A5029599635- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Wheat and Barley Genetics and Pathology
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Polysaccharides and Plant Cell Walls
- Plant Genetic and Mutation Studies
- Potato Plant Research
- Legume Nitrogen Fixing Symbiosis
- Plant Taxonomy and Phylogenetics
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Plant Pathogens and Resistance
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Genetically Modified Organisms Research
John Innes Centre
2015-2024
Norwich Research Park
2012-2024
Salk Institute for Biological Studies
2020
Chinese Academy of Sciences
2015
The University of Western Australia
2014
Institute of Genetics and Developmental Biology
2013
The Ohio State University
2011
Oregon State University
2010-2011
Agricultural Research Service
2011
United States Department of Agriculture
2011
Journal Article Binary Agrobacterium vectors for plant transformation Get access Michael Bevan Plant Breeding InstituteMaris Lane, Trumpington, Cambridge CB2 2LQ, UK Search other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 12, Issue 22, 26 November 1984, Pages 8711–8721, https://doi.org/10.1093/nar/12.22.8711 Published: 1984 history Received: 24 July Revision received: 25 October Accepted:
Bread wheat (Triticum aestivum) is a globally important crop, accounting for 20 per cent of the calories consumed by humans. Major efforts are underway worldwide to increase production extending genetic diversity and analysing key traits, genomic resources can accelerate progress. But so far very large size polyploid complexity bread genome have been substantial barriers analysis. Here we report sequencing its large, 17-gigabase-pair, hexaploid using 454 pyrosequencing, comparison this with...
Abstract Advances in genomics have expedited the improvement of several agriculturally important crops but similar efforts wheat ( Triticum spp.) been more challenging. This is largely owing to size and complexity genome 1 , lack genome-assembly data for multiple lines 2,3 . Here we generated ten chromosome pseudomolecule five scaffold assemblies hexaploid explore genomic diversity among from global breeding programs. Comparative analysis revealed extensive structural rearrangements,...
Summary Transcription factors containing a conserved DNA‐binding domain similar to that of the proto‐oncogene c‐myb have been identified in nearly all eukaryotes. MYB‐related proteins from plants generally contain two related helix‐turn‐helix motifs, R2 and R3 repeats. It was estimated Arabidopsis thaliana contains more than 100 R2R3‐MYB genes. The few cases where functional data are available suggest an important role these genes regulation secondary metabolism, control cell shape, disease...
A combination of advanced sequencing and mapping techniques is used to produce a reference genome Aegilops tauschii, progenitor the wheat D genome, providing valuable resource for comparative genetic studies. Sequencing genomes crops plants provides useful resources crop improvement breeding. Jan Dvořák, Katrien Devos, Steven Salzberg colleagues report diploid hexaploid wheat. They use ordered-clone sequencing, whole-genome shotgun BioNano optical assemble this large highly repetitive...
Summary The cell wall determines the shape of plant cells and is also primary interface for pathogen interactions. structure can be modified in response to developmental environmental cues, example strengthen create barriers ingress. ectopic lignin 1‐1 1‐2 ( eli1‐1 eli1‐2 ) mutations lead an aberrant deposition lignin, a complex phenylpropanoid polymer. We show that eli1 mutants occur cellulose synthase gene CESA3 Arabidopsis thaliana cause reduced synthesis, providing further evidence...
Although the size of an organism is a defining feature, little known about mechanisms that set final organs and whole organisms. Here we describe Arabidopsis DA1 , encoding predicted ubiquitin receptor, which sets seed organ by restricting period cell proliferation. The mutant protein encoded da1-1 allele has negative activity toward DA1-related (DAR) protein, overexpression cDNA dramatically increases wild-type plants, identifying this small gene family as important regulators in plants.
High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome in Arabidopsis thaliana. These exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180–base pair repeats. Unexpectedly, within centromeres not merely structural but also encoded several expressed genes. The flanking were densely populated by repetitive elements yet experienced normal levels recombination. genetically...
Summary Plants both produce and utilize carbohydrates have developed mechanisms to regulate their sugar status co‐ordinate carbohydrate partitioning. High levels result in a feedback inhibition of photosynthesis an induction storage processes. We used genetic approach isolate components the signalling pathway regulating starch biosynthesis. The regulatory sequences inducible ADP‐glucose pyrophosphorylase subunit ApL3 were fused negative selection marker. Of four impaired sucrose ( isi )...
Advances in genome sequencing and assembly technologies are generating many high-quality sequences, but assemblies of large, repeat-rich polyploid genomes, such as that bread wheat, remain fragmented incomplete. We have generated a new wheat whole-genome shotgun sequence using combination optimized data types an algorithm designed to deal with large complex genomes. The represents >78% the scaffold N50 88.8 kb has high fidelity input data. Our annotation combines strand-specific Illumina...
Journal Article Strcuture and transcription of the nopaline synthase gene region T-DNA Get access Michael Bevan, Bevan * Present address: Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 21Q, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Wayne M. Barnes, Barnes § 1Department Biological Chemistry, Washington University School MedicinSaint Louis, MO 63110, USA To whom reprint requests should be sent Marry-Dell Chilton Nucleic Acids Research,...
Seed size in higher plants is determined by the coordinated growth of embryo, endosperm, and maternal tissue. Several factors that act maternally to regulate seed have been identified, such as AUXIN RESPONSE FACTOR2, APETALA2, KLUH, DA1, but genetic molecular mechanisms these control are almost totally unknown. We previously demonstrated ubiquitin receptor DA1 acts synergistically with E3 ligase ENHANCER1 OF (EOD1)/BIG BROTHER final seeds Arabidopsis thaliana. Here, we describe another...
Over the past several years, Brachypodium distachyon (Brachypodium) has emerged as a tractable model system to study biological questions relevant grasses. To place its relevance in larger context of plant biology, we outline here expanding adoption grass and compare this early history another model, Arabidopsis thaliana. In context, followed an accelerated path which development genomic resources, most notably whole genome sequence, occurred concurrently with generation other experimental...
Summary Food security is a global concern and substantial yield increases in cereal crops are required to feed the growing world population. Wheat one of three most important for human livestock feed. However, complexity genome coupled with decline genetic diversity within modern elite cultivars has hindered application marker‐assisted selection (MAS) breeding programmes. A crucial step successful MAS programmes development cheap easy use molecular markers, such as single‐nucleotide...
Summary A member of the R2R3‐MYB family transcription factors was cloned from a cDNA library constructed RNA isolated differentiating pine xylem. This MYB, Pinus taeda MYB4 ( Pt MYB4), is expressed in cells undergoing lignification, as revealed by situ RT‐PCR. Electrophoretic mobility shift assays (EMSAs) showed that recombinant protein able to bind DNA motifs known AC elements. elements are ubiquitous promoters genes encoding lignin biosynthetic enzymes. Transcriptional activation using...
We have undertaken a systematic reverse genetic approach to understand R2R3-MYB gene function in Arabidopsis. Here, we report the functional characterization of MYB61 based on phenotype three independent insertion alleles. Wide-ranging screens indicated that mutants were deficient seed mucilage extrusion upon imbibition. This was expressed sporophytic tissues seed. Deposition and principal component mucilage, relatively unbranched rhamnogalacturonan, reduced mutant coats. Additional defects...