A5037790003- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Advanced Cellulose Research Studies
- Photosynthetic Processes and Mechanisms
- Advanced Nanomaterials in Catalysis
- Nanoplatforms for cancer theranostics
- Bioinformatics and Genomic Networks
- Plant Gene Expression Analysis
- Nanoparticle-Based Drug Delivery
- Legume Nitrogen Fixing Symbiosis
- Enzyme Production and Characterization
- Light effects on plants
- Nanocluster Synthesis and Applications
- RNA modifications and cancer
- Redox biology and oxidative stress
- Liver Disease Diagnosis and Treatment
- Metal-Catalyzed Oxygenation Mechanisms
- Cancer-related gene regulation
- Pancreatic function and diabetes
- Fungal and yeast genetics research
- Food composition and properties
University of Cambridge
2015-2024
Hubei University
2011-2024
Fujian Agriculture and Forestry University
2017-2019
Jiaxing University
2019
Leverhulme Trust
2018
Durham University
2002
As one of the most abundant polysaccharides on Earth, xylan will provide more than a third sugars for lignocellulosic biofuel production when using grass or hardwood feedstocks. Xylan is characterized by linear β(1,4)-linked backbone xylosyl residues substituted glucuronic acid, 4- O -methylglucuronic acid arabinose, depending plant species and cell types. The biological role these decorations unclear, but they have major influence properties polysaccharide. Despite recent isolation several...
With the completion of Arabidopsis genome, many hypothetical proteins have been predicted without any information on their expression, subcellular localisation and function. We performed proteomic analysis sequentially extracted from enriched cell wall fractions separated by two-dimensional gel electrophoresis (2-DE). The were identified peptide mass fingerprinting using matrix-assisted laser desorption/ionisation-time flight (MALDI-TOF) spectrometry genomic database searches. This is part a...
Mannans are hemicellulosic polysaccharides that have previously been implicated as structural constituents of cell walls and storage reserves but which may serve other functions during plant growth development. Several members the Arabidopsis cellulose synthase-like A (CSLA) family shown to synthesise mannan in vitro when heterologously expressed. It has also found CSLA7 is essential for embryogenesis, suggesting a role product To determine whether CSLA proteins responsible glucomannan...
Abstract As the most abundant biopolymer on Earth, cellulose is a key structural component of plant cell wall. Cellulose produced at plasma membrane by synthase (CesA) complexes (CSCs), which are assembled in endomembrane system and trafficked to membrane. While several proteins that affect CesA activity have been identified, components regulate CSC assembly trafficking remain unknown. Here we show STELLO1 2 Golgi-localized can interact with CesAs control quantity. In absence STELLO...
Plant cryptochromes undergo blue light-dependent phosphorylation to regulate their activity and abundance, but the protein kinases that phosphorylate plant have remained unclear. Here we show photoexcited Arabidopsis cryptochrome 2 (CRY2) is phosphorylated in vivo on as many 24 different residues, including 7 major phosphoserines. We demonstrate four closely related Photoregulatory Protein Kinases (previously referred MUT9-like kinases) interact with CRY2. Analyses of ppk123 ppk124 triple...
Arabinogalactan proteins (AGPs) are a family of plant extracellular proteoglycans involved in many physiological events. AGPs often anchored to the side plasma membrane and highly glycosylated with arabinogalactan (AG) polysaccharides, but molecular function this glycosylation remains largely unknown. The β-linked glucuronic acid (GlcA) residues AG polysaccharides have been shown vitro bind calcium pH-dependent manner. Here, we used Arabidopsis (Arabidopsis thaliana) mutants four...
Abstract Hemicellulose polysaccharides influence assembly and properties of the plant primary cell wall (PCW), perhaps by interacting with cellulose to affect deposition bundling fibrils. However, functional differences between hemicelluloses such as glucomannan, xylan, xyloglucan (XyG) remain unclear. As most abundant hemicellulose, XyG is considered important in eudicot PCWs, but plants devoid show relatively mild phenotypes. We report here that a patterned β-galactoglucomannan (β-GGM)...
Abstract The Arabidopsis thaliana protein GOLGI-LOCALIZED NUCLEOTIDE SUGAR TRANSPORTER (GONST1) has been previously identified as a GDP-d-mannose transporter. It hypothesized that GONST1 provides precursors for the synthesis of cell wall polysaccharides, such glucomannan. Here, we show in vitro can transport all four plant GDP-sugars. However, gonst1 mutants have no reduction glucomannan quantity and detectable alterations other polysaccharides. By contrast, class glycosylated sphingolipids...
Xylan is a crucial component of many plant primary and secondary cell walls. However, the structure function xylan in dicotyledon wall not well understood. Here, we characterized that specific to tissues enriched Arabidopsis Unlike previously described xylans, this carries pentose linked 1-2 α-1,2-d-glucuronic acid (GlcA) side chains on β-1,4-Xyl backbone. The frequent precisely regular spacing GlcA substitutions every six xylosyl residues along backbone also unlike observed xylan. Molecular...
Fungi are an important source of enzymes for saccharification plant polysaccharides and production biofuels. Understanding the regulation induction expression genes encoding these is still incomplete. To explore mechanism, we analysed response industrially fungus Aspergillus niger to wheat straw, with a focus on events occurring shortly after exposure substrate. RNA sequencing showed that transcriptional 6h straw was very different from at 24h same For example, less than half carbohydrate...
Plant lignocellulosic biomass can be a source of fermentable sugars for the production second generation biofuels and biochemicals. The recalcitrance this plant material is one major obstacles in its conversion into sugars. Biomass primarily composed secondary cell walls, which made cellulose, hemicelluloses lignin. Xylan, hemicellulose, binds to cellulose microfibril hypothesised form an interface between lignin cellulose. Both softwood hardwood xylan carry glucuronic acid side branches. As...
The interaction between mannan polysaccharides and cellulose microfibrils contributes to cell wall properties in some vascular plants, but the molecular arrangement of nature bonding remain unknown. Previous studies have shown that is important maintaining Arabidopsis (Arabidopsis thaliana) seed mucilage architecture, Cellulose Synthase-Like A2 (CSLA2) synthesizes a glucomannan backbone, which Mannan α-Galactosyl Transferase1 (MAGT1/GlycosylTransferase-Like6/Mucilage Related10) might...
Summary The Poaceae family of plants provides cereal crops that are critical for human and animal nutrition, also, they an important source biomass. Interacting plant cell wall components give rise to recalcitrance digestion; thus, understanding the molecular architecture is improve biomass properties. Xylan main hemicellulose in grass walls. Recently, we reported structural variation xylans, suggesting functional specialisation distinct interactions with cellulose lignin. Here, investigated...
Summary Polysaccharide structural complexity not only influences cell wall strength and extensibility but also hinders pathogenic biotechnological attempts to saccharify the wall. In certain species tissues, glucuronic acid side groups on xylan exhibit arabinopyranose or galactose decorations whose genetic evolutionary basis is completely unknown, impeding efforts understand their function engineer digestibility. Genetics polysaccharide profiling were used identify responsible loci in...
The extracellular matrix is a vital compartment in plants with prominent role defence against pathogen attack. Using maize cell suspension culture system and elicitors, responses to attack that are localised the were examined by proteomic approach. Elicitor treatment of cultures induced rapid change phosphorylation status peroxidases, apparent disappearance putative beta-N-acetylglucosamonidase, accumulation secreted xylanase inhibitor protein. Onset response was attended an...
Humans are unable to synthesize l-ascorbic acid (AsA), yet it is required as a cofactor in many critical biochemical reactions. The majority of human dietary AsA obtained from plants. In Arabidopsis thaliana, GDP-mannose pyrophosphorylase (GMPP), VITAMIN C DEFECTIVE1 (VTC1), catalyzes rate-limiting step synthesis: the formation GDP-Man. this study, we identified two nucleotide sugar pyrophosphorylase-like proteins, KONJAC1 (KJC1) and KJC2, which stimulate activity VTC1. kjc1kjc2 double...
In response to blue light, cryptochromes photoexcite and interact with signal partners transduce almost synchronously in plants. The detailed mechanism of CRY-mediated light signaling remains unclear: the photobiochemical reactions cryptochrome are transient synchronous, thus making monitoring analysis each step difficult plant cells. this study, we reconstituted Arabidopsis CRY2 pathway mammalian cells investigated biological role heterologous system, eliminating interferences other...
All members of the DUF579 family characterized so far have been described to affect integrity hemicellulosic cell wall component xylan: GXMs are glucuronoxylan methyltransferases catalyzing 4-O-methylation glucuronic acid on xylan; IRX15 and IRX15L, although their enzymatic activity is unknown, required for xylan biosynthesis and/or deposition. Here we show that members, AGM1 AGM2, a different plant component, highly glycosylated arabinogalactan proteins (AGPs).
Nanozymes that mimic the catalytic reaction of natural enzymes show great prospects for tumor therapy because they possess properties nanomaterials and enzymes. However, therapeutic effect nanozymes is restricted by low efficiency specific oxidation–reduction systems in microenvironment (TME), such as overexpressed glutathione (GSH) level. In order to improve efficiency, a multifunctional nanozyme, Cu/zeolitic imidazolate framework-67 (ZIF-67)/Prussian blue (PB), was designed through...