A5035917383- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Methane Hydrates and Related Phenomena
- Marine Biology and Ecology Research
- Genomics and Phylogenetic Studies
- Marine Bivalve and Aquaculture Studies
- Food Industry and Aquatic Biology
- Ocean Acidification Effects and Responses
- Oil Spill Detection and Mitigation
- Microplastics and Plastic Pollution
- Wastewater Treatment and Nitrogen Removal
- Polar Research and Ecology
- Enzyme Production and Characterization
- Microbial bioremediation and biosurfactants
- Ruminant Nutrition and Digestive Physiology
- Atmospheric and Environmental Gas Dynamics
- Hydrocarbon exploration and reservoir analysis
- Protist diversity and phylogeny
- Metabolomics and Mass Spectrometry Studies
- Arctic and Antarctic ice dynamics
- Geochemistry and Elemental Analysis
- Geological Studies and Exploration
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Geology and Paleoclimatology Research
University of North Carolina at Chapel Hill
2016-2025
University of North Carolina Health Care
2025
Max Planck Institute for Marine Microbiology
1996-2024
North Carolina State University
2020
Max Planck Society
1995-2018
University of Southern Denmark
2008
Dalhousie University
2008
Fahrenheit (Germany)
1995
Woods Hole Oceanographic Institution
1994
Forschungszentrum Jülich
1987
Microbial hydrolysis of polysaccharides is critical to ecosystem functioning and great interest in diverse biotechnological applications, such as biofuel production bioremediation. Here we demonstrate the use a new, efficient approach recover genomes active polysaccharide degraders from natural, complex microbial assemblages, using combination fluorescently labeled substrates, fluorescence-activated cell sorting, single genomics. We employed this analyze freshwater coastal bacterioplankton...
ABSTRACT In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys water column sediments. an fjord (Smeerenburgfjord) Svalbard, , together with Flavobacteria smaller proportions Alpha - Gammaproteobacteria constituted most frequently detected bacterioplankton community gene-based library analyses column. Parallel measurements activities six endo-acting...
Microorganisms have been repeatedly discovered in environments that do not support their metabolic activity. Identifying and quantifying these misplaced organisms can reveal dispersal mechanisms shape natural microbial diversity. Using endospore germination experiments, we estimated a stable supply of thermophilic bacteria into permanently cold Arctic marine sediment at rate exceeding 10(8) spores per square meter year. These metabolically phylogenetically diverse Firmicutes show no...
Heterotrophic microbial communities process much of the carbon fixed by phytoplankton in ocean, thus having a critical role global cycle. A major fraction phytoplankton-derived substrates are high-molecular-weight (HMW) polysaccharides. For bacterial uptake, these must initially be hydrolysed to smaller sizes extracellular enzymes. We investigated polysaccharide hydrolysis during transect Atlantic Ocean, and serendipitously discovered-using super-resolution structured illumination...
Abstract Identifying the roles played by individual heterotrophic bacteria in degradation of high molecular weight (HMW) substrates is critical to understanding constraints on carbon cycling ocean. At five sites Atlantic Ocean, we investigated processing organic matter tracking changes microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time. During this investigation, discovered that a considerable fraction uses newly-identified ‘selfish’ mode substrate...
The dynamics of the particulate organic carbon (POC) pool in ocean are central to marine cycle. POC is link between surface primary production, deep ocean, and sediments. rate at which degraded dark can impact atmospheric CO2 concentration. Therefore, a focus geochemistry studies improve our understanding distribution, composition, cycling. last few decades have seen improvements analytical techniques that greatly expanded what we measure, both terms compound structural diversity isotopic...
Heterotrophic microbial communities in seawater and sediments metabolize much of the organic carbon produced ocean. Although cycling preservation depend critically on capabilities these communities, their compositions have seldom been examined simultaneously at same site. To compare abilities sedimentary to initiate matter degradation, we measured extracellular enzymatic hydrolysis rates 10 substrates (polysaccharides algal extracts) surface bottom water as well anoxic an Arctic fjord....
The Deepwater Horizon oil spill triggered a complex cascade of microbial responses that reshaped the dynamics heterotrophic carbon degradation and turnover dissolved organic (DOC) in contaminated waters. Our results from 21-day laboratory incubations rotating glass bottles (roller bottles) demonstrate flux oil-contaminated surface water sampled near site two weeks after onset blowout were greatly affected by activities microbes associated with macroscopic aggregates. Roller oil-amended...
Abstract High hydrostatic pressure is characteristic of the deep ocean and presumed to influence microbial functions viability. However, marine processes are typically measured only at atmospheric (0.1 MPa), limiting our understanding effects on activities microbes that sink as part biological carbon pump well those reside in ocean. To test functions, we extracellular enzymatic activities—the first step organic matter remineralization—of a moderate piezophile (Photobacterium profundum SS9),...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 165:59-70 (1998) - doi:10.3354/meps165059 Temperature dependence of microbial degradation organic matter in marine sediments: polysaccharide hydrolysis, oxygen consumption, and sulfate reduction C. Arnosti1,*, B. Jørgensen2, J. Sagemann2, Thamdrup2 1Marine Sciences, University North Carolina, Chapel Hill,...
Heterotrophic microbial communities cycle nearly half of net primary productivity in the ocean, and play a particularly important role transformations dissolved organic carbon (DOC). The specific means by which these mediate are largely unknown, since vast majority marine bacteria have not been isolated culture, most measurements DOC degradation rates focused on uptake metabolism either bulk or simple model compounds (e.g. amino acids sugars). Genomic investigations provide information about...
Summary The marine bacterium A lteromonas macleodii is a copiotrophic r ‐strategist, but little known about its potential to degrade polysaccharides. Here, we studied the degradation of alginate and other algal polysaccharides by . strain 83‐1 in comparison strains. Cell densities with as sole carbon source were comparable those glucose, exponential phase was delayed. genome found harbour an alginolytic system comprising five lyases, whose expression induced alginate. contains additional CAZ...
Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy extracellular enzymes to hydrolyze large substrates smaller sizes suitable for uptake. Since hydrolysis products produced outside of a cell may be lost diffusion, return on this investment is uncertain. Selfish change odds their favor by binding, partially hydrolyzing, transporting polysaccharides into periplasmic space without loss products. We expected selfish most common upper ocean, where phytoplankton produce...