A5048989958- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Microbial metabolism and enzyme function
- Cellular Mechanics and Interactions
- Biotin and Related Studies
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Nuclear Structure and Function
- Microbial bioremediation and biosurfactants
- Opinion Dynamics and Social Influence
- Galectins and Cancer Biology
- Polysaccharides and Plant Cell Walls
- Plant responses to water stress
- Neural Networks and Applications
- Transgenic Plants and Applications
- RNA Research and Splicing
- Photoreceptor and optogenetics research
Oxford Brookes University
2018-2024
University of Warwick
2018
The endoplasmic reticulum (ER) is a highly dynamic polygonal membrane network composed of interconnected tubules and sheets (cisternae) that forms the first compartment in secretory pathway involved protein translocation, folding, glycosylation, quality control, lipid synthesis, calcium signalling, metabolon formation. Despite its central role this plethora biosynthetic, metabolic physiological processes, there little quantitative information on ER structure, morphology or dynamics. Here we...
Summary The plant endoplasmic reticulum ( ER ) is crucial to the maintenance of cellular homeostasis. consists a dynamic and continuously remodelling network tubules cisternae. Several conserved membrane proteins have been implicated in formation plants, such as RHD 3 reticulon proteins. Despite recent work mammalian yeast cells, detailed molecular mechanisms organization plants remain largely unknown. Recently, novel network‐shaping called Lunapark LNP identified cells. Here we identify two...
Abstract The actin cytoskeleton is the driver of gross ER remodelling and movement positioning other membrane‐bound organelles such as Golgi bodies. Rapid membrane a feature most plant cells important for normal cellular processes, including targeted secretion, immunity signalling. Modifications to through pharmacological agents Latrunculin B phalloidin, or disruption myosin function also affect structure and/or dynamics. Here, we investigate impact changes in on dynamics well return...
The availability of quantification methods for subcellular organelle dynamic analysis has increased rapidly over the last 20 years. application these techniques to contiguous structures that exhibit remodelling a range scales and orientations is challenging, as 'movement' rarely corresponds traditional, qualitative classifications types movement. plant endoplasmic reticulum represents particular challenge it itself an entirely in constant state flux gross remodelling, controlled by...
In plants, the endoplasmic reticulum (ER) and Golgi bodies are not only in close proximity, but also physically linked. This unique organization raises questions about nature of transport vectors carrying cargo between two organelles. Same as metazoan yeast cells, it was suggested that is transported from ER to cisternae via COPII-coated vesicles produced at ribosome-free exit sites (ERES). Recent developments mammalian cell research suggest, though, COPII helps select secretory cargo, does...
Determining protein-protein interactions is vital for gaining knowledge on cellular and metabolic processes including enzyme complexes metabolons. Förster resonance energy transfer with fluorescence lifetime imaging microscopy (FRET-FLIM) an advanced methodology that allows the quantitative detection of interactions. In this method, proteins interest interaction studies are fused to different fluorophores such as enhanced green fluorescent protein (eGFP; donor molecule) monomeric red (mRFP;...
Methane is a potent greenhouse gas, which has contributed to approximately fifth of global warming since pre-industrial times. The agricultural sector produces significant methane emissions, especially from livestock, waste management and rice cultivation. Rice fields alone generate around 9% total anthropogenic emissions. produced in waterlogged paddy by methanogenic archaea, transported the atmosphere through aerenchyma tissue plants. Thus, bioengineering with catalysts detoxify en route...
Integration of multiomics and imaging identifies a glycosyltransferase enzyme regulating endoplasmic reticulum sizes.
The plant Golgi apparatus is responsible for the processing of proteins received from endoplasmic reticulum (ER) and their distribution to multiple destinations within cell. matrix components, such as golgins, have been identified suggested function putative tethering factors mediate physical connections between bodies ER network. Golgins are anchored membrane by C-terminus either through transmembrane domains or interaction with small regulatory GTPases. golgin N-terminus contains long...
The Anaphase Promoting Complex (APC/C), a large cullin-RING E3-type ubiquitin ligase, constitutes the ultimate target of Spindle Assembly Checkpoint (SAC), an intricate regulatory circuit that ensures high fidelity chromosome segregation in eukaryotic organisms by delaying onset anaphase until each is properly bi-oriented on mitotic spindle. Cell-division cycle protein 20 homologue (CDC20) key regulator APC/C function mitosis. formation APC/CCDC20 complex required for ubiquitination and...
The endoplasmic reticulum (ER) links to multiple organelles through membrane contact sites (MCS), which play critical roles in signal transduction, cell homeostasis and stress response. However, studying the behaviour functions of MCS plants is still challenging, partially due lack site-specific markers. Here, we used an optogenetic reporter, LiMETER (Light-inducible Membrane-Tethered cortical ER), study structure dynamics ER-PM (EPCS) plants. Upon blue light activation, recruited EPCS...
Summary The plant endoplasmic reticulum (ER) is crucial to the maintenance of cellular homeostasis. ER consists a dynamic and continuously remodelling network tubules cisternae. Several conserved membrane proteins have been implicated in formation plants, such as RHD3 reticulon family proteins. Despite recent work mammalian yeast cells, detailed molecular mechanisms organisation plants still remain largely unknown. Recently novel network-shaping called Lunapark identified cells. Here we...
In plants, the cortical ER network is connected to plasma membrane through ER-PM contact sites (EPCS), whose structures are maintained by EPCS resident proteins and cytoskeleton. Strong co-alignment between cytoskeleton observed in but little known of how regulated at EPCS. Here we have used a yeast-two-hybrid screen subsequent vivo interaction studies plants FRET-FLIM analysis, identify two microtubule binding proteins, KLCR1 (Kinesin Light Chain Related protein 1) IQD2 (IQ67-Domain 2) that...
Mid-SUN proteins are a neglected family of conserved type III membrane ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them functions at the nuclear envelope endoplasmic reticulum (ER). In this study, high-resolution confocal light microscopy is used to explore localisation SUN3 SUN4 perinuclear region, topology, study role mid-SUNs on morphology. The ER reinforced by identification protein interaction between membrane-bound...
SUMMARY The endoplasmic reticulum (ER) is a central eukaryotic organelle with tubular network made of hairpin proteins linked by hydrolysis GTP nucleotides. Among post-translational modifications initiated at the ER level, glycosylation most common reaction. However, our understanding impact on structure remains unclear. Here, we show that Exostosin-1 (EXT1) glycosyltransferase, an enzyme involved in N -glycosylation, key regulator morphology and dynamics. We have integrated multi-omics data...