A5048138760- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Advanced Cellulose Research Studies
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Biofuel production and bioconversion
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Polysaccharides Composition and Applications
- Biochemical and Structural Characterization
- Yeasts and Rust Fungi Studies
- Plant biochemistry and biosynthesis
- DNA Repair Mechanisms
- Biotin and Related Studies
- Plant Micronutrient Interactions and Effects
- Growth and nutrition in plants
- Wheat and Barley Genetics and Pathology
- Microtubule and mitosis dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- RNA Research and Splicing
Deakin University
2024-2025
National Bioproducts Institute (South Africa)
2025
La Trobe University
2021-2024
Agriculture and Food
2024
The University of Melbourne
2018-2024
Nanyang Technological University
2023
AgriBio
2023
University of Lausanne
2016-2020
Institut des Sciences du Végétal
2010-2013
Centre National de la Recherche Scientifique
2011-2013
The root system is crucial for acquisition of resources from the soil. In legumes, efficiency mineral and water uptake by roots may be reinforced due to establishment symbiotic relationships with mycorrhizal fungi interactions soil rhizobia. Here, we investigated role miR396 in regulating architecture model legume Medicago truncatula. Analyses promoter-GUS fusions suggested that mtr-miR396a miR396b genes are highly expressed tips, preferentially transition zone, display distinct expression...
Abstract Microtubules are filamentous structures necessary for cell division, motility and morphology, with dynamics critically regulated by microtubule-associated proteins (MAPs). Here we outline the molecular mechanism which MAP, COMPANION OF CELLULOSE SYNTHASE1 (CC1), controls microtubule bundling to sustain plant growth under salt stress. CC1 contains an intrinsically disordered N-terminus that links microtubules at evenly distributed points through four conserved hydrophobic regions. By...
Auxin action is mediated by a complex signalling pathway involving transcription factors of the auxin response factor (ARF) family. In Arabidopsis, microRNA160 (miR160) negatively regulates three ARF genes (ARF10/ARF16/ARF17) and therefore controls several developmental processes, including primary lateral root growth. Here, we analysed role miR160 in development nodulation Medicago truncatula Gaertn. Bioinformatic analyses identified two main mtr-miR160 variants (mtr-miR160abde mtr-miR160c)...
During plant growth and defense, cell cycle activity needs to be coordinated with wall integrity. Little is known about how coordination achieved. Here we investigated in Arabidopsis thaliana seedlings by studying the impact of damage (CWD, caused cellulose biosynthesis inhibition) on cytokinin homeostasis, gene expression shape root tips. CWD inhibited increased transition zone width an osmo-sensitive manner. These results were correlated CWD-induced, changes homeostasis. Expression...
Significance Cellulose is the most abundant biopolymer on Earth and has many potential industrial applications, such as renewable energy sustainable materials. Here we report apo UDP-glucose (UDP-Glc)–bound crystal structures of catalytic domain Arabidopsis thaliana CESA3. Our results offer a structural basis for how substrate UDP-Glc metal ion, Mn 2+ , which required cellulose synthesis, are coordinated in plant CESAs. Furthermore, our structure reveals that CESAs may form homodimers...
In the model plant Arabidopsis (Arabidopsis thaliana), absence of essential macro-nutrient phosphate reduces primary root growth through decreased cell division and elongation, requiring alterations to polysaccharide-rich wall surrounding cells. Despite its importance, regulation synthesis in response low levels is not well understood. this study, we show that plants increase cellulose roots under limiting conditions, which leads changes thickness structure wall. These contribute reduced...
Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence critical agricultural characteristics, including fruit ripening and organ abscission. Middle lamellae enriched in pectin polysaccharides, specifically homogalacturonan (HG). Here, we identify a plant-specific Arabidopsis DUF1068 protein, NKS1/ELMO4, that is required for integrity adhesion. NKS1 localizes to the Golgi apparatus loss of results changes structure function. The nks1...
Transcription termination has important regulatory functions, impacting mRNA stability, localization and translation potential. Failure to appropriately terminate transcription can also lead read-through the synthesis of antisense RNAs which have profound impact on gene expression. The Transcription-Export (THO/TREX) protein complex plays an role in coupling with splicing export mRNA. However, little is known about THO/TREX control termination. In this work, we show that two proteins...
ABSTRACT Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence critical agricultural characteristics, including fruit ripening and organ abscission. Middle lamellae enriched in pectin polysaccharides, specifically homogalacturonan (HG). Here, we identify a plant-specific Arabidopsis DUF1068 protein, NKS1, that is required for integrity adhesion. NKS1 localises to the Golgi apparatus loss of protein results changes structure function. The...
Abstract In the model plant Arabidopsis thaliana , absence of essential macro-nutrient phosphate reduces primary root growth through decreased cell division and elongation, requiring alterations to polysaccharide-rich wall surrounding cells. Despite its significance, regulation synthesis in response low levels is not well understood. this study, we show that plants increase cellulose roots under limiting conditions, which leads changes thickness structure wall. These contribute reduced...
Knowledge about how and where proteins interact provides a pillar for cell biology. Biotin-related proximity labelling approaches are efficiently monitoring protein interactions but may have labelling-related drawbacks. Here, we introduce pupylation-based (PUP-IT) as tool interaction detection in plant We show that PUP-IT readily confirmed extended several known complexes, across different types of systems. To further demonstrate the power PUP-IT, used system to identify complex underpin...
Isoxaben is a pre-emergent herbicide used to control broadleaf weeds. While the phytotoxic mechanism not completely understood, isoxaben interferes with cellulose synthesis. Certain mutations in synthase complex proteins can confer tolerance; however, these cause compromised synthesis and perturbed plant growth, rendering them unsuitable as tolerance traits. We conducted genetic screen identify new genes associated by screening selection of Arabidopsis thaliana T-DNA mutants. found that...
Plant cells possess robust and flexible cell walls composed primarily of cellulose, a polysaccharide that provides structural support enables expansion. Cellulose is synthesised by the Synthase A (CESA) catalytic subunits, which form cellulose synthase complexes (CSCs). While significant progress has been made in unravelling CSC function, trafficking CSCs involvement post-translational modifications synthesis remain poorly understood. In order to deepen our understanding biosynthesis, this...
Summary Isoxaben is a pre‐emergent herbicide used to control broadleaf weeds. While the phytotoxic mechanism not completely understood, isoxaben interferes with cellulose synthesis. Certain mutations in synthase complex proteins can confer tolerance; however, these cause compromised synthesis and perturbed plant growth, rendering them unsuitable as tolerance traits. We conducted genetic screen identify new genes associated by screening selection of Arabidopsis thaliana T‐DNA mutants. found...
<title>Abstract</title> Knowledge about how and where proteins interact provides a pillar for cell biology. Protein proximity-labelling has emerged as an important tool to detect protein interactions. Here, biotin-related proximity labelling approaches are by far the most commonly used, but may have labelling-related drawbacks. we use pupylation-based (PUP-IT) interaction detection in plants. We show that PUP-IT readily confirmed interactions several known complexes across different types of...