatic tumor model using multiparametric Magnetic Resonance Imaging (MRI) approach.The highly 4T1 compared 67NR model. conducted 9.4 T small animal MRI. protocol used characterize tumors regarding their structural composition, including heterogeneity, intratumoral edema hemorrhage, as well...
In this study, we established and validated a time-resolved three-dimensional phase-contrast magnetic resonance imaging method (4D PC MRI) on 9.4 T small-animal MRI system. Herein present the feasibility of 4D in terms qualitative quantitative flow pattern analysis mice with transverse aortic constriction (TAC). FLASH images phantom mouse heart were acquired at using four-point phase-encoding scheme. The was compared slice-selective ultrasound Bland–Altman analysis. Advanced 3D streamlines...