A5077814158- Genetics, Aging, and Longevity in Model Organisms
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Marine Biology and Ecology Research
- Microbial Community Ecology and Physiology
- Marine Bivalve and Aquaculture Studies
- Tryptophan and brain disorders
- Evolution and Genetic Dynamics
- Parasite Biology and Host Interactions
- Parasitic Diseases Research and Treatment
- Parasitic Infections and Diagnostics
- Circadian rhythm and melatonin
- Phagocytosis and Immune Regulation
- Distributed systems and fault tolerance
- Gastrointestinal motility and disorders
- Nanoparticles: synthesis and applications
- Pancreatitis Pathology and Treatment
- Biomedical Ethics and Regulation
- Immune cells in cancer
- Bacteriophages and microbial interactions
- Diverse Legal and Medical Studies
- Food composition and properties
- Parasites and Host Interactions
- Protist diversity and phylogeny
- Nematode management and characterization studies
Baylor College of Medicine
2018-2024
Max Planck Institute for Marine Microbiology
2016-2024
The study of microbiomes by sequencing has revealed a plethora correlations between microbial community composition and various life-history characteristics the corresponding host species. However, inferring causation from correlation is often hampered sheer compositional complexity microbiomes, even in simple organisms. Synthetic communities offer an effective approach to infer cause-effect relationships host-microbiome systems. Yet available suffer several drawbacks, such as artificial...
Host genetic landscapes can shape microbiome assembly in the animal gut by contributing to establishment of distinct physiological environments. However, determinants stability and variation these types remain largely undefined. Here, we use free-living nematode Caenorhabditis elegans identify natural among wild strains C. that drives microbiomes. To achieve this, first established a diverse model represents strain-level phylogenetic diversity naturally encountered wild. Using this...
Most autotrophs use the Calvin-Benson-Bassham (CBB) cycle for carbon fixation. In contrast, all currently described from Campylobacterota (previously Epsilonproteobacteria) reductive tricarboxylic acid (rTCA) instead. We discovered campylobacterotal epibionts ("Candidatus Thiobarba") of deep-sea mussels that have acquired a complete CBB and may lost most key genes rTCA cycle. Intriguingly, phylogenies suggest they were in multiple transfers Gammaproteobacteria closely related to...
The gut microbiota is essential for maintenance and repair of the intestinal epithelial barrier. As shifts in both barrier function composition are found inflammatory bowel disease patients, it critical to understand role distinct bacteria regulating repair. We identified a mouse commensal E. coli isolate, GDAR2-2, that protects mice from Citrobacter rodentium infection dextran sulfate sodium-induced colitis. Colonization with GDAR2-2 resulted expansion CX3CR1+ mononuclear phagocytes,...
Bathymodiolus mussels dominate animal communities at many hydrothermal vents and cold seeps. Essential to the mussels' ecological evolutionary success is their association with symbiotic methane- sulfur-oxidizing gammaproteobacteria, which provide them nutrition. In addition these well-known gammaproteobacterial endosymbionts, we found epsilonproteobacterial sequences in metatranscriptomes, metagenomes 16S rRNA clone libraries as well by polymerase chain reaction screening of species sampled...
Bacterial nanocellulose (BNC) is a promising dietary fiber with potential as functional food additive. We evaluated BNC fibers (BNCf) in the Caenorhabditis elegans model to obtain insight into BNCf's biointeraction its gastrointestinal tract while reducing variables of higher complex animals. BNCf were uptaken and excreted by worms without crossing intestinal barrier, confirming biosafety regarding survival rate, reproduction, aging for concentrations up 34 μg/ml BNCf. However, slight...
Chronic exposure to high-fat diets (HFD) worsens intestinal disease pathology, but acute effects of HFD in tissue damage remain unclear. Here, we used short-term feeding a model injury and found sustained with increased cecal dead neutrophil accumulation, along dietary lipid accumulation. Neutrophil depletion rescued enhanced pathology. Macrophages from HFD-treated mice showed reduced capacity engulf neutrophils. Macrophage clearance neutrophils activates critical barrier repair...
Abstract Background Skin-penetrating nematodes of the genus Strongyloides infect over 600 million people, posing a major global health burden. Their life cycle includes both parasitic and free-living generation. During generation, infective third-stage larvae (iL3s) actively engage in host seeking. develop reproduce on feces. At different points during their cycle, species encounter wide variety host-associated environmental bacteria. However, microbiome associated with species, behavioral...
Hydrogen peroxide (H 2 O ) is the most common chemical threat that organisms face. Here, we show H alters bacterial food preference of Caenorhabditis elegans , enabling nematodes to find a safe environment with food. induces leave patches laboratory and microbiome bacteria when those communities have insufficient -degrading capacity. The nematode’s behavior directed by -sensing neurons promote escape from bacteria-sensing attraction bacteria. However, input for removed enzymes neurons’...
Abstract Endozoicomonadaceae bacteria are widespread in many marine animals, and generally considered beneficial. Members of one clade, however, Candidatus Endonucleobacter, infect the nuclei deep-sea mussels, where they replicate to ≥ 80,000 per nucleus cause swell 50 times their original size. How these parasites able persist host without cell undergoing apoptosis is not known. We show here that Ca. Endonucleobacter encodes expresses 7-15 inhibitors (IAPs), proteins previously only known...
Abstract Although the majority of known autotrophs use Calvin-Benson-Bassham (CBB) cycle for carbon fixation, all currently described from Campylobacterota (previously Epsilonproteobacteria) reductive tricarboxylic acid (rTCA) instead. We discovered campylobacterotal epibionts (“ Candidatus Thiobarba”) deep-sea mussels that have acquired a complete CBB and lost key genes rTCA cycle. Intriguingly, phylogenies suggest they were in multiple transfers Gammaproteobacteria closely related to...
ABSTRACT The study of microbiomes by sequencing has revealed a plethora correlations between microbial community composition and various life-history characteristics the corresponding host species. However, inferring causation from correlation is often hampered sheer compositional complexity microbiomes, even in simple organisms. Synthetic communities offer an effective approach to infer cause-effect relationships host-microbiome systems. Yet available suffer several drawbacks, such as...
Summary Hydrogen peroxide (H 2 O ) is the most common chemical threat that organisms face. Here, we show H alters bacterial food preference of Caenorhabditis elegans , enabling nematodes to find a safe environment with food. induces leave patches laboratory and microbiome bacteria when those communities have insufficient -degrading capacity. The nematode’s behavior directed by -sensing neurons promote escape from bacteria-sensing attraction bacteria. However, input for removed enzymes...
The composition of the gut microbiome can have a dramatic impact on host physiology throughout development and life animal. Measuring compositional changes in is crucial identifying functional relationships between these physiological changes. Caenorhabditis elegans has emerged as powerful system to examine molecular drivers host-microbiome interactions. With its transparent body plan fluorescent-tagged natural microbes, relative levels microbes within an individual C. animal be easily...
The composition of the gut microbiome can have a dramatic impact on host physiology throughout development and life animal. Measuring compositional changes in is crucial identifying functional relationships between these physiological changes. Caenorhabditis elegans has emerged as powerful system to examine molecular drivers host-microbiome interactions. With its transparent body plan fluorescent-tagged natural microbes, relative levels microbes within an individual C. animal be easily...
The gut microbiome is an important driver of host physiology and development. Altered abundance or membership this microbe community can influence health disease progression, including the determination lifespan healthspan. Here, we describe a robust pipeline to measure composition in C. elegans that be applied examine role on aging processes.
Here is a short explanation on how to add work in progress protocol private group. This shows editable by multiple people edit it before its final publication protocols.io also an example explain the members of group share protocols.
NGM agar recipe from wormbook
M9 Buffer recipe, transposed from the WormBook chapter: Maintenance ofC. elegans https://www.ncbi.nlm.nih.gov/books/NBK19649/
Worm Freezing buffer recipe according to Brenner, S. (1974). Genetics77, 71. Found on wormbook see external link