A5020713009- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Cancer-related gene regulation
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Genetic Syndromes and Imprinting
- Antifungal resistance and susceptibility
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Cancer-related molecular mechanisms research
- Pneumocystis jirovecii pneumonia detection and treatment
- Mycorrhizal Fungi and Plant Interactions
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Chromatin Remodeling and Cancer
National Cancer Institute
2016-2025
National Institutes of Health
2016-2025
UNSW Sydney
2024
Center for Information Technology
2015
University of California, Los Angeles
2015
Center for Cancer Research
2008-2014
QB3
2008
University of California, San Francisco
2008
University of California, San Diego
2008
Howard Hughes Medical Institute
2008
Eukaryotic heterochromatin is characterized by a high density of repeats and transposons, as well modified histones, influences both gene expression chromosome segregation. In the fission yeast Schizosaccharomyces pombe , we deleted argonaute, dicer, RNA-dependent RNA polymerase homologs, which encode part machinery responsible for interference (RNAi). Deletion results in aberrant accumulation complementary transcripts from centromeric heterochromatic repeats. This accompanied...
The assembly of higher order chromatin structures has been linked to the covalent modifications histone tails. We provide in vivo evidence that lysine 9 H3 (H3 Lys ) is preferentially methylated by Clr4 protein at heterochromatin-associated regions fission yeast. Both conserved chromo- and SET domains are required for methylation vivo. Localization Swi6, a homolog Drosophila HP1, heterochomatic dependent on methylation. Moreover, an H3-specific deacetylase Clr3 β-propeller domain Rik1 Swi6...
RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe purification of an RNAi effector complex termed RITS (RNA-induced initiation gene silencing) required for heterochromatin assembly in fission yeast. The contains Ago1 (the yeast Argonaute homolog), Chp1 (a heterochromatin-associated chromodomain protein), Tas3 novel protein). In addition, small RNAs require Dicer ribonuclease their production....
The higher-order assembly of chromatin imposes structural organization on the genetic information eukaryotes and is thought to be largely determined by posttranslational modification histone tails. Here, we study a 20-kilobase silent domain at mating-type region fission yeast as model for heterochromatin formation. We find that, although H3 methylated lysine 9 (H3 Lys ) directly recruits protein Swi6/HP1, critical determinant methylation spread in cis inherited through mitosis meiosis Swi6...
Eukaryotic genomes are organized into discrete structural and functional chromatin domains. Here, we show that distinct site-specific histone H3 methylation patterns define euchromatic heterochromatic chromosomal domains within a 47-kilobase region of the mating-type locus in fission yeast. methylated at lysine 9 (H3 Lys ), its interacting Swi6 protein, strictly localized to 20-kilobase silent interval. In contrast, 4 ) is specific surrounding regions. Two inverted repeats flanking interval...
An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction focused on various aspects chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component RNA interference (RNAi) machinery (rsh1) linked RNAi pathway to several other biological processes. Comparison...
At the silent mating-type interval of fission yeast, RNA interference (RNAi) machinery cooperates with cenH, a DNA element homologous to centromeric repeats, initiate heterochromatin formation. However, in RNAi mutants, assembly can still occur at low efficiency. Here, we report that Atf1 and Pcr1, two ATF/CREB family proteins, act parallel mechanism pathway for nucleation. Deletion atf1 or pcr1 alone has little effect on silencing region, but when combined double mutants fail nucleate...
The regulation of higher-order chromosome structure is central to cell division and sexual reproduction. Heterochromatin assembly at the centromeres facilitates both kinetochore formation sister chromatid cohesion, specialized chromatin structures telomeres serves maintain length telomeric repeats, suppress recombination, aid in a bouquet-like that homologous pairing during meiosis. In fission yeast, genes encoding Argonaute, Dicer, RNA-dependent RNA polymerase factors involved interference...
In fission yeast, factors involved in the RNA interference (RNAi) pathway including Argonaute, Dicer, and RNA-dependent polymerase are required for heterochromatin assembly at centromeric repeats silent mating-type region. Previously, we have shown that RNA-induced initiation of transcriptional gene silencing (RITS) complex containing Argonaute protein small interfering RNAs (siRNAs) localizes to heterochromatic loci collaborates with via a self-enforcing RNAi loop mechanism couple siRNA...