A5011605894- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- RNA Research and Splicing
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant Gene Expression Analysis
- DNA Repair Mechanisms
- Plant Genetic and Mutation Studies
- Fungal and yeast genetics research
- Genetic Mapping and Diversity in Plants and Animals
- RNA and protein synthesis mechanisms
- Genetic Syndromes and Imprinting
- Plant responses to water stress
- Light effects on plants
- Cancer-related molecular mechanisms research
Cold Spring Harbor Laboratory
2016-2025
Howard Hughes Medical Institute
2016-2025
Gordon and Betty Moore Foundation
2012-2020
Stony Brook University
2014-2017
A.S. Watson (Netherlands)
2015
Institut de Biologie de l'École Normale Supérieure
2014
Bethesda University
2005-2008
Pasadena City College
2005-2008
Science Oxford
2005-2008
University of San Francisco
2008
Eukaryotic heterochromatin is characterized by a high density of repeats and transposons, as well modified histones, influences both gene expression chromosome segregation. In the fission yeast Schizosaccharomyces pombe , we deleted argonaute, dicer, RNA-dependent RNA polymerase homologs, which encode part machinery responsible for interference (RNAi). Deletion results in aberrant accumulation complementary transcripts from centromeric heterochromatic repeats. This accompanied...
Three DNA hypomethylation mutants of the flowering plant Arabidopsis thaliana were isolated by screening mutagenized populations for plants containing centromeric repetitive arrays susceptible to digestion a restriction endonuclease that was sensitive methylated cytosines. The mutations are recessive, and at least two alleles single locus, designated DDM1 (for decrease in methylation). Amounts 5-methylcytosine reduced over 70 percent ddm1 mutants. Despite this reduction methylation levels,...
The transition from vegetative to reproductive phases during Arabidopsis development is the result of a complex interaction environmental and endogenous factors. One key regulators this LEAFY (LFY), whose threshold levels activity are proposed mediate initiation flowers. closely related APETALA1 (AP1) CAULIFLOWER (CAL) meristem identity genes also important for flower initiation, in part because their roles upregulating LFY expression. We have found that mutations FRUITFULL (FUL) MADS-box...
Fruit morphogenesis is a process unique to flowering plants, and yet little known about its developmental control. Following fertilization, fruits typically undergo dramatic enlargement that accompanied by differentiation of numerous distinct cell types. We have identified mutation in Arabidopsis called fruitfull (ful-1), which abolishes elongation the silique after fertilization. The ful-1 caused insertion DsE transposable enhancer trap element into 5' untranslated leader AGL8 MADS-box...
The genome sequence of the African oil palm, main source production, is used to predict at least 34,802 genes, including biosynthesis genes; comparison with draft South American palm reveals that two species may have diverged in New World and segmental duplications chromosome arms define palaeotetraploid origin trees. Two papers published this issue Nature deal genetics variants one most important crops use today — Elaeis guineensis its cousin oleifera. Palm accounts for almost half edible...
TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1 (TFL2/LHP1) is the only Arabidopsis protein with overall sequence similarity to (HP1) family of metazoans and S. pombe. TFL2/LHP1 represses transcription numerous genes, including flowering-time genes FLOWERING LOCUS T (FT) C (FLC), as well floral organ identity AGAMOUS (AG) APETALA 3 (AP3). These are also regulated by proteins Polycomb repressive complex 2 (PRC2), it has been proposed that represents a potential stabilizing factor PRC2...
Cytosine methylation of repetitive sequences is widespread in plant genomes, occurring both symmetric (CpG and CpNpG) as well asymmetric sequence contexts. We used the methylation-dependent restriction enzyme McrBC to profile methylated DNA using tiling microarrays Arabidopsis Chromosome 4 two distinct ecotypes, Columbia Landsberg erecta. also comparative genome hybridization copy number polymorphisms. Repeated transposable elements (TEs), especially long terminal repeat retrotransposons,...
The Arabidopsis gene DDM1 is required to maintain DNA methylation levels and responsible for transposon transgene silencing. However, rather than encoding a methyltransferase, has similarity the SWI/SNF family of adenosine triphosphate-dependent chromatin remodeling genes, suggesting an indirect role in methylation. Here we show that also histone H3 patterns. In wild-type heterochromatin, transposons silent genes are associated with methylated at lysine 9, whereas known preferentially 4....
High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome in Arabidopsis thaliana. These exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180–base pair repeats. Unexpectedly, within centromeres not merely structural but also encoded several expressed genes. The flanking were densely populated by repetitive elements yet experienced normal levels recombination. genetically...