A5031154029- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Gut microbiota and health
- Carbohydrate Chemistry and Synthesis
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Amoebic Infections and Treatments
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Parasitic Infections and Diagnostics
- HIV/AIDS drug development and treatment
- Influenza Virus Research Studies
- Animal Nutrition and Physiology
- Synthesis of heterocyclic compounds
- Microbial Natural Products and Biosynthesis
- Heparin-Induced Thrombocytopenia and Thrombosis
- Antimicrobial Resistance in Staphylococcus
- Platelet Disorders and Treatments
- Eicosanoids and Hypertension Pharmacology
- Bacterial biofilms and quorum sensing
- Bacillus and Francisella bacterial research
- Synthesis of Organic Compounds
- Synthesis and biological activity
Universität Greifswald
2016-2025
University of Rostock
1999-2007
Leibniz Institute for Catalysis
2007
The emergence of antibiotic resistant pathogenic bacteria has reduced our ability to combat infectious diseases. At the same time numbers new antibiotics reaching market have decreased. This situation created an urgent need discover novel scaffolds. Recently, application pattern recognition techniques identify molecular fingerprints in 'omics' studies, emerged as important tool biomedical research and laboratory medicine pathogens, monitor therapeutic treatments or develop drugs with...
TfCa, a promiscuous carboxylesterase from Thermobifida fusca, was found to hydrolyze polyethylene terephthalate (PET) degradation intermediates such as bis(2-hydroxyethyl) (BHET) and mono-(2-hydroxyethyl)-terephthalate (MHET). In this study, we elucidated the structures of TfCa in its apo form, well complex with PET monomer analogue BHET. The structure-function relationship investigated by comparing hydrolytic activity on various ortho- para-phthalate esters different lengths....
Abstract Background SARS-CoV-2 vaccine ChAdOx1 nCov-19 rarely causes vaccine-induced immune thrombotic thrombocytopenia (VITT) that—like autoimmune heparin-induced thrombocytopenia—is mediated by platelet-activating anti-platelet factor 4 (PF4) antibodies. Methods We investigated vaccine, PF4, and VITT patient-derived anti-PF4 antibody interactions using dynamic light scattering, 3D-super-resolution microscopy, electron microscopy. Mass spectrometry was used to analyze composition. the...
The successful sustainable cultivation of the well-known medicinal plant sundew on rewetted peatlands not only leads to preservation natural populations, but also provides a basis for pharmaceutical use plant. bioactive compounds sundew, flavonoids and naphthoquinones, show biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains open up new therapeutic possibilities. This study investigates molecular mechanisms these in biofilm inhibition through proteomic...
Abstract The cell wall synthesis pathway producing peptidoglycan is a highly coordinated and tightly regulated process. Although the major components of bacterial walls have been known for decades, complex regulatory network controlling many details division machinery are not well understood. eukaryotic-like serine/threonine kinase Stk cognate phosphatase Stp play an important role in biosynthesis drug resistance S. aureus . We show that stp deletion has pronounced impact on synthesis....
Entamoeba histolytica is an obligate protozoan parasite of humans, and amebiasis, infectious disease which targets the intestine and/or liver, second most common cause human death due to a after malaria. Although amebiasis usually asymptomatic, E. has potent pathogenic potential. During host infection, exposed reactive oxygen species that are produced released by cells innate immune system at site infection. The ability survive oxidative stress (OS) essential for successful invasion host....
Staphylococcus aureus is a major pathogen, which has to defend against reactive oxygen and electrophilic species encountered during infections. Activated macrophages produce the immunometabolite itaconate as potent electrophile antimicrobial upon pathogen infection. In this work, we used transcriptomics, metabolomics shotgun redox proteomics investigate specific stress responses, metabolic changes modifications caused by sublethal concentrations of itaconic acid in S. aureus. RNA-seq...
Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or licheniformis. In this study the response of to was investigated during treatment with high concentrations hydrogen peroxide at proteome, transcriptome and metabolome level. Genes/proteins belonging regulons, which are known have important functions in organisms, were found be upregulated, Fur, Spx, SOS CtsR regulon. Strikingly, parts...
Amebiasis, a global intestinal parasitic disease, is due to Entamoeba histolytica. This parasite, which feeds on bacteria in the large intestine of its human host, can trigger strong inflammatory response upon invasion colonic mucosa. Whereas information about mechanisms are used by parasite cope with oxidative and nitrosative stresses during infection available, knowledge contribution these lacking. In recent study, we demonstrated that enteropathogenic Escherichia coli O55 protects E....
To be a successful pathogen, Staphylococcus aureus has to adapt its metabolism the typically oxygen- and glucose-limited environment of host. Under fermenting conditions in presence glucose, S. uses glycolysis generate ATP via substrate-level phosphorylation mainly lactic acid fermentation maintain redox balance by reoxidation NADH equivalents. However, it is less clear how proceeds under anoxic glucose limitation, likely representing bona fide situation Using combination proteomic,...
The oxidative stress response of Bacillus licheniformis after treatment with hydrogen peroxide was investigated at the transcriptome, proteome and metabolome levels. In this comprehensive study, 84 proteins 467 transcripts were found to be up or downregulated in stressor. Among upregulated genes many that are known have important functions other organisms, such as catalase, alkylhydroperoxide reductase thioredoxin system. Many these could grouped into putative regulons by genomic mining....
Polyketides, such as erythromycin, are complex natural products with diverse therapeutic applications. They synthesized by multi-modular megaenzymes, so-called polyketide synthases (PKSs). The macrolide core of 6-deoxyerythronolide B (6dEB), is produced the deoxyerythronolide synthase (DEBS) that consists three proteins each a size 330–370 kDa. We cloned and investigated expression corresponding gene cluster from Saccharopolyspora erythraea, which comprises more than 30 kb, in Bacillus...
Seasonal influenza outbreaks represent a large burden for the health care system as well economy. While role of microbiome has been elucidated in context various diseases, impact respiratory viral infections on human is largely unknown. In this study, swine was used an animal model to characterize temporal dynamics and gastrointestinal response A virus (IAV) infection. multi-omics approach applied fecal samples identify alterations composition function during IAV We observed significantly...
The in vitro metabolism of flupirtine, ethyl-<i>N</i>-[2-amino-6-(4-fluorophenylmethyl-amino)pyridine-3-yl]carbamate, a centrally acting analgesic with muscle tone-reducing activity, was studied. Two flupirtine metabolites were already known: the <i>N</i>-acetylated analog D13223 and 4-fluorohippuric acid. structure suggested that redox chemistry may play role metabolism, cyclic voltammetry studies showed drug undergoes facile irreversible reactions. Thus, oxidative investigated first. With...
Aims The rare association of flupirtine with liver injury is most likely caused by reactive quinone diimines and their oxidative formation may be influenced the activities N ‐acetyltransferases ( NAT ) that conjugate less toxic metabolite D13223 , glucuronosyltransferases UGT glutathione S ‐transferases GST generate stable terminal glucuronides mercapturic acid derivatives, respectively. influence genetic polymorphisms NAT2 UGT1A1 GSTP1 on generation derivatives analgesic effects was...
Marine Bacteroidetes that degrade polysaccharides contribute to carbon cycling in the ocean. Organic matter, including glycans from terrestrial plants, might enter oceans through rivers. Whether marine bacteria structurally related diverse sources plants and algae was previously unknown. We show bacterium Flavimarina sp. Hel_I_48 encodes two polysaccharide utilization loci (PULs) which xylans algae. Biochemical experiments revealed activity specificity of encoded xylanases associated enzymes...
Abstract Adaptation of the Entamoeba histolytica parasite to toxic levels nitric oxide (NO) that are produced by phagocytes may be essential for establishment chronic amebiasis and parasite’s survival in its host. In order obtain insight into mechanism E. ’s adaptation NO, trophozoites were progressively adapted increasing concentrations NO donor drug, S-nitrosoglutathione (GSNO) up a concentration 110 μM. The transcriptome (NAT) was investigated RNA sequencing (RNA-seq). N-acetyl ornithine...
Swine are regarded as promising biomedical models, but the dynamics of their gastrointestinal microbiome have been much less investigated than that humans or mice. The aim this study was to establish an integrated multi-omics protocol investigate fecal healthy swine. To end, a preparation and analysis including sample for meta-omics analyses deep-frozen feces developed. Subsequent data integration linked composition with function, metabolic activity protein inventories, i.e., 16S rRNA...
A variety of 1,3,5-tricarbonyl derivatives were prepared by reaction 1,3-bis(silyl enol ethers) with acid chlorides under mild conditions. This includes reactions both aromatic and aliphatic bis(acid chlorides). The yields vary depending on the type chloride employed.
Abstract Background Bacterial protein biosynthesis usually depends on a formylated methionyl start tRNA but Staphylococcus aureus is viable in the absence of Fmt, Met formyl transferase. fmt mutants exhibit reduced growth rates indicating that function certain proteins N-termini it has remained unclear, which cellular processes are abrogated by lack formylation. Results In order to elucidate how global metabolic affected exometabolome an S. mutant was compared with parental strain and...