A5001788221- Reproductive System and Pregnancy
- Advanced biosensing and bioanalysis techniques
- Cellular Mechanics and Interactions
- Nanoparticle-Based Drug Delivery
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Force Microscopy Techniques and Applications
- Plant Molecular Biology Research
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Down syndrome and intellectual disability research
- Electrochemical sensors and biosensors
- Endometriosis Research and Treatment
- Ovarian cancer diagnosis and treatment
- Selenium in Biological Systems
- Cell Adhesion Molecules Research
- Plant tissue culture and regeneration
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Fungal and yeast genetics research
Swansea University
2016-2025
Houston Methodist
1999-2024
Australia New Zealand Gynaecological Oncology Group
2019-2023
Life Science Institute
2021
Cornell University
2019
Welsh Centre for Printing and Coating
2012
University of Wales
2004-2007
University of Maryland, College Park
2004
Foundation for Research and Technology Hellas
1999-2001
FORTH Institute of Molecular Biology and Biotechnology
2000
Abstract Background The endometrium is commonly infected with bacteria leading to severe disease of the uterus in cattle and humans. endometrial epithelium first line defence for this mucosal surface against Toll-like receptors (TLRs) are a critical component innate immune system detection pathogen associated molecular patterns (PAMPs). Antimicrobial peptides, acute phase proteins Mucin-1 (MUC-1) also provide non-specific defences microbes on surfaces. present study examined expression...
The conserved bifactorial endosperm box found in the promoter of wheat storage protein genes comprises two different cis elements that are thought to be involved regulating endosperm-specific gene expression. Endosperm nuclear extracts contain binding activities. One is called ESBF-I, which binds motif (EM), and other ESBF-II, GCN4-like motif(GLM). Here, we present a functional analysis low-molecular-weight glutenin on 1D1 chromosome hexaploid (LMWG-1D1) transgenic tobacco plants. Our...
Transcription repression plays important roles in preventing crucial regulatory proteins from being expressed inappropriate temporal or spatial domains. LEUNIG ( LUG ) and SEUSS SEU normally act to prevent ectopic expression of the floral homeotic gene AGAMOUS flowers. encodes a protein with sequence similarities yeast Tup1 corepressor. plant-specific similarity conserved dimerization domain LIM-domain binding 1/Chip mouse Drosophila . Despite molecular isolation , biochemical function these...
Transcription corepressors are general regulators controlling the expression of genes involved in multiple signaling pathways and developmental programs. Repression is mediated through mechanisms including stabilization a repressive chromatin structure over control regions regulation Mediator function inhibiting RNA polymerase II activity. Using whole-genome arrays we show that Arabidopsis thaliana corepressor LEUNIG, member GroTLE transcription family, regulates targets vivo. LEUNIG has...
Following oral administration, the bioavailability of progestogens is very low and highly variable, in part due to metabolism by cytochrome P450 enzymes found mucosa small intestine. Conversely, colon contains much lower levels enzymes, thus, colonic delivery may be beneficial. Microbiota are known metabolize a great number drugs, therefore, it important understand stability these hormones presence flora before developing formulations. The aim this study was investigate three progestogens:...
The Srb/Mediator, a multisubunit subcomplex of the RNA polymerase II (RNA pol II) holoenzyme has been proposed to function as control panel regulating transcription in response gene-specific activator proteins. In this report, we identify Mediator subunit Hrs1/Med3 physical target for Cyc8-Tup1, yeast transcriptional corepressor. Two-hybrid and glutathione S-transferase interaction assays show that Hrs1 can associate directly with Cyc8-Tup1. Moreover, affinity chromatography experiments,...
Endometrium of fertile women expresses progesterone-regulated Mucin 1 (MUC1) that carries selectin ligands recognized by the human blastocyst. Altered MUC1 expression at time implantation may contribute to endometrial infertility.The aim was assess in endometrium from polycystic ovary syndrome (PCOS), endometriosis, and comparison with other hormone-regulated proteins [hydroxysteroid dehydrogenase (HSD) 1, HSD2, estrogen receptor (ER) progesterone (PR)].Endometrial samples were obtained 33...
Human chorionic gonadotropin (hCG) is a key diagnostic marker of pregnancy and an important biomarker for cancers in the prostate, ovaries bladder therefore great importance diagnosis. For this purpose, new immunosensor screen-printed electrodes (SPEs) presented here. The device was fabricated by introducing polyaniline (PANI) conductive layer, via situ electropolymerization aniline, onto graphene support. PANI-coated acts as working electrode three terminal electrochemical sensor....
// Micaela Morgado 1 , Margie N. Sutton 2, 3 Mary Simmons 2 Curtis R. Warren 4 Zhen Lu Pamela E. Constantinou Jinsong Liu 5 Lewis LW. Francis 7 Steven Conlan Robert C. Bast Jr Daniel D. Carson 1, 6 Department of BioSciences, Wiess School Natural Sciences, Rice University, Houston, TX 77251, USA Experimental Therapeutics, Division Cancer Medicine, The University Texas MD Anderson Center, 77030, Graduate Biomedical Health Science Center at Stem Cell and Regenerative Biology, Harvard Cambridge,...
Exosomes are physiologically secreted nanoparticles recently established as natural delivery systems involved in cell-to-cell communication and content exchange. Due to their inherent targeting potential, exosomes currently being harnessed for the development of anti-cancer therapeutics. Clinical trials evaluating effectiveness demonstrating safety promising outcomes. However, challenging large-scale production, isolation, modification purification current limitations use naturally occurring...
During decidualization, human mesenchymal-like endometrial stromal cells undergo well characterized cellular and molecular transformations in preparation for accepting a developing embryo. Modulation of biophysical properties during decidualization is likely to be important receptivity support the embryo uterus. Here we assess including topography, roughness, adhesiveness stiffness undergoing vitro decidualization. A significant reduction cell surface roughness was observed following These...
Epithelial ovarian cancer (EOC) is an aggressive and lethal gynaecologic malignancy due to late diagnosis acquired resistance chemotherapeutic drugs, such as cisplatin. EOC metastasis commonly occurs through the extensive dissemination of multicellular aggregates, formed cells originally shed from primary tumour, within peritoneal cavity. However, little known about how cisplatin (CR) alters biophysical properties aggregates this impacts metastasis. In interdisciplinary study, light atomic...
L-selectin ligands, localized to the luminal epithelium at time of implantation, may support early stages blastocyst attachment. We have assessed expression two defined by MECA-79 and HECA-452 monoclonal antibodies, sulfotransferase GlcNAc6ST-2, involved in generation ligand epitopes, secretory phase endometrium from fertile infertile patients. Endometrial samples were obtained 33 fertile, 26 PCOS, 25 endometriosis patients diagnosed with unexplained infertility. ligands GlcNAc6ST-2 was...
Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their sitespecific biomechanical demands.Maturation involves gross morphological changes occur through a of synchronised growth and resorption generally ends at sexual maturity.The inability induce in biomaterial constructs designed for repair has been cited as major cause failure producing persistent cell-based lesions.The combination factors FGF2 TGFb1 induces accelerated articular vitro such...
Significance Mediator is a megadalton multisubunit molecular switchboard involved in gene regulation eukaryotes and structurally conserved between species. It bridges the general transcription machinery function-specific DNA binding proteins. plays dynamic role regulating wide range of processes, involving, for example, thyroid vitamin D receptors. The appears to be fine tuning activation repression expression many organisms, yet underlying mechanisms how its own function regulated remains...
Polycystic ovary syndrome (PCOS) is a common gynaecological disorder, with prevalence of up to 12% women reproductive age, and in part characterised by elevated circulating androgens aberrant expression androgen receptor (AR) the endometrium. A high percentage PCOS patients suffer from infertility, condition that appears be linked mistimed incomplete decidualisation critically affecting events surrounding embryo implantation. The aim this study was examine involvement MAGEA11, genome-wide...