A5026679477- Microbial Community Ecology and Physiology
- Marine Biology and Ecology Research
- Genomics and Phylogenetic Studies
- Marine Bivalve and Aquaculture Studies
- Protist diversity and phylogeny
- Environmental DNA in Biodiversity Studies
- Botany and Plant Ecology Studies
- Ocean Acidification Effects and Responses
- Peatlands and Wetlands Ecology
- Advanced Proteomics Techniques and Applications
- Bacteriophages and microbial interactions
- Parasite Biology and Host Interactions
- Insect and Arachnid Ecology and Behavior
- bioluminescence and chemiluminescence research
- Coastal wetland ecosystem dynamics
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Physiological and biochemical adaptations
- Gut microbiota and health
- Isotope Analysis in Ecology
- Animal Behavior and Reproduction
- Bryophyte Studies and Records
- Aquatic Invertebrate Ecology and Behavior
- Marine and coastal ecosystems
- Antibiotic Resistance in Bacteria
Universität Greifswald
2014-2024
Ludwig-Maximilians-Universität München
2023
University of Calgary
2018-2021
Institute of Marine Biotechnology
2017-2021
Pharmaceutical Biotechnology (Czechia)
2017-2019
Universitätsmedizin Greifswald
2017-2018
Abstract Polysaccharide degradation by heterotrophic microbes is a key process within Earth’s carbon cycle. Here, we use environmental proteomics and metagenomics in combination with cultivation experiments biochemical characterizations to investigate the molecular details of situ polysaccharide mechanisms during microalgal blooms. For this, laminarin as model polysaccharide. Laminarin ubiquitous marine storage polymer microalgae particularly abundant phytoplankton In this study, show that...
Abstract Placozoa is an enigmatic phylum of simple, microscopic, marine metazoans 1,2 . Although intracellular bacteria have been found in all members this phylum, almost nothing known about their identity, location and interactions with host 3–6 We used metagenomic metatranscriptomic sequencing single individuals, plus metaproteomic imaging analyses, to show that the placozoan Trichoplax sp. H2 lives symbiosis two bacteria. One symbiont forms undescribed genus Midichloriaceae...
The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium its gills. endosymbiont fixes CO2 and thus generates organic carbon compounds, which support the host's growth. To investigate uncultured symbiont's metabolism symbiont-host interactions detail we conducted proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal hitherto completely unrecognized feature C. physiology: genome encodes all proteins necessary for...
Measurements of stable carbon isotope ratios (δ13C) are widely used in biology to address questions regarding food sources and metabolic pathways by organisms. The analysis these so-called fingerprints (SIFs) for microbes involved biogeochemical cycling microbiota plants animals has led major discoveries environmental microbiology. Currently, obtaining SIFs microbial communities is challenging as the available methods either only provide low taxonomic resolution, such use lipid biomarkers,...
Most autotrophs use the Calvin-Benson-Bassham (CBB) cycle for carbon fixation. In contrast, all currently described from Campylobacterota (previously Epsilonproteobacteria) reductive tricarboxylic acid (rTCA) instead. We discovered campylobacterotal epibionts ("Candidatus Thiobarba") of deep-sea mussels that have acquired a complete CBB and may lost most key genes rTCA cycle. Intriguingly, phylogenies suggest they were in multiple transfers Gammaproteobacteria closely related to...
All animals are associated with microorganisms; hence, host-microbe interactions of fundamental importance for life on earth. However, we know little about the molecular basis these interactions. Therefore, studied deep-sea Riftia pachyptila symbiosis, a model association in which tubeworm host is only one phylotype endosymbiotic bacteria and completely depends this sulfur-oxidizing symbiont nutrition. Using metaproteomics approach, identified both metabolic interaction processes, such as...
Metaproteomics, the study of protein expression in microbial communities, is a versatile tool for environmental microbiology. Achieving sufficiently high metaproteome coverage to obtain comprehensive picture activities and interactions communities one current challenges metaproteomics. An essential step maximize number identified proteins peptide separation via liquid chromatography (LC) prior mass spectrometry (MS). Thorough optimization comparison LC methods metaproteomics are, however,...
The hydrothermal vent tubeworm Riftia pachyptila hosts a single 16S rRNA phylotype of intracellular sulfur-oxidizing symbionts, which vary considerably in cell morphology and exhibit remarkable degree physiological diversity redundancy, even the same host. To elucidate whether multiple metabolic routes are employed cells or rather distinct symbiont subpopulations, we enriched symbionts according to size by density gradient centrifugation. Metaproteomic analysis, microscopy, flow cytometry...
Abstract Deep-sea Bathymodiolus mussels and their chemoautotrophic symbionts are well-studied representatives of mutualistic host–microbe associations. However, how host–symbiont interactions vary on the molecular level between related host symbiont species remains unclear. Therefore, we compared metaproteomes Pacific B. thermophilus, hosting a thiotrophic symbiont, Atlantic azoricus, containing two symbionts, thiotroph methanotroph. We identified common strategies metabolic support hosts...
Abstract Sexual dimorphism arises because of divergent fitness optima between the sexes. Phenotypic divergence sexes can range from mild to extreme. Fireflies, bioluminescent beetles, present various degrees sexual dimorphism, with species showing very presenting female-specific neoteny, posing a unique framework investigate evolution sexually dimorphic traits across species. In this work, we novel assembled genomes two firefly species, Lamprohiza splendidula and Luciola italica , different...
Seasonal influenza outbreaks represent a large burden for the health care system as well economy. While role of microbiome has been elucidated in context various diseases, impact respiratory viral infections on human is largely unknown. In this study, swine was used an animal model to characterize temporal dynamics and gastrointestinal response A virus (IAV) infection. multi-omics approach applied fecal samples identify alterations composition function during IAV We observed significantly...
Abstract The ability to buffer detrimental effects of environmental stress on fitness is great ecological importance because, in nature, pronounced variation may regularly induce stress. Furthermore, several stressors interact a synergistic manner. In the present study, plastic responses cold, heat and starvation resistance are investigated tropical butterfly Bicyclus anynana Butler, 1879, using full factorial design with two acclimation temperatures (20 27 °C) four short‐term treatments...
Abstract Peat formation is a key carbon sequestration process in the terrestrial biosphere. In temperate fens, peat mainly formed by below‐ground biomass of vascular plants. Nutrient availability fens naturally variable, and nowadays increasing due to atmospheric deposition, runoff from agriculture mineralization caused drainage. To maintain or restore formation, it important understand how increased nutrient influences main controls that is, production decomposition. We investigated above‐...
Ecosystems subject to mantle degassing are of particular interest for understanding global biogeochemistry, as their microbiomes shaped by prolonged exposure high CO
Abstract As one of the most-consumed drugs worldwide, ibuprofen (IBU) reaches environment in considerable amounts as environmental pollutant, necessitating studies its further biotransformation potential removal mechanism. Therefore, we screened bacteria with known capabilities to degrade aromatic pollutants, belonging genera Bacillus , Priestia (formerly also ) Paenibacillus Mycobacterium and Cupriavidus for their ability transform ibuprofen. We identified five transformation products,...
Abstract Although the majority of known autotrophs use Calvin-Benson-Bassham (CBB) cycle for carbon fixation, all currently described from Campylobacterota (previously Epsilonproteobacteria) reductive tricarboxylic acid (rTCA) instead. We discovered campylobacterotal epibionts (“ Candidatus Thiobarba”) deep-sea mussels that have acquired a complete CBB and lost key genes rTCA cycle. Intriguingly, phylogenies suggest they were in multiple transfers Gammaproteobacteria closely related to...
This protocol describes an optimized protein extraction method for (meta-) proteomic analyses. It is based on several existing protocols (see references below) that were combined and adapted also compatible with low biomass samples. References: Erde et al. 2014, J. Proteome Res. 13, 1885-1895 Kleiner 2017, Nat. Comm. 8, Article Number 1558 Kulak Methods 11, 319-324 León 2013, Mol. Cell. Proteomics 12, 2992-3005 Masuda 2008, 7, 731-740 Wang 2015, Int. Anal. Chem. 2015,Article ID 763969...
Abstract Measurements of the carbon stable isotope ratio (δ 13 C) are widely used in biology to address major questions regarding food sources and metabolic pathways by organisms. Measurement these so called fingerprints (SIFs) for microbes involved biogeochemical cycling microbiota plants animals have led discoveries environmental microbiology. Currently, obtaining SIFs microbial communities is challenging as available methods either only provide limited taxonomic resolution, such with use...
Abstract In wet peatlands, plant growth conditions are largely determined by local soil conditions, leading to locally adapted vegetation. Despite that Carex species often the prevailing vascular in fen peatlands of temperate zone, information about how these adapt environmental is scarce. This holds true especially for below-ground traits and adaptations fen-typical nutrient level variations. To address this research gap, we investigated different geographic origins (Germany, Poland, The...