A5090270463- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Genetic Syndromes and Imprinting
- Genetics and Neurodevelopmental Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- Plant Molecular Biology Research
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Phagocytosis and Immune Regulation
- Cancer Mechanisms and Therapy
- Gender Studies in Language
- Gender Roles and Identity Studies
- Plant Reproductive Biology
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Legume Nitrogen Fixing Symbiosis
- Genomic variations and chromosomal abnormalities
- Cancer-related gene regulation
- Immune Cell Function and Interaction
Université Paris Sciences et Lettres
2016-2024
Inserm
2011-2024
Institut Curie
2011-2024
Centre National de la Recherche Scientifique
2011-2024
University of Lisbon
2019-2024
Instituto Gulbenkian de Ciência
2024
Sorbonne Université
2016
University of Oxford
2012-2015
Génétique et biologie du développement
2013-2014
Laboratoire de Biologie du Développement
2013
The Arabidopsis gene DDM1 is required to maintain DNA methylation levels and responsible for transposon transgene silencing. However, rather than encoding a methyltransferase, has similarity the SWI/SNF family of adenosine triphosphate-dependent chromatin remodeling genes, suggesting an indirect role in methylation. Here we show that also histone H3 patterns. In wild-type heterochromatin, transposons silent genes are associated with methylated at lysine 9, whereas known preferentially 4....
X chromosome inactivation involves multiple levels of chromatin modification, established progressively and in a stepwise manner during early development. The chromosomal protein Smchd1 was recently shown to play an important role DNA methylation CpG islands (CGIs), late step the pathway that is required for long-term maintenance gene silencing. Here we show inactive (Xi) CGI can occur via either Smchd1-dependent or -independent pathways. methylation, primary pathway, acquired gradually over...
The Smchd1 gene encodes a large protein with homology to the SMC family of proteins involved in chromosome condensation and cohesion. Previous studies have found that has an important role CpG island (CGI) methylation on inactive X (Xi) stable silencing some Xi genes. In this study, using genome-wide expression analysis, we showed is required for around 10% genes Xi, apparently independent CGI hypomethylation, and, moreover, these nonrandomly occur clusters. Additionally, at cluster four...
Histone acetyl transferases (HATs) play distinct roles in many cellular processes and are frequently misregulated cancers. Here, we study the regulatory potential of MYST1-(MOF)-containing MSL NSL complexes mouse embryonic stem cells (ESCs) neuronal progenitors. We find that both influence transcription by targeting promoters TSS-distal enhancers. In contrast to flies, complex is not exclusively enriched on X chromosome, yet it crucial for mammalian chromosome regulation as specifically...
The third X-inactivation meeting 'Fifty years of research', which celebrated the fiftieth anniversary Mary Lyon's formulation hypothesis, was an EMBO workshop held in Oxford, UK, July 2011. This conference brought together usual suspects from field, as well younger researchers, to discuss recent advances research. Here, we review results presented at and highlight some exciting progress that has been made. We also future challenges for aim further our understanding developmental regulation X...
The chromosomal protein SMCHD1 plays an important role in epigenetic silencing at diverse loci, including the inactive X chromosome, imprinted genes, and facioscapulohumeral muscular dystrophy locus. Although homology with canonical SMC family proteins suggests a chromosome organization, mechanisms underlying function target site selection remain poorly understood. Here we show that forms active GHKL-ATPase homodimer, contrasting complexes, which exist as tripartite ring structures. Electron...
In the Arabidopsis accession Columbia, 5S rDNA is located in pericentromeric heterochromatin of chromosomes 3, 4, and 5. Both a major some minor rRNA species are expressed from 4 5, whereas genes on chromosome 3 not transcribed. Here, we show that methylation reduced 2-day-old seedlings versus 4-day-old or older aerial plant tissues, most abundantly at this stage. Similarly, when demethylated by 5-azacytidine treatment via decrease DNA methylation1 (ddm1) mutation, expression increased. We...
Mammalian X-chromosome inactivation (XCI) enables dosage compensation between XX females and XY males. It is an essential process its absence in individuals results early lethality due primarily to extra-embryonic defects. This sensitivity X-linked gene tissues difficult reconcile with the reported tendency of escape from XCI these tissues. The precise transcriptional status inactive X chromosome different lineages has mainly been examined using transgenes or vitro differentiated stem cells...
Transposable elements (TEs) are mobile genetic that constitute on average 45% of mammalian genomes. Their presence and activity in genomes represent a major source variability. While this is an important driver genome evolution, TEs can also have deleterious effects their hosts. A growing number studies focused the role brain, both physiological pathological contexts. In believed to be for neuronal plasticity. neurological age-related disorders, aberrant may contribute disease etiology,...
Reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSCs) is a major leap towards personalised approaches to disease modelling and cell-replacement therapies. However, we still lack the ability fully control epigenetic status iPSCs, which hurdle for their downstream applications. Epigenetic fidelity can be tracked by genomic imprinting, phenomenon dependent on DNA methylation, frequently perturbed in iPSCs yet unknown reasons. To try understand causes underlying these...
Abstract Most autosomal genes are thought to be expressed from both alleles, with some notable exceptions, including imprinted and showing random monoallelic expression (RME). The extent nature of RME has been the subject debate. Here we investigate several candidate in F1 hybrid mouse cells before after differentiation, define how they become persistently, monoallelically expressed. Clonal is not present embryonic stem cells, but observe high frequencies monoallelism neuronal progenitor by...
Abstract Long interspersed element-1 (LINE-1 or L1) retrotransposons constitute the largest transposable element (TE) family in mammalian genomes and contribute prominently to inter- intra-individual genetic variation. Although most L1 elements are inactive, some evolutionary younger remain intact genetically competent for transcription occasionally retrotransposition. Despite being generally more abundant gene-poor regions, full-length L1s (FL-L1) also enriched around specific classes of...
LINE-1 (L1) elements represent the largest family of transposable in mammalian genomes, making up approximately 21% and 17% mouse human genome, respectively. While most these are no longer intact, a significant number with potentially functional promoters, primarily from evolutionarily youngest families both species, scattered throughout genome. These L1 autonomously expressed encode RNAs, which however not necessarily translated into proteins, nor do complete their retrotransposition cycle...