Adiponectin, the most abundantly synthesized protein in adipose tissue, has plieotropic effects on liver, muscle, endothelium, placenta, and other tissues. We examined direct of recombinant porcine adiponectin ovarian granulosa cells vitro. demonstrate that adiponectin, at physiologically relevant levels (10–25 μg/ml), provokes expression genes associated with periovulatory remodeling follicle over a time frame 6–24 h. These include cyclooxygenase-2, prostaglandin E synthase, vascular...

The present experiment was undertaken to determine the effects of dietary supplements folic acid and vitamin B12 given from 3 wk before 8 after calving on lactational performance metabolism 24 multiparous Holstein cows assigned 6 blocks 4 each according their previous milk production. Supplementary at 0 or 2.6 g/d 0.5 were used in a 2 x factorial arrangement. increased production 38.0 +/- 0.9 41.4 1.0 kg/d crude protein yield 1.17 0.02 1.25 0.03 kg/d. It also plasma Gly, Ser, Thr, total...

Adiponectin is an adipocyte-derived hormone that plays important role in lipid metabolism and glucose homeostasis. Objectives of this study were 1) to determine the presence distribution adiponectin its receptors 1 2 (adipoR1 adipoR2) porcine tissues; 2) characterize pig adiponectin, adipoR1, adipoR2 mRNA levels various fat depots from three different breeds pigs; 3) study, stromal-vascular cell culture, effects leptin tumor necrosis factor-alpha (TNFalpha) on gene expression. To end,...

The direct effects of recombinant porcine leptin on granulosa cells were studied to test the hypothesis that leptin, acting through nuclear transcription factor signal transducer and activator 3 (STAT-3), modulates sterol regulatory element-binding protein 1 (SREBP1) thereby increasing steroidogenesis. In in culture over 48 h, at 10 ng/ml increased progesterone accumulation 3-fold while it was reduced by 1000 ng/ml. Leptin had no effect progression cell cycle nor frequency death. treatment...

Abstract Carnosine, as a meat constituent, was previously shown to exhibit some pro‐oxidative effects during in vitro digestion of burger meal model the presence high (supplemental) level ascorbic acid (AA). In current study, dietary carnosine were assessed combo model, including intrinsic (low, LCar) and enriched (intermediate, MCar; high, HCar) ground pork, with or without levels AA and/or fructose simulated beverages. Increased (MCar showed antioxidant activity, irrespective phase AA. At...

The porcine leptin receptor complementary DNA was cloned and sequenced the gene expression evaluated in ovary. An open reading frame of 3498 nt cDNA amplified from pig liver mRNA by RT-PCR. Sequence homology with extracellular, transmembrane, cytoplasmic domains human, mouse, rat, sheep, cow receptors varied between 45% 90%. Leptin present kidney, liver, spleen, lung, brain, testis, uterus, ovary, corpus luteum (CL), theca, granulosa cells. abundance transcripts protein during luteinization...

Four polymorphisms in the swine leptin (LEP) gene were characterized and evaluated for association with economically important production traits Yorkshire, Landrace Duroc pigs. Our results show that these are generally of low frequency or absent pig populations. Two (A2845T T3469C) may be associated (P < 0.0078) feed intake growth rate

Abstract Embryonic diapause is a poorly understood phenomenon of reversible arrest embryo development prior to implantation. In many carnivores, such as the mink (Neovison vison), obligate characterizes each gestation. Embryo reactivation controlled by uterus mechanisms that remain elusive. Because polyamines are essential regulators cell proliferation and growth, it was hypothesized they trigger reactivation. To test this, mated females were treated with α-difluoromethylornithine, an...

The objectives of the study were to investigate effects dietary supplementation flax hulls and/or oil on activity antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX)) in plasma mammary gland relative mRNA abundance genes dairy cows. A total eight cows used a replicated 4 × Latin square design. There four treatments: control with no (CONT), 9·88 % DM (HULL), 500 g oil/d infused abomasum (COFO), (HUFO). Plasma GPX tended decrease supplementation....

The effects of flax meal (FM) on the activity antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT)) in blood, mammary tissue ruminal fluid, oxidative stress indicators (thiobarbituric acid-reactive substances(TBARS) 1,1-diphenyl-2-picrylhydrazyl-scavenging activity) milk, plasma fluid dairy cows were determined.The mRNA abundance stress-related genes was assessed tissue. A total eight Holstein used a double 4 x Latin square design. There four...

The main objective of the present study was to identify novel oocyte-specific genes in three different species: bovine, mouse, and Xenopus laevis. To achieve this goal, two powerful technologies were combined: a polymerase chain reaction (PCR)-based cDNA subtraction, microarrays. Three subtractive libraries consisting 3456 clones established enriched for transcripts. Sequencing analysis positive insert-containing resulted following classification: 53% corresponded known cDNAs, 26% classified...

The obese (ob) gene isolated from ob/ob mice in 1994 is expressed specifically adipose tissue. product, leptin, regulates food intake, energy expenditure and puberty. Reported herein a 2477 bp mRNA sequence for the pig gene, which includes complete coding sequence. Two different ob cDNAs were identified, differed by inclusion or exclusion of single glutamine codon at position + 49 protein encodes 4.5 kb transcript, specific to Southern blot analysis fat lean pigs showed DNA polymorphism...

The impacts of a teat being suckled or not in first parity on its development, gene expression, and milk yield the next were studied. Forty-seven first-parity sows (Sus scrofa) divided into 2 groups: i) same teats subsequent lactations (controls, CTL; n = 22); ii) different (treated, TRT; 25). In lactation, over half (Teats 1, 2, 5, 6, 7 from 1 side udder, Teats 3, 4, other side) sealed with tape so that they nonfunctional. During CTL group had as whereas opposite for TRT group. both...