A5070068600- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Marine and coastal ecosystems
- Marine Biology and Ecology Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Earthquake Detection and Analysis
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Pancreatitis Pathology and Treatment
- Cell Image Analysis Techniques
- Conducting polymers and applications
- Single-cell and spatial transcriptomics
- Enzyme Production and Characterization
- Pancreatic and Hepatic Oncology Research
- Experimental and Theoretical Physics Studies
University of North Carolina at Chapel Hill
2017-2025
Communities In Schools of Orange County
2017
Swansea University
2016
Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy extracellular enzymes to hydrolyze large substrates smaller sizes suitable for uptake. Since hydrolysis products produced outside of a cell may be lost diffusion, return on this investment is uncertain. Selfish change odds their favor by binding, partially hydrolyzing, transporting polysaccharides into periplasmic space without loss products. We expected selfish most common upper ocean, where phytoplankton produce...
Abstract Heterotrophic bacteria hydrolyze high molecular weight (HMW) organic matter extracellularly prior to uptake, resulting in diffusive loss of hydrolysis products. An alternative ‘selfish’ uptake mechanism that minimises this has recently been found be common the ocean. We investigated how HMW addition affects these two processing mechanisms surface and bottom waters at three stations North Atlantic Ocean. A pulse increased cell numbers, as well rate spectrum extracellular enzymatic...
Abstract Heterotrophic microorganisms are responsible for transforming and respiring a substantial fraction of the organic matter produced by phytoplankton in surface ocean. Much this is composed polysaccharides, high‐molecular weight (HMW) sugars. To initiate degradation must produce extracellular enzymes right structural specificity to hydrolyze these complex structures. date, most measurements enzyme activities made at situ temperatures, but atmospheric pressure. However, previous studies...
This feature compares the merits of different 3D invasion assays. We highlight paper-based cultures as an emerging platform that is readily accessible, modular in design, and capable quantifying tissue-like structures.
To elucidate the chemical and environmental conditions that promote invasion of cancer cells, an assay is needed in which landscape a tumor-like environment can be experimentally manipulated probed. The three-dimensional paper-based assays described here simulate poorly vascularized tissue allow cancerous cells to visualized quantified. These cultures are easy assemble multiple performed parallel. By using different materials control gradients formed across culture, chemotactic potential...
Heterotrophic bacteria initiate the degradation of high molecular weight organic matter by producing an array extracellular enzymes to hydrolyze complex into sizes that can be taken up cell. These bacterial communities differ spatially and temporally in composition, potentially also their enzymatic complements. Previous research has shown particle-associated considerably more active than surrounding bulk water, but most prior studies have been focused on upper ocean - there are few...
Abstract. Heterotrophic bacteria process nearly half of the organic matter produced by phytoplankton in surface ocean. Much this consists high molecular weight (HMW) biopolymers such as polysaccharides and proteins, which must initially be hydrolyzed to smaller sizes structurally specific extracellular enzymes. To assess relationships between substrate structure microbial community composition function, we concurrently determined carbohydrate abundance structural complexity, bacterial...
Supplemental Table 1. Volume of water filtered (L) for POM analyses. DepthsStn. 17 Stn.18 Stn.19 Stn.20 Surface 6.0 9.5 5.0 13.5 DCM 5.5 8.5 12.5 300 m -13.5 15.0 14.5 Meso 9.0 11.0 1500 -14.0 14.0 3000 --13.7 Bottom 13.0 Figure 1.Particulate organic carbon and the POC:PON ratio each station depth.Stations are separated by color.
Heterotrophic bacteria use extracellular enzymes to hydrolyze high molecular weight (HMW) organic matter low (LMW) hydrolysis products that can be taken into the cell. These represent a considerable investment of carbon, nitrogen, and energy, yet return on this is uncertain, since HMW substrate outside cell yields LMW lost diffusion up by scavengers do not produce 1 . However, an additional strategy utilization, ‘selfish’ uptake 2 , used for polysaccharide degradation, has recently been...