A5025560685- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Plant nutrient uptake and metabolism
- Gut microbiota and health
- Probiotics and Fermented Foods
- Legume Nitrogen Fixing Symbiosis
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Plant Genetic and Mutation Studies
- Clostridium difficile and Clostridium perfringens research
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Digestive system and related health
- Plant Reproductive Biology
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Colorectal Cancer Screening and Detection
- Adipose Tissue and Metabolism
- Ecology and Conservation Studies
- Diet and metabolism studies
- Plant tissue culture and regeneration
Centre National de la Recherche Scientifique
2011-2024
Inserm
2011-2024
Université Clermont Auvergne
2006-2024
Genetique Reproduction and Developpement
2003-2024
Lallemand (Canada)
2012-2024
Health Solutions (Sweden)
2024
Lallemand (United Kingdom)
2012-2024
Gregor Mendel Institute of Molecular Plant Biology
2021
Clermont Université
2008-2019
University of Geneva
2005-2010
Background. Recurrent Clostridium difficile infection (RCDI) is associated with repeated antibiotic treatment and the enhanced growth of antibiotic-resistant microbes. This study tested hypothesis that patients RCDI would harbor large numbers microbes fecal microbiota transplantation (FMT) reduce number genes. Methods. In a single center study, (n = 20) received FMT from universal donors via colonoscopy. Stool samples were collected 3) prior to following FMT. DNA was extracted shotgun...
Constitutive heterochromatin comprising the centromeric and telomeric parts of chromosomes includes DNA marked by high levels methylation associated with histones modified repressive marks. These epigenetic modifications silence transcription ensure stable inheritance this inert state. Although environmental cues can alter marks lead to modulation genes located in euchromatic chromosomes, there is no evidence that external stimuli globally destabilize silencing constitutive heterochromatin....
Significance Similar to changes in DNA sequence, induced or naturally occurring variation cytosine methylation can impact gene expression. How distinct states of genes and transposons, called epialleles, emerge is not well understood. Here, we report that combining identical genomes with drastically different patterns the same individual results an epigenomic shock characterized by widespread Many novel epialleles found parents are formed at whereas transposons often experience decreased...
Clinical effects of antimicrobials and probiotics in combination have been reported, however, little is known about their impact on gut microbiota its resistome. In this study 16S rRNA gene amplicon, shotgun metagenomics sequencing antibiotic resistance (ABR) microarray were used fecal samples 70 healthy participants, taken at four time points probiotic (Lactobacillus rhamnosus R0011 Lactobacillus helveticus R0052) placebo groups to profile the bacterial resistome following administration...
Abstract In flowering plants, heterochromatin is demarcated by the histone variant H2A.W, elevated levels of linker H1, and specific epigenetic modifications, such as high DNA methylation at both CG non-CG sites. How H2A.W regulates organization interacts with other heterochromatic features unclear. Here, we create a h2a.w null mutant via CRISPR-Cas9, h2a.w-2 , to analyze in vivo function H2A.W. We find that antagonizes deposition H1 accessibility are moderately decreased heterochromatin....
In the Arabidopsis accession Columbia, 5S rDNA is located in pericentromeric heterochromatin of chromosomes 3, 4, and 5. Both a major some minor rRNA species are expressed from 4 5, whereas genes on chromosome 3 not transcribed. Here, we show that methylation reduced 2-day-old seedlings versus 4-day-old or older aerial plant tissues, most abundantly at this stage. Similarly, when demethylated by 5-azacytidine treatment via decrease DNA methylation1 (ddm1) mutation, expression increased. We...
A physical map of a pericentromeric region chromosome 5 containing 5S rDNA locus and spanning ∼1000 kb was established using the CIC YAC clones. Three arrays were resolved in this contig by PFGE analysis we have mapped different types sequences between these three blocks. units from each 5, chromosomes 3 4, isolated PCR. total 38 new DNA obtained. Two repeated exist: major variant with 0.5-kb repeats one short (251 bp) only detected on 11A3 3. Although displayed noticeable heterogeneity,...
ABSTRACT A commercially available product containing three probiotic bacterial strains ( Lactobacillus helveticus R0052, Bifidobacterium longum subsp. infantis R0033, and bifidum R0071) was previously shown in animal trials to modulate both T H 1 2 immune responses. Clinical studies on this combination of bacteria have also positive health effects against seasonal winter diseases rotavirus infection. The goal study use a well-established vitro intestinal epithelial (HT-29) cell model that...
Abstract Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 MAIN define an alternative pathway independent Mutants for or exhibit release appear impaired condensation pericentromeric heterochromatin. Phylogenetic analysis suggests not only encode a...
Genome-wide transcriptional analysis in intestinal epithelial cells (IEC) can aid elucidating the impact of single versus multi-strain probiotic combinations on immunological and cellular mechanisms action. In this study we used human expression microarray chips an vitro cell model to investigate three bacteria, Lactobacillus helveticus R0052 (Lh-R0052), Bifidobacterium longum subsp. infantis R0033 (Bl-R0033) bifidum R0071 (Bb-R0071) individually combination, a surface-layer protein (SLP)...
Introduction Few minimally invasive options for sampling the small intestinal (SI) luminal fluid exist to study SI microbiota in health and disease. To address lack of tools methods GI regions that are difficult access, Nimble Science developed a fully autonomous passive method, Small Intestine MicroBiome Aspiration (SIMBA™) capsule, convenient, high-quality, reliable diet-microbiota interactions SI. Methods The sealing efficacy microbial DNA preservation capacity SIMBA capsules was first...
Thus far, no transcription factor IIIA (TFIIIA) from higher plants has been cloned and characterized. We have characterized TFIIIA ribosomal protein L5 Arabidopsis thaliana. Primary sequence comparison revealed a high divergence of AtTFIIIA relatively conservation AtL5 when compared with other organisms. The cDNA encodes nine Cys2‐His2‐type zinc fingers, 23 amino acid spacer between fingers 1 2, 66 4 5, 50 non‐finger C‐terminal tail. Aside the acids required for proper finger folding, is...
Summary In this study, polymorphisms in genes encoding porcine adiponectin (ADIPOQ) and its receptors (ADIPOR1 ADIPOR2) were evaluated for associations with reproductive traits a Landrace sow population. Sixteen SNPs identified, among these, found between five SNPs. Heterozygous multiparous females SNP ADIPOQ EF601160 :c.178G>A had fewer stillborn piglets ( P < 0.05) shorter weaning‐to‐oestrus intervals 0.05). Multiparous bearing the mutant allele :c.*1094_1095insC gave birth to...
Arabidopsis MOM1 is required for the heritable maintenance of transcriptional gene silencing (TGS). Unlike many other factors, depletion evokes transcription at selected loci without major changes in DNA methylation or histone modification. These retain unusual, bivalent chromatin properties, intermediate to both euchromatin and heterochromatin. The structure previously suggested an integral nuclear membrane protein with chromatin-remodeling actin-binding activities. Unexpected results...
In order to understand the appropriate use of potentially probiotic Gram-positive microbes through their introduction in gut microbiome, it is necessary influence individual bacteria on host-response system at a cellular level. present study, we have shown that lipopolysaccharides, flagellated Gram-negative bacteria, and yeast interact differently with human intestinal epithelial cells custom-designed expression microarray evaluating 17 specific pathways. Only lipopolysaccharides induced...