A5003066782- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- RNA modifications and cancer
- Nuclear Structure and Function
- Plant Molecular Biology Research
- RNA Research and Splicing
- Ecology and Conservation Studies
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Animal Genetics and Reproduction
- Plant Ecology and Soil Science
- Wnt/β-catenin signaling in development and cancer
- Connective tissue disorders research
- Ubiquitin and proteasome pathways
- Seedling growth and survival studies
- Folate and B Vitamins Research
- Cell Image Analysis Techniques
- Heme Oxygenase-1 and Carbon Monoxide
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
Clermont Université
2017-2023
Inserm
2008-2023
Université Clermont Auvergne
1999-2023
Centre National de la Recherche Scientifique
1996-2023
Genetique Reproduction and Developpement
1991-2002
Institut Pasteur
1991
A viable autosomal recessive mutation (named fch, or ferrochelatase deficiency) causing jaundice and anemia in mice arose a mutagenesis experiment using ethylnitrosourea. Homozygotes (fch/fch) display hemolytic anemia, photosensitivity, cholestasis, severe hepatic dysfunction. Protoporphyrin is found at high concentration erythrocytes, serum, liver. Ferrochelatase activity various tissues 2.7-6.3% of normal. Heterozygotes (+/fch) are not anemic have normal liver function; they sensitive to...
SUN-domain proteins belong to a gene family including classical Cter-SUN and mid-SUN subfamilies differentiated by the position of SUN domain within protein. Although present in animal plant species, have so far remained poorly described. Here, we used combination genetics, yeast two-hybrid planta transient expression methods better characterize Arabidopsis thaliana. First, validated protein subfamily as monophyletic group conserved from plant. (AtSUN1 AtSUN2) (AtSUN3 AtSUN4) expressed...
ABSTRACT The linker of nucleoskeleton and cytoskeleton (LINC) complex is an evolutionarily well-conserved protein bridge connecting the cytoplasmic nuclear compartments across membrane. While recent data support its function in morphology meiosis, involvement chromatin organisation has not been studied plants. Here, 3D imaging methods have used to investigate interphase nuclei model plant Arabidopsis thaliana which heterochromatin clusters conspicuous domains called chromocentres....
A physical map of a pericentromeric region chromosome 5 containing 5S rDNA locus and spanning ∼1000 kb was established using the CIC YAC clones. Three arrays were resolved in this contig by PFGE analysis we have mapped different types sequences between these three blocks. units from each 5, chromosomes 3 4, isolated PCR. total 38 new DNA obtained. Two repeated exist: major variant with 0.5-kb repeats one short (251 bp) only detected on 11A3 3. Although displayed noticeable heterogeneity,...
NucleusJ 1.0, an ImageJ plugin, is a useful tool to analyze nuclear morphology and chromatin organization in plant animal cells. 2.0 new release of NucleusJ, which image processing achieved more quickly using command-lineuser interface. Starting with large collection 3D nuclei, segmentation can be performed by the previously developed Otsu-modified method or gift-wrapping method, taking better account indentations unstained nucleoli. These two complementary methods are compared for their...
The Arabidopsis thaliana genome comprises around 1,000 copies of 5S rRNA genes encoding both major and minor rRNAs. In mature wild-type leaves, the are silent. Using different mutants DNA methyltransferases ( met1 , cmt3 ), components RNAi pathway ago4 ) or post-translational histone modifier hda6/sil1 we show that corresponding proteins needed to maintain proper methylation patterns at heterochromatic rDNA repeats. reverse transcription–PCR cytological analyses, report a decrease CG CNG...
The nucleoskeleton forms a filamentous meshwork under the nuclear envelope and contributes to regulation of shape gene expression. To understand how Arabidopsis (Arabidopsis thaliana) physically connects periphery in plants, we investigated protein KAKU4 sought for functional regions responsible its localization at periphery. We identified 3 conserved peptide motifs within N-terminal region KAKU4, which are required intermolecular interactions with itself, interaction CROWDED NUCLEI (CRWN),...
Summary In vivo , we have already shown that only two of the 5S rDNA array blocks Arabidopsis thaliana genome produce mature rRNAs. Deletions and point mutations were introduced in an rDNA‐transcribed region its 5′‐ 3′‐flanks order to analyse their effects on transcription activity. vitro revealed different control regions. One essential for initiation was identified 5′‐flanking sequence. The major sequence determinants a TATA‐like motif (−28 −23), GC dinucleotide (−12 −11), 3‐bp AT‐rich (−4...
The plant-specific RNA polymerases Pol IV and V are essential to RNA-directed DNA methylation (RdDM), which also requires activities from RDR2 (RNA-Dependent Polymerase 2), DCL3 (Dicer-Like 3), AGO4 (Argonaute), DRM2 (Domains Rearranged Methyltransferase 2). RdDM is dedicated the of target sequences include transposable elements, regulatory regions several protein-coding genes, 5S rRNA-encoding (rDNA) arrays. In this paper, we have studied expression 5S-210 transcript, a marker silencing...
Ribosome biogenesis is critical for eukaryotic cells and requires coordinated synthesis of the protein rRNA moieties ribosome, which are therefore highly regulated. 5S ribosomal RNA, an essential component large subunit, transcribed by RNA polymerase III specifically transcription factor IIIA (TFIIIA). To obtain insight into regulation transcription, we have investigated expression exon-skipped (ES) exon-including (EI) TFIIIA transcripts, two transcript isoforms that result from alternative...
A strategy based on random PCR amplification was used to isolate new repetitive elements of Arabidopsis thaliana . One the product analyzed by this approach contained a tandem minisatellite sequence composed 33 bp repeated units. The genomic locus corresponding isolated screening lambda library. New related loci were also from library with 14 mer oligonucleotide representing region conserved among different Alignment consensus for each allowed definition an core that shows strong...
Summary 5S ribosomal DNA (5S rDNA) is organized in tandem repeats on chromosomes 3, 4 and 5 Arabidopsis thaliana . One part of the rDNA located within heterochromatic chromocenters, other fraction forms loops with euchromatic features that emanate from chromocenters. We investigated whether A. heterochromatin, particularly rDNA, modified when changing culture conditions (cultivation growth chamber versus greenhouse). Nuclei challenged tissues displayed larger total, as well fractions,...
ABSTRACT The nucleoskeleton forms a filamentous meshwork under the nuclear envelope and contributes to regulation of morphology gene expression. To understand how Arabidopsis physically connects periphery, we investigated protein KAKU4 sought for functional regions responsible its localization at periphery. Computational predictions identified three evolutionary conserved peptide motifs within N-terminal region KAKU4. Functional analysis revealed that these are required homomerization KAKU4,...
In order to characterize new GC-rich minisatellites present in the Arabidopsis thaliana genome, a genomic library was screened at low stringency with probe containing nine repeated-units of minisatellite (CMs1) previously identified. Both and minisatellite-like elements were The minisatellites, tandemly-repeated structure, all contain thaliana-core sequence defined (Tourmente et al., 1994). sequences occur genome copy are weakly polymorphic between ecotypes. genetic mapping these markers has...
Mid-SUN proteins are a neglected family of conserved type III membrane ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them functions at the nuclear envelope endoplasmic reticulum (ER). In this study, high-resolution confocal light microscopy is used to explore localisation SUN3 SUN4 perinuclear region, topology, study role mid-SUNs on morphology. The ER reinforced by identification protein interaction between membrane-bound...