A5057404480- Marine Biology and Ecology Research
- Parasite Biology and Host Interactions
- Microbial Community Ecology and Physiology
- Botany and Plant Ecology Studies
- Insect symbiosis and bacterial influences
- Marine Bivalve and Aquaculture Studies
- Insects and Parasite Interactions
- Leech Biology and Applications
- Nematode management and characterization studies
- Protist diversity and phylogeny
- Cephalopods and Marine Biology
- Entomological Studies and Ecology
- Mediterranean and Iberian flora and fauna
- Genomics and Phylogenetic Studies
- Animal Ecology and Behavior Studies
- Plant and animal studies
- Aquatic Invertebrate Ecology and Behavior
- Planarian Biology and Electrostimulation
- Coral and Marine Ecosystems Studies
- Coleoptera Taxonomy and Distribution
- Bacteriophages and microbial interactions
- Marine and coastal plant biology
- Botanical Studies and Applications
- Vibrio bacteria research studies
- Marine Ecology and Invasive Species
Max Planck Institute for Marine Microbiology
2016-2024
European Molecular Biology Laboratory
2024
Max Planck Society
2016-2019
University of Vienna
2011-2018
A mesophilic, neutrophilic and aerobic, ammonia-oxidizing archaeon, strain EN76 T , was isolated from garden soil in Vienna (Austria). Cells were irregular cocci with a diameter of 0.6–0.9 µm possessed archaella archaeal pili as cell appendages. Electron microscopy also indicated clearly discernible areas high low electron density, well tubule-like structures. Strain had an S-layer p3 symmetry, so far only reported for members the Sulfolobales . Crenarchaeol major core lipid. The organism...
ABSTRACT Nitrification is a key process of the biogeochemical nitrogen cycle and biological wastewater treatment. The second step, nitrite oxidation to nitrate, catalyzed by phylogenetically diverse, chemolithoautotrophic nitrite-oxidizing bacteria (NOB). Uncultured NOB from genus “ Candidatus Nitrotoga” are widespread in natural engineered ecosystems. Knowledge about their biology sparse, because no genomic information pure Ca . culture was available. Here we obtained first isolate...
Abstract Symbiotic N 2 -fixing microorganisms have a crucial role in the assimilation of nitrogen by eukaryotes nitrogen-limited environments 1–3 . Particularly among land plants, symbionts occur variety distantly related plant lineages and often involve an intimate association between host symbiont 2,4 Descriptions such symbioses are lacking for seagrasses, which evolved around 100 million years ago from terrestrial flowering plants that migrated back to sea 5 Here we describe symbiont, ‘...
Harnessing chemosynthetic symbionts is a recurring evolutionary strategy. Eukaryotes from six phyla as well one archaeon have acquired chemoautotrophic sulfur-oxidizing bacteria. In contrast to this broad host diversity, known bacterial partners apparently belong two classes of bacteria—the Gamma - and Epsilonproteobacteria . Here, we characterize the intracellular endosymbionts mouthless catenulid flatworm genus Paracatenula Alphaproteobacteria The galateia are provisionally classified “...
Abstract Placozoa is an enigmatic phylum of simple, microscopic, marine metazoans 1,2 . Although intracellular bacteria have been found in all members this phylum, almost nothing known about their identity, location and interactions with host 3–6 We used metagenomic metatranscriptomic sequencing single individuals, plus metaproteomic imaging analyses, to show that the placozoan Trichoplax sp. H2 lives symbiosis two bacteria. One symbiont forms undescribed genus Midichloriaceae...
Most autotrophs use the Calvin-Benson-Bassham (CBB) cycle for carbon fixation. In contrast, all currently described from Campylobacterota (previously Epsilonproteobacteria) reductive tricarboxylic acid (rTCA) instead. We discovered campylobacterotal epibionts ("Candidatus Thiobarba") of deep-sea mussels that have acquired a complete CBB and may lost most key genes rTCA cycle. Intriguingly, phylogenies suggest they were in multiple transfers Gammaproteobacteria closely related to...
Hosts of chemoautotrophic bacteria typically have much higher biomass than their symbionts and consume symbiont cells for nutrition. In contrast to this, Candidatus Riegeria in mouthless Paracatenula flatworms comprise up half the consortium. Each species harbors a specific Ca Riegeria, endosymbionts been vertically transmitted at least 500 million years. Such prolonged strict vertical transmission leads streamlining genomes, retained physiological capacities reveal functions provide hosts....
How and when symbionts are acquired by their animal hosts has a profound impact on the ecology evolution of symbiosis. Understanding symbiont acquisition is particularly challenging in deep-sea organisms because early life stages so rarely found. Here, we collected developmental three bathymodioline species from different habitats to identify these acquire how body plan adapts symbiotic lifestyle. These mussels gain nutrition chemosynthetic bacteria, allowing them thrive at vents seeps...
Rickettsiales are an enigmatic clade of strictly host-associated bacteria, which some very common in aquatic habitats. Despite a wealth sequencing-based observations, the hosts for many these marine still unknown. The first molecular data from two closely related placozoan species points to as abundant symbionts simple and early-branching animals. Given electron microscopy observations intracellular Rickettsia-like-organisms all placozoans examined, we systematically characterized...
Bathymodiolus mussels dominate animal communities at many hydrothermal vents and cold seeps. Essential to the mussels' ecological evolutionary success is their association with symbiotic methane- sulfur-oxidizing gammaproteobacteria, which provide them nutrition. In addition these well-known gammaproteobacterial endosymbionts, we found epsilonproteobacterial sequences in metatranscriptomes, metagenomes 16S rRNA clone libraries as well by polymerase chain reaction screening of species sampled...
Many marine biology studies depend on field work ships or remote sampling locations where sophisticated sample preservation techniques (e.g., high-pressure freezing) are often limited unavailable. Our aim was to optimize the ultrastructural of invertebrates, especially when working in field. To achieve chemically-fixed material highest quality, we compared resulting ultrastructure gill tissue mussel Mytilus edulis fixed with differently buffered EM fixatives for specimens (seawater,...
Abstract: To target chronic inflammatory ocular surface diseases, a drug delivery platform is needed that safe, possesses immunomodulatory properties, and can be used either for delivery, or as foreign antigen carrier. A new therapeutic approach we have previously proposed uses nonliving bacterial ghosts (BGs) carrier-delivery system which engineered to carry antigens and/or loaded with drugs. The parent strain chosen development of our BG the probiotic Escherichia coli Nissle 1917 (EcN),...
Large mussels of the mytilid subfamily Bathymodiolinae are common inhabitants deep-sea hydrothermal vents and cold seeps, where gill-borne symbionts allow them to utilize energy-rich compounds such as hydrogen sulfide methane support abundant growth. This also includes smaller symbiont-bearing found on wood organic deposits. Phylogenetic analyses suggest that association is ancestral bathymodiolin evolution. observation led "wooden steps" hypothesis, which proposed other large deposits have...
Abstract Microbial eukaryotes are small and often resistant to standard labelling imaging techniques, therefore remain understudied – despite their critical ecological importance - with the exception of a few established models. Here, we use Ultrastructure Expansion Microscopy (U-ExM) carry out high-resolution volumetric over 200 cultured planktonic across major lineages. By combining U-ExM pan- specific immuno-labelling, reveal novel microtubule centrin-containing elements assign molecular...
Bacteriocytes set the stage for some of most intimate interactions between animal and bacterial cells. In all bacteriocyte possessing systems studied so far, de novo formation bacteriocytes occurs only once in host development, at time symbiosis establishment. Here, we present free-living symbiotic flatworm Paracatenula galateia its intracellular, sulfur-oxidizing bacteria as a system with previously undescribed strategies symbiont transmission. Using thymidine analogue S-phase labeling...
Abstract Two long-standing paradigms in biology are that cells belonging to the same population exhibit little deviation from their average size and symmetric cell division is limited. Here, ultrastructural, morphometric immunocytochemical analyses reveal two Gammaproteobacteria attached cuticle of marine nematodes Eubostrichus fertilis E. dianeae reproduce by constricting a single FtsZ ring at midcell despite being 45 μm 120 long, respectively. In crescent-shaped bacteria coating ,...
Barnacles produce a proteinaceous adhesive called cement to attach permanently rocks or other hard substrata. The stalked barnacle Dosima fascicularis is of special interest as it produces large amount foam-like that can be used float. morphology the apparatus and polymerized this species almost unknown. current study aims at filling these gaps in our knowledge using light electron microscopy well x-ray microtomography. shape gland cells changes from round ovoid during development. cytoplasm...
Abstract Nematodes are one of the most widespread and abundant animal taxa across aquatic terrestrial environments. In marine shallow-water, porous sediments, members subfamily Stilbonematinae may be found in high numbers. characterized by their coat symbiotic bacteria, which give nematodes a white appearance, while themselves usually colourless. We identified several species genus Robbea (Desmodoridae: Stilbonematinae) live specimens had conspicuous dark purple coloration glandular sense...
Marine catenulid platyhelminths of the genus Paracatenula lack mouth, pharynx and gut. They live in a symbiosis with intracellular bacteria which are restricted to body region posterior brain. The symbiont-housing cells (bacteriocytes) collectively form trophosome tissue, functionally replaces digestive tract. It constitutes largest part is most important synapomorphy this group. While some other features anatomy have already been analyzed, an in-depth analysis was missing. Here, we identify...
Abstract Paracatenula galateia sp. nov. is a mouthless marine catenulid platyhelminth with bacterial intracellular endosymbionts. The worms live in shallow back-reef sands the Belize Barrier Reef system and are distinguished from four previously described members of genus by their large size combined ribbon-shaped body characteristic bipartite inclusions cells, which interpreted as sperm. bacteria presumed to be sulphur-oxidizing chemoautotrophs. They found bacteriocytes fill region...