A5074604343- Lepidoptera: Biology and Taxonomy
- Plant and animal studies
- Botany, Ecology, and Taxonomy Studies
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Wheat and Barley Genetics and Pathology
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Malaria Research and Control
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Insect-Plant Interactions and Control
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Parasites and Host Interactions
- Mitochondrial Function and Pathology
- Plant-Microbe Interactions and Immunity
- Light effects on plants
- Botanical Research and Chemistry
- Plant Genetic and Mutation Studies
- Insect behavior and control techniques
Yunnan University
2017-2025
Max Planck Institute for Developmental Biology
2010-2023
Jiangsu Institute of Parasitic Diseases
2021-2023
Nanjing Medical University
2021-2023
Yibin University
2022-2023
Chengdu Institute of Biology
2017-2020
Chinese Academy of Sciences
2017-2020
Max Planck Institute for Biology
2015
Guangdong Medical College
2014
Johns Hopkins University
2011-2013
We present whole-genome assemblies of four divergent Arabidopsis thaliana strains that complement the 125-Mb reference genome sequence released a decade ago. Using newly developed reference-guided approach, we assembled large contigs from 9 to 42 Gb Illumina short-read data Landsberg erecta (L er -1), C24, Bur-0, and Kro-0 strains, which have been sequenced as part 1,001 Genomes Project for this species. alignments against sequence, first reduced complexity de novo assembly later integrated...
Abstract Background The mitochondrial genome of higher plants is unusually dynamic, with recombination and nonhomologous end-joining (NHEJ) activities producing variability in size organization. Plant DNA also generally displays much lower nucleotide substitution rates than mammalian or yeast systems. Arabidopsis these features expedites characterization the surveillance gene MSH1 (MutS 1 homolog), lending itself to detailed study de novo activity. In present study, we investigated...
Plants, like animals, use several lines of defense against pathogen attack. Prominent among genes that confer disease resistance are those encoding nucleotide-binding site-leucine-rich repeat (NB-LRR) proteins. Likely due to selection pressures caused by pathogens, NB-LRR the most variable gene family in plants, but there appear be species-specific limits number a genome. Allelic diversity within an individual is also increased obligatory outcrossing, which leads genome-wide heterozygosity....
The previously recognised closely related species Graphium (Pazala) mandarinus (Oberthür, 1879) and G. (P.) sichuanica (Koiwaya, 1993) are shown to comprise seven as a result of both molecular morphological analysis. Molecular dating analysis is also performed on the group in order investigate divergence time taxa. Two taxa, garhwalica (Katayama, 1988) stat. nov. paphus (de Nicéville, 1886) nov., raised from subspecific specific status; hoeneanus Cotton Hu nom. rev. separated at level; two...
Rapid detection of allelic variation and identification advantage haplotypes responsible for spike related traits play a crucial role in wheat yield improvement. The released genome sequence hexaploid (Chinese Spring) provides an extraordinary opportunity rapid natural promotes breeding application. Here, selection signals genome-wide association study (GWAS) were conducted traits. Based on the genotyping results by 90K SNP chip, 192 common samples from southwest China analyzed. One hundred...
Abstract Light initiates chloroplast biogenesis by activating photosynthesis-associated genes encoded not only the nuclear but also plastidial genome, how photoreceptors control gene expression remains enigmatic. Here we show that photoactivation of phytochromes triggers plastid-encoded ( PhAPG s) stimulating assembly bacterial-type RNA polymerase (PEP) into a 1000-kDa complex. Using forward genetic approaches, identified REGULATOR OF CHLOROPLAST BIOGENESIS (RCB) as dual-targeted...
Gametocytes are essential for Plasmodium transmission, but little is known about the mechanisms that lead to their formation. Using piggyBac transposon-mediated insertional mutagenesis, we screened parasites no longer form mature gametocytes, which led isolation of 29 clones (insertional gametocyte-deficient mutants) fail gametocytes. Additional analysis revealed 16 genes putatively responsible loss gametocytogenesis, none has been previously implicated in gametocytogenesis. Transcriptional...
Abstract Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded ( PhAPG s). s are transcribed a bacterial-type RNA polymerase (PEP), but how phytochromes in nucleus activate gene expression remains enigmatic. We report here forward genetic screen Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as necessary component of phytochrome signaling for activation. NCP is...
We have developed a quantum dot-based microRNA nanosensor for point mutation assays using primer generation-mediated rolling circle amplification. The proposed method exhibits high sensitivity with detection limit of as low 50.9 aM and large dynamic range 7 orders magnitude from 0.1 fM to 1 nM. Importantly, this can be further applied analyze the mir-196a2 in lung tissues non small-cell cancer patients.
Second-site mutagenesis was performed on the argonaute1-33 (ago1-33) hypomorphic mutant, which exhibits reduced sense transgene posttranscriptional gene silencing (S-PTGS). Mutations in FIERY1, a positive regulator of cytoplasmic 5'-to-3' EXORIBONUCLEASE4 (XRN4), and SUPERKILLER3 (SKI3), member SKI complex that threads RNAs directly to 3'-to-5' exoribonuclease exosome, compensated AGO1 partial deficiency restored S-PTGS with 100% efficiency. Moreover, xrn4 ski3 single mutations provoked...
RNA quality control (RQC) eliminates aberrant RNAs based on their atypical structure, whereas posttranscriptional gene silencing (PTGS) both and functional through the sequence-specific action of short interfering (siRNAs). The Arabidopsis thaliana mutant smd1b was identified in a genetic screen for PTGS deficiency, revealing involvement SmD1, component Smith (Sm) complex, PTGS. smd1a single mutants are viable, but double is embryo-lethal, indicating that SmD1 function essential. SmD1b...
Wheat is one of the major food crops in world. However, stripe rust fungus significantly decreases wheat yield and quality. In present study, transcriptomic metabolite analyses were conducted R88 (resistant line) CY12 (susceptible cultivar) during Pst-CYR34 infection due to limited availability information regarding underlying mechanisms governing wheat-pathogen interactions. The results revealed that Pst promoted genes metabolites involved phenylpropanoid biosynthesis. key enzyme gene TaPAL...
The malaria parasite Plasmodium falciparum infects humans and first targets the liver where liver-stage parasites undergo pre-erythrocytic replication. Liver-stage antigen-1 (LSA-1) is currently only identified P. protein for which expression restricted to stages. Yet, importance of LSA-1 development remains unknown. Here we deleted in NF54 strain analysed lsa-1(-) throughout their life cycle. sporozoites had normal gliding motility invasion into hepatocytes. Six days after infection a...
Abstract Motivation: The sequencing of over a thousand natural strains the model plant Arabidopsis thaliana is producing unparalleled information at genetic level for researchers. To enable rapid exploitation these data functional proteomics studies, we have created resource visualization protein and proteomic datasets sequenced A. thaliana. Results: 1001 Proteomes portal can be used to visualize amino acid substitutions or non-synonymous single-nucleotide polymorphisms in individual...
Abstract Besides regulating splicing, the conserved spliceosome component SmD1 (Small nuclear ribonucleoprotein D1)b promotes posttranscriptional silencing of sense transgenes (S-PTGS [post-transcriptional genesilencing]). Here, we show that PRP39 (Pre-mRNA-processing factor 39)a also plays a role in S-PTGS Arabidopsis thaliana. However, PRP39a and SmD1b actions appear distinct both splicing S-PTGS. Indeed, RNAseq-based analysis expression level alternative prp39a smd1b mutants identified...