A5056228868- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Wastewater Treatment and Nitrogen Removal
- Marine Biology and Ecology Research
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Environmental DNA in Biodiversity Studies
- Gut microbiota and health
- Polar Research and Ecology
- Isotope Analysis in Ecology
- RNA modifications and cancer
- Marine Bivalve and Aquaculture Studies
- Corporate Governance and Management
- Diatoms and Algae Research
- Marine and coastal plant biology
- Biosensors and Analytical Detection
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- Physics and Engineering Research Articles
- Microbial Fuel Cells and Bioremediation
- Gene expression and cancer classification
Max Planck Institute for Marine Microbiology
2016-2025
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
2021
Serviceplan (Germany)
2014-2019
Max Planck Society
2003-2017
GFZ Helmholtz Centre for Geosciences
2014
Technical University of Munich
1999-2007
Wienerberger (Czechia)
2007
Mediacult
2007
University of Music and Performing Arts Vienna
2007
University of Vienna
2007
Sequencing ribosomal RNA (rRNA) genes is currently the method of choice for phylogenetic reconstruction, nucleic acid based detection and quantification microbial diversity. The ARB software suite with its corresponding rRNA datasets has been accepted by researchers worldwide as a standard tool large scale analysis. However, rapid increase publicly available sequence data recently hampered maintenance comprehensive curated knowledge databases. A new system, SILVA (from Latin silva , forest),...
Phytoplankton blooms characterize temperate ocean margin zones in spring. We investigated the bacterioplankton response to a diatom bloom North Sea and observed dynamic succession of populations at genus-level resolution. Taxonomically distinct expressions carbohydrate-active enzymes (transporters; particular, TonB-dependent transporters) phosphate acquisition strategies were found, indicating that Bacteroidetes, Gammaproteobacteria, Alphaproteobacteria are specialized for successive...
ABSTRACT Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to investigate the phylogenetic composition of bacterioplankton communities several freshwater and marine samples. An average about 50% cells were detected by for domains Bacteria Archaea , these, half could be identified at subdomain level a set group-specific probes. Beta subclass proteobacteria constituted dominant fraction systems, accounting 16% (range, 3 32%) cells, although they...
In many oceanic regions, growth of phytoplankton is nitrogen-limited because fixation N 2 cannot make up for the removal fixed inorganic nitrogen (NH + 4 , NO - and 3 ) by anaerobic microbial processes. Globally, 30-50% total loss occurs in oxygen-minimum zones (OMZs) commonly attributed to denitrification (reduction nitrate heterotrophic bacteria). Here, we show that instead, anammox process (the oxidation ammonium nitrite yield mainly responsible OMZ waters one most productive regions...
A process of global importance in carbon cycling is the remineralization algae biomass by heterotrophic bacteria, most notably during massive marine blooms. Such blooms can trigger secondary planktonic bacteria that consist swift successions distinct bacterial clades, prominently members Flavobacteriia, Gammaproteobacteria and alphaproteobacterial Roseobacter clade. We investigated such spring phytoplankton southern North Sea (German Bight) for four consecutive years. Dense sampling...
ABSTRACT In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited low signal intensities. addition to an impermeability the cell periphery and a cellular rRNA content, three-dimensional structure ribosome may hinder access oligonucleotides their target sites. Until now, systematic study on accessibility 16S sites had not been done. Here, we report fluorescence intensities obtained more than 200...
ABSTRACT Submarine mud volcanoes are formed by expulsions of mud, fluids, and gases from deeply buried subsurface sources. They highly reduced benthic habitats often associated with intensive methane seepage. In this study, the microbial diversity community structure in methane-rich sediments Haakon Mosby Mud Volcano (HMMV) were investigated comparative sequence analysis 16S rRNA genes fluorescence situ hybridization. active volcano center, which has a diameter about 500 m, main...
Abstract Marine algae annually sequester petagrams of carbon dioxide into polysaccharides, which are a central metabolic fuel for marine cycling. Diatom microalgae produce sulfated polysaccharides containing methyl pentoses that challenging to degrade bacteria compared other monomers, implicating these sugars as potential sink. Free-living occurring in phytoplankton blooms specialise on consuming microalgal sugars, fucose and rhamnose remain unknown. Here, genomic proteomic data indicate...
Abstract Background Blooms of marine microalgae play a pivotal role in global carbon cycling. Such blooms entail successive specialized clades planktonic bacteria that collectively remineralize gigatons algal biomass on scale. This is largely composed distinct polysaccharides, and the microbial decomposition these polysaccharides therefore process prime importance. Results In 2020, we sampled complete biphasic spring bloom German Bight over 90-day period. Bacterioplankton metagenomes from 30...
Abstract Background Marine microalgae (phytoplankton) mediate almost half of the worldwide photosynthetic carbon dioxide fixation and therefore play a pivotal role in global cycling, most prominently during massive phytoplankton blooms. Phytoplankton biomass consists considerable proportions polysaccharides, substantial parts which are rapidly remineralized by heterotrophic bacteria. We analyzed diversity, activity, functional potential such polysaccharide-degrading bacteria different size...
ABSTRACT Target site inaccessibility represents a significant problem for fluorescence in situ hybridization (FISH) of 16S rRNA with oligonucleotide probes. Here, unlabeled oligonucleotides (helpers) that bind adjacent to the probe target were evaluated their potential increase weak signals Escherichia coli DSM 30083 T . The use helpers enhanced signal all six probes examined at least fourfold. In one case, Eco474 was increased 25-fold single helper probe, H440-2. another four raised FISH...
ABSTRACT Direct evidence that marine cyanobacteria take up organic nitrogen compounds in situ at high rates is reported. About 33% of the total bacterioplankton turnover amino acids, determined with a representative [ 35 S]methionine precursor and flow sorting, can be assigned to Prochlorococcus spp. 3% Synechococcus oligotrophic mesotrophic parts Arabian Sea, respectively. This finding may provide mechanism for ' competitive dominance over both strictly autotrophic algae other bacteria...
The anaerobic oxidation of ammonium (anammox) contributes significantly to the global loss fixed nitrogen and is carried out by a deep branching monophyletic group bacteria within phylum Planctomycetes. Various studies have implicated anammox be most important process responsible for in marine oxygen minimum zones (OMZs) with low diversity bacteria. This comprehensive study investigated suboxic zone Black Sea three major OMZs (off Namibia, Peru Arabian Sea). population composition were both,...
The substrate fluorescein-tyramide was combined with oligonucleotide probes directly labeled horseradish peroxidase to improve the sensitivity of in situ hybridization whole fixed bacterial cells. Flow cytometry and quantitative microscopy cells hybridized by this technique showed 10- 20-fold signal amplifications relative fluorescein-monolabeled probes. application new detection natural communities resulted very bright signals; however, number detected significantly lower than that...
The algal osmolyte, dimethylsulphoniopropionate (DMSP), is abundant in the surface oceans and major precursor of dimethyl sulphide (DMS), a gas involved global climate regulation. Here, we report results from an situ Lagrangian study that suggests link between microbially driven fluxes dissolved DMSP (DMSPd) specific members bacterioplankton community North Sea coccolithophore bloom. bacterial population bloom was dominated by single species related to genus Roseobacter , which accounted for...
Summary Members of the prokaryotic picoplankton are main drivers biogeochemical cycles over large areas world's oceans. In order to ascertain changes in composition euphotic and twilight zones at an ocean basin scale we determined distribution 11 marine bacterial archaeal phyla three different water layers along a transect across Atlantic Ocean from South Africa (32.9°S) UK (46.4°N) during boreal spring. Depth profiles down 500 m 65 stations were analysed by catalysed reporter deposition...
Recent studies have shown that the anaerobic oxidation of ammonium by anammox bacteria plays an important role in catalyzing loss nitrogen from marine oxygen minimum zones (OMZ). However, situ concentrations up to 25 microM and close or below detection limit layer activity are hard reconcile with current knowledge physiology bacteria. We therefore investigated samples Namibian OMZ comparative 16S rRNA gene analysis fluorescence hybridization. Our results showed "Candidatus Scalindua" spp.,...
ABSTRACT A flow-sorting technique was developed to determine unperturbed metabolic activities of phylogenetically characterized bacterioplankton groups with incorporation rates [ 35 S]methionine tracer. According fluorescence in situ hybridization rRNA targeted oligonucleotide probes, a clade α-proteobacteria, related Roseobacter spp., and Cytophaga-Flavobacterium cluster dominated the different groups. Cytometric characterization revealed both these have high DNA (HNA) content, while...
Heterotrophic microbial communities process much of the carbon fixed by phytoplankton in ocean, thus having a critical role global cycle. A major fraction phytoplankton-derived substrates are high-molecular-weight (HMW) polysaccharides. For bacterial uptake, these must initially be hydrolysed to smaller sizes extracellular enzymes. We investigated polysaccharide hydrolysis during transect Atlantic Ocean, and serendipitously discovered-using super-resolution structured illumination...