A5014158131- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Advanced Cellulose Research Studies
- Plant Gene Expression Analysis
- Cellular transport and secretion
- Biofuel production and bioconversion
- Plant Surface Properties and Treatments
- Plant-Microbe Interactions and Immunity
- Enzyme Production and Characterization
- Light effects on plants
- Plant Stress Responses and Tolerance
- Microtubule and mitosis dynamics
- Plant responses to water stress
- Lipid Membrane Structure and Behavior
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Species Distribution and Climate Change
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Surface Modification and Superhydrophobicity
- Berry genetics and cultivation research
University of Toronto
2020-2025
The University of Melbourne
2012-2024
Max Planck Institute of Molecular Plant Physiology
2014-2021
University of British Columbia
2008-2017
Ghent University
2015
Vlaams Instituut voor Biotechnologie
2015
Canada's Michael Smith Genome Sciences Centre
2014
University of Bristol
2013
McGill University
2008-2011
Developing plant embryos depend on nutrition from maternal tissues via the seed coat and endosperm, but mechanisms that supply nutrients to have remained elusive. Sucrose, major transport form of carbohydrate in plants, is delivered phloem then secreted feed embryo. Here, we show filling Arabidopsis thaliana requires three sucrose transporters SWEET11, 12, 15. 15 exhibit specific spatiotemporal expression patterns developing seeds, only a sweet11;12;15 triple mutant showed severe defects,...
Abstract ATP binding cassette (ABC) transporters play diverse roles, including lipid transport, in all kingdoms. ABCG subfamily that are encoded as half-transporters require dimerization to form a functional ABC transporter. Different dimer combinations may transport substrates have been predicted from mutant phenotypes. In Arabidopsis thaliana, analyses shown ABCG11/WBC11 and ABCG12/CER5 required for export the epidermis protective cuticle. The objective of this study was determine whether...
The actin and microtubule cytoskeletons regulate cell shape across phyla, from bacteria to metazoans. In organisms with walls, the wall acts as a primary constraint of shape, generation specific depends on cytoskeletal organization for deposition and/or expansion. higher plants, cortical microtubules help organize construction by positioning delivery cellulose synthase (CesA) complexes guiding their trajectories orient newly synthesized microfibrils. cytoskeleton is required normal...
Eukaryotic endoplasmic reticulum (ER)-plasma membrane (PM) contact sites are evolutionarily conserved microdomains that have important roles in specialized metabolic functions such as ER-PM communication, lipid homeostasis, and Ca2+ influx. Despite recent advances knowledge about site components yeast (Saccharomyces cerevisiae) mammals, relatively little is known the functional significance of these structures plants. In this report, we characterize Arabidopsis (Arabidopsis thaliana)...
The seeds of Arabidopsis thaliana and many other plants are surrounded by a pectinaceous mucilage that aids in seed hydration germination. Mucilage is synthesized during development within maternally derived coat secretory cells (MSCs), released to surround the upon imbibition. FEI1/FEI2 receptor-like kinases SOS5 extracellular GPI-anchored protein were shown previously act on pathway regulates synthesis cellulose roots. Here, we demonstrate both FEI2 also play role mucilage. Disruption or...
Abstract The secretion of cell wall polysaccharides through the trans-Golgi network (TGN) is required for plant elongation. However, components mediating post-Golgi pectin and hemicellulose, two major polysaccharides, are largely unknown. We identified evolutionarily conserved YPT/RAB GTPase Interacting Protein 4a (YIP4a) YIP4b (formerly YIP2), which form a TGN-localized complex with ECHIDNA (ECH) in Arabidopsis thaliana. localization YIP4 ECH proteins at TGN interdependent influences VHA-a1...
Abstract As the most abundant biopolymer on Earth, cellulose is a key structural component of plant cell wall. Cellulose produced at plasma membrane by synthase (CesA) complexes (CSCs), which are assembled in endomembrane system and trafficked to membrane. While several proteins that affect CesA activity have been identified, components regulate CSC assembly trafficking remain unknown. Here we show STELLO1 2 Golgi-localized can interact with CesAs control quantity. In absence STELLO...
Abstract Lipid secretion from epidermal cells to the plant surface is essential create protective cuticle. Cuticular waxes are unusual secretory products, consisting of a variety highly hydrophobic compounds including saturated very-long-chain alkanes, ketones, and alcohols. These synthesized in endoplasmic reticulum (ER) but must be trafficked plasma membrane for export by ATP-binding cassette transporters. To test hypothesis that wax components via endomembrane system packaged...
Abstract Microtubules are filamentous structures necessary for cell division, motility and morphology, with dynamics critically regulated by microtubule-associated proteins (MAPs). Here we outline the molecular mechanism which MAP, COMPANION OF CELLULOSE SYNTHASE1 (CC1), controls microtubule bundling to sustain plant growth under salt stress. CC1 contains an intrinsically disordered N-terminus that links microtubules at evenly distributed points through four conserved hydrophobic regions. By...
Abstract Differentiation of the Arabidopsis thaliana seed coat cells includes a secretory phase where large amounts pectinaceous mucilage are deposited to specific domain cell wall. During this phase, Golgi stacks had cisternae with swollen margins and trans-Golgi networks consisting interconnected vesicular clusters. The proportion producing was determined by immunogold labeling transmission electron microscopy using an antimucilage antibody, CCRC-M36. percentage found contain supports...
Abstract The cuticle is a protective layer that coats the primary aerial surfaces of land plants and mediates plant interactions with environment. It synthesized by epidermal cells composed cutin polyester matrix embedded covered cuticular waxes. Recently, we have discovered novel regulatory mechanism wax biosynthesis involves ECERIFERUM7 (CER7) ribonuclease, core subunit exosome. We hypothesized at onset production, CER7 ribonuclease degrades an mRNA specifying repressor CER3, biosynthetic...
Significance Unlike in animals, postembryonic development plants is highly flexible and allows them to modulate their growth patterns response external signals or as part of endogenous developmental programs. Differential cell elongation a widely used program respond signals. Asymmetric distribution the plant hormone indole-acetic acid (auxin) mediated by plasma membrane localized auxin carriers crucial for differential elongation. Our results identify distinct mechanisms trafficking influx...
The evolution of the plant vasculature was essential for emergence terrestrial life. Xylem vessels are solute-transporting elements in that possess secondary wall thickenings deposited intricate patterns. Evenly dispersed microtubule (MT) bands support formation these thickenings, but how MTs direct cell synthesis during this process remains largely unknown. Cellulose is major constituent and synthesized by plasma membrane-localized cellulose synthases (CesAs) whose catalytic activity...
Xanthophylls are a class of carotenoids that important micronutrients for humans. They often found esterified with fatty acids in fruits, vegetables, and certain grains, including bread wheat (Triticum aestivum). Esterification promotes the sequestration accumulation carotenoids, thereby enhancing stability, particularly tissues such as harvested grain. Here, we report on plant xanthophyll acyltransferase (XAT) is both necessary sufficient esterification XAT contains canonical...
Significance Nucleotide sugars, the activated sugar donors essential for processes such as cell wall biosynthesis and protein lipid glycosylation are predominantly made in cytosol. However, a highly diverse range of glycosyltransferases that located within Golgi lumen, mediate above-mentioned reactions. Thus, transport nucleotide sugars across membrane into lumen is crucial growth development many species including microorganisms, plants, humans. In this study, we identify functionally...
The order of enzymatic activity across Golgi cisternae is essential for complex molecule biosynthesis. However, an inability to separate has meant that the cisternal distribution most resident proteins, and their underlying localization mechanisms, are unknown. Here, we exploit differences in surface charge intact perform separation early late subcompartments. We determine protein glycan abundance profiles Golgi; over 390 proteins identified, including 136 new additions, with 180...
Significance This is a comprehensive study of Epsin-like accessory proteins in Arabidopsis , for which so far only limited data are available. We identify two members as major at the TGN, controlling vacuolar and subset secretory transport. By interaction studies with AP complexes, genetic interactions, enhanced-resolution microscopy, our work reveals existence independent, parallel transport pathways, originate from spatially separate regions TGN. Our thus provides strong molecular,...
Energy is essential for all cellular functions in a living organism. How cells coordinate their physiological processes with energy status and availability thus an important question. The turnover of actin cytoskeleton between its monomeric filamentous forms major drain eukaryotic cells. However, how dynamics are regulated by ATP levels remain largely unknown plant Here, we observed that seedlings impaired target rapamycin complex 1 (TORC1), either mutation the key component, RAPTOR1B , or...
Plant cell walls are polysaccharide-based extracellular matrices that surround all plant cells. Cell provide support and protection to the cell, while also remaining flexible enough allow growth by expansion. important renewable resources wall polysaccharides can be processed into components of food, materials, or biofuels, making modification a key goal biotechnology. However, plants sense status their via largely unknown mechanisms, collectively called “cell signaling”. Here, we review...