A5042195612- Plant Molecular Biology Research
- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Genomics and Chromatin Dynamics
- Plant Stress Responses and Tolerance
- Nematode management and characterization studies
- Plant Gene Expression Analysis
- Agronomic Practices and Intercropping Systems
- Cancer-related molecular mechanisms research
- Plant responses to water stress
- Plant Reproductive Biology
- Circular RNAs in diseases
- Photosynthetic Processes and Mechanisms
- Islanding Detection in Power Systems
- Insect symbiosis and bacterial influences
- MicroRNA in disease regulation
- Smart Cities and Technologies
- Computational Physics and Python Applications
- CRISPR and Genetic Engineering
Institut des Sciences des Plantes de Paris Saclay
2017-2024
Université Paris Cité
2012-2024
Centre National de la Recherche Scientifique
2012-2024
Université Paris-Saclay
2017-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Sorbonne Paris Cité
2013-2023
Université Paris-Sud
2017-2023
Institut des Sciences du Végétal
2012-2018
University of California, Riverside
2013-2017
Plant (United States)
2015
Translational regulation contributes to plasticity in metabolism and growth that enables plants survive a dynamic environment. Here, we used the precise mapping of ribosome footprints (RFs) on mRNAs investigate translational under control sublethal hypoxia stress conditions seedlings Arabidopsis thaliana. Ribosomes were obtained by differential centrifugation or immunopurification digested with RNase I generate footprint fragments deep-sequenced. Comparison RF number position genic regions...
Eukaryotic transcriptomes contain a major non-protein-coding component that includes precursors of small RNAs as well long noncoding RNA (lncRNAs). Here, we utilized the mapping ribosome footprints on to explore translational regulation coding and in roots Arabidopsis thaliana shifted from replete deficient phosphorous (Pi) nutrition. Homodirectional changes steady-state mRNA abundance translation were observed for all but 265 annotated protein-coding genes. Of translationally regulated...
Significance Flooding due to extreme weather events can be highly detrimental plant development and yield. Speedy recovery following stress removal is an important determinant of tolerance, yet mechanisms regulating this remain largely uncharacterized. We identified a regulatory network in Arabidopsis thaliana that controls water loss senescence influence from prolonged submergence. Targeted control the molecular facilitating here could potentially improve performance crops flood-prone areas.
Abstract The complex and dynamic three-dimensional organization of chromatin within the nucleus makes understanding control gene expression challenging, but also opens up possible ways to epigenetically modulate expression. Because plants are sessile, they evolved sophisticated rapidly in response environmental stress, that thought be coordinated by changes conformation mediate specific cellular physiological responses. However, what extent how stress induces reorganization remains poorly...
The root system is crucial for acquisition of resources from the soil. In legumes, efficiency mineral and water uptake by roots may be reinforced due to establishment symbiotic relationships with mycorrhizal fungi interactions soil rhizobia. Here, we investigated role miR396 in regulating architecture model legume Medicago truncatula. Analyses promoter-GUS fusions suggested that mtr-miR396a miR396b genes are highly expressed tips, preferentially transition zone, display distinct expression...
Abiotic and biotic stresses limit crop productivity. Exposure to a non-lethal stress, referred as priming, can allow plants survive subsequent otherwise lethal conditions; the priming effect persists even after prolonged stress-free period. However, molecular mechanisms underlying are not fully understood. Here, we investigated basis of heat-shock memory role in Arabidopsis thaliana. Comprehensive analysis transcriptome-wide changes gene expression alternative splicing primed non-primed...
Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based platform in plants evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance splicing inhibitors. mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of Studies structural basis inhibitor binding SGRs corroborate phenotype. This can be used interrogate molecular functions key biomolecules engineer...
Posttranscriptional regulation of mRNA mediated by methylation at the N6 position adenine (N6-methyladenosine [m6A]) has profound effects on transcriptome in plants. Focused studies across eukaryotes offer glimpses into processes governed m6A throughout developmental and disease states. However, we lack an understanding dynamics regulatory potential during biotic stress Here, provide a comprehensive look both short-term long-term responses to pathogen signaling Arabidopsis (Arabidopsis...
Auxin action is mediated by a complex signalling pathway involving transcription factors of the auxin response factor (ARF) family. In Arabidopsis, microRNA160 (miR160) negatively regulates three ARF genes (ARF10/ARF16/ARF17) and therefore controls several developmental processes, including primary lateral root growth. Here, we analysed role miR160 in development nodulation Medicago truncatula Gaertn. Bioinformatic analyses identified two main mtr-miR160 variants (mtr-miR160abde mtr-miR160c)...
Alternative splicing (AS) of pre-mRNAs in plants is an important mechanism gene regulation environmental stress tolerance but plant signals involved are essentially unknown. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) mediated by mitogen-activated protein kinases and the majority PTI defense genes regulated MPK3, MPK4 MPK6. These responses have been mainly analyzed at transcriptional level, however many factors direct targets MAPKs. Here, we studied alternative...
Nuclear speckle RNA binding proteins (NSRs) act as regulators of alternative splicing (AS) and auxin-regulated developmental processes such lateral root formation in Arabidopsis thaliana. These were shown to interact with specific alternatively spliced mRNA targets at least one structured lncRNA, named Alternative Splicing Competitor RNA. Here, we used genome-wide analysis RNAseq monitor the NSR global role on multiple tiers gene expression, including processing AS. NSRs affect AS 100s genes...
The root-knot nematode Meloidogyne incognita secretes specific effectors (MiEFF) and induces the redifferentiation of plant root cells into enlarged multinucleate feeding 'giant cells' essential for development. Immunolocalizations revealed presence MiEFF18 protein in salivary glands M. juveniles. In planta, localizes to nuclei giant demonstrating its secretion during plant-nematode interactions. A yeast two-hybrid approach identified nuclear ribonucleoprotein SmD1 as a partner tomato...
Abiotic stresses profoundly affect plant growth and development limit crop productivity. Pre-mRNA splicing is a major form of gene regulation that helps plants cope with various stresses. Serine/arginine (SR)-rich factors play key role in pre-mRNA to regulate different biological processes under stress conditions. Alternative (AS) SR transcripts other stress-responsive genes generates multiple splice isoforms contribute protein diversity, modulate expression, tolerance. Here, we investigated...
Isolated nuclei provide access to early steps in gene regulation involving chromatin as well transcript production and processing. Here, we describe transfer of the isolation from tagged specific cell types (INTACT) monocot rice (Oryza sativa L.). The purification biotinylated was redesigned by replacing outer nuclear-envelope-targeting domain nuclear tagging fusion (NTF) protein with an nuclear-envelope-anchored domain. This modified NTF combined codon-optimized Escherichia coli BirA a...
Abstract Root-knot nematodes (RKNs) are among the most damaging pests of agricultural crops. Meloidogyne is an extremely polyphagous genus that can infect thousands plant species. A few genes for resistance (R-genes) to RKN suitable use in crop breeding have been identified, but virulent strains and species emerged render these R-genes ineffective. Secretion effectors targeting functions mediates reprogramming root cells into specialized feeding cells, giant essential development...
In plants, the detection of microbe-associated molecular patterns (MAMPs) induces primary innate immunity by activation mitogen-activated protein kinases (MAPKs). We show here that MAMP-activated MAPK MPK6 not only modulates defense through transcriptional regulation but also via ribosomal translation machinery. To understand effects on ribosomes and their constituent proteins (RPs), polysomes, monosomes phosphorylation status RPs, MAMP-treated WT mpk6 mutant plants were analysed....
Small non coding RNAs (smRNA) participate in the regulation of development, cell differentiation, adaptation to environmental constraints and defense responses plants. They negatively regulate gene expression by degrading specific mRNA targets, repressing their translation or modifying chromatin conformation through homologous interaction with target loci. MicroRNAs (miRNA) short-interfering (siRNA) are generated from long double stranded RNA (dsRNA) that cleaved into 20- 24-nucleotide...
Abstract The SF3B complex, a multiprotein component of the U2 snRNP spliceosome, plays crucial role in recognizing branch point sequence and facilitates spliceosome assembly activation. Several chemicals that bind SF3B1 PHF5A subunits complex inhibit splicing. We recently generated splicing inhibitor-resistant mutant named S F3B1 G EX1A R ESISTANT 4 ( SGR4 ) using CRISPR-mediated directed evolution, whereas O verexpression-PHF5A esistance , OGR was by expressing an engineered version...