A5054087402- Advanced MRI Techniques and Applications
- Medical Imaging Techniques and Applications
- MRI in cancer diagnosis
- Advanced Neuroimaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Photoacoustic and Ultrasonic Imaging
- Immune cells in cancer
- Advanced Electron Microscopy Techniques and Applications
- Ultrasound Imaging and Elastography
- Atomic and Subatomic Physics Research
- Immune Response and Inflammation
- Fetal and Pediatric Neurological Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
Klinik für Schlafmedizin
2021-2023
University Hospital Münster
2021-2023
University of Münster
2023
Imaging Center
2021-2022
Heinrich Heine University Düsseldorf
2021
Background The inflammatory tumor microenvironment (TME) is formed by various immune cells, being closely associated with tumorigenesis. Especially, the interaction between tumor-infiltrating T-cells and macrophages has a crucial impact on progression metastatic spread. purpose of this study was to investigate whether oscillating-gradient diffusion-weighted MRI (OGSE-DWI) enables cell size-based discrimination different populations TME. Methods Sine-shaped OGSE-DWI combined Imaging...
Purpose To assess the feasibility of measuring tubular and vascular signal fractions in human kidney using nonnegative least‐square (NNLS) analysis intravoxel incoherent motion data collected healthy volunteers patients with renal pathologies. Methods MR imaging was performed at 3 Tesla 12 subjects various pathologies (fibrotic disease, failed graft, masses). Relative f mean diffusivities diffusion components cortex, medulla, lesions were obtained regularized NNLS fitting data. Test–retest...
Immune cells show distinct motion patterns that change upon inflammatory stimuli. Monocytes patrol the vasculature to screen for pathogens, thereby exerting an early task of innate immunity. Here, we aimed non-invasively analyse single patrolling monocyte behaviour stimuli.We used time-lapse Magnetic Resonance Imaging (MRI) murine brain dynamically track monocytes within circulation distant actual site inflammation in different conditions, ranging from a subcutaneous pellet model severe...
Temporal resolution of time-lapse MRI to track individual iron-labeled cells is limited by the required data-acquisition time fill k-space and reach sufficient SNR. Although motion slowly patrolling monocytes can be resolved, detection fast-moving immune requires improved acquisition reconstruction strategies.For accelerated cell tracking, a Cartesian sampling scheme was designed, in which fully sampled undersampled data for different acceleration factors were acquired simultaneously,...
Immune cells are major players of the tumor microenvironment (TME), having profound effects on development and metastatic progression. We present oscillating-gradient diffusion-weighted MRI (OGSE-DWI) as non-invasive imaging approach to monitor intratumoral immune cell infiltrate, relying size differences between cancer cells, T-cells macrophages. By applying Imaging Microstructural Parameters Using Limited Spectrally Edited Diffusion (IMPULSED) model sine-shaped OGSE-DWI, changes within TME...
Abstract Purpose Time‐lapse MRI enables tracking of single iron‐labeled cells. Yet, due to temporal blurring, only slowly moving cells can be resolved. To study faster for example during inflammatory processes, accelerated acquisition is needed. Methods A rotating phantom system was developed quantitatively measure the current maximum detectable speed in time‐lapse MRI. For cell tracking, an interleaved radial scheme applied and murine brain vivo experiments at 9.4 T. Detection evaluated...
Zielsetzung Immunzellen sind charakteristische Bestandteile des Tumormikromilieus (TME) und zentrale Mediatoren von Tumorprogression Metastasierung. dieser Studie ist die nicht-invasive in vivo Charakterisierung TME zur frühzeitigen Evaluation Therapieansprechen.
We validate that time-lapse MRI is able to resolve and follow single immune cells patrolling the brain vasculature. For reason, we simulate motion-dependent contrast based on artificial k-space, imitate migrating with a rotating phantom system, track iron-labeled monocytes in vivo repeated T2* weighted imaging. Furthermore, show allows differentiate between different motion patterns non-invasively whole-brain coverage, study altered behavior upon inflammatory stimulus onset of responses...
Zielsetzung Time-lapse MRT ermöglicht mittels repetitiver Bildakquisition die Darstellung einzelner Eisen-markierter Immunzellen im murinen Gehirn. Ziel dieses Projektes war eine Verbesserung der bisher zeitaufwändigen manuellen Datenauswertung. Zusätzlich wurde in vivo Zellgeschwindigkeits-Detektionsgrenze Phantommessungen validiert.
Zielsetzung Immunzellen zeigen ein typisches Bewegungsmuster, dass sich unter inflammatorischen Stimuli verändern kann. Bisher ist wenig über das sehr langsame, sogenannte patrolling der bekannt, da eine Einzelzellverfolgung bisher nur mit Intravitalmikroskopie möglich ist. Daher soll in dieser Arbeit Darstellung und Analyse patrollierender im Gesunden sowie Vorliegen verschiedener peripherer oder systemischer inflammatorischer mittels time-lapse MRT erfolgen.