A5065577207- Polysaccharides and Plant Cell Walls
- Advanced Cellulose Research Studies
- Lignin and Wood Chemistry
- Polysaccharides Composition and Applications
- Biofuel production and bioconversion
- Crystallography and molecular interactions
- Plant nutrient uptake and metabolism
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Natural Fiber Reinforced Composites
- NMR spectroscopy and applications
- Solid-state spectroscopy and crystallography
- Advanced NMR Techniques and Applications
- Interconnection Networks and Systems
- Aerogels and thermal insulation
University of Warwick
2021-2025
University of Nottingham
2017
Abstract Hemicellulose polysaccharides influence assembly and properties of the plant primary cell wall (PCW), perhaps by interacting with cellulose to affect deposition bundling fibrils. However, functional differences between hemicelluloses such as glucomannan, xylan, xyloglucan (XyG) remain unclear. As most abundant hemicellulose, XyG is considered important in eudicot PCWs, but plants devoid show relatively mild phenotypes. We report here that a patterned β-galactoglucomannan (β-GGM)...
Water is one of the principal constituents by mass living plant cell walls. However, its role and interactions with secondary wall polysaccharides impact dehydration subsequent rehydration on molecular architecture are still to be elucidated. This work combines multidimensional solid-state 13C magic-angle-spinning (MAS) nuclear magnetic resonance (NMR) dynamics modeling decipher water in softwood The proximities between all main polymers, their conformations, interaction energies compared...
Abstract Plant biomass plays an increasingly important role in the circular bioeconomy, replacing non-renewable fossil resources. Genetic engineering of this lignocellulosic could benefit biorefinery transformation chains by lowering economic and technological barriers to industrial processing. However, previous efforts have mostly targeted major constituents woody biomass: cellulose, hemicellulose lignin. Here we report wood structure through introduction callose, a polysaccharide novel...
Wood modification using low molecular weight thermosetting resins improves the biological durability and dimensional stability of wood while avoiding increasingly regulated biocides. During process, resin monomers diffuse from cell lumen to wall, occupying micropore spaces before in situ curing at 150 °C. This study investigated mechanism wall diffusion multiple scales, comparing two test groups where was either facilitated or restricted. Antiswelling efficiency tests demonstrated improved...
Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils form the complex polysaccharide architecture of cell wall. How changes structure influence its wall solubility or promote appropriate interaction with is poorly understood. Glucomannan backbones acquire variable patterns galactosyl substitutions, depending on developmental stage and species. Here, we show fine-tuning modification glucomannans achieved by differing...
<title>Abstract</title> The structure of native plant cellulose, despite its abundance and utility in industry, remains elusive. cellulose several species was studied using 2D solid-state Nuclear Magnetic Resonance (NMR) <sup>13</sup>C labelled plants. Six major glucose environments were resolved which are common to the poplar wood, spruce wood grasses. maintained isolated holo-cellulose nanofibrils, allowing more detailed characterisation. There just two within fibril core have same NMR...
Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils form the complex polysaccharide architecture of cell wall. Glucomannan backbones acquire variable patterns galactosyl substitutions, depending on developmental stage and species. How changes structure influence its wall solubility or promote appropriate interaction with is poorly understood. Here, we show fine-tuning modification glucomannans achieved by differing...
Abstract The structure of native plant cellulose, despite its abundance and utility in industry, remains elusive. cellulose several species was studied using 2D solid-state Nuclear Magnetic Resonance (NMR) 13 C labelled plants. Six major glucose environments were resolved which are common to the poplar wood, spruce wood grasses. maintained isolated holo-cellulose nanofibrils, allowing more detailed characterisation. There just two within fibril core have same NMR chemical shifts as tunicate...
Similar to cellulose synthases (CESAs), synthase–like D (CSLD) proteins synthesize β-1,4-glucan in plants. CSLDs are important for tip growth and cytokinesis, but it was unknown whether they form membrane complexes vivo or produce microfibrillar cellulose. We produced viable CESA-deficient mutants of the moss Physcomitrium patens investigate CSLD function without interfering CESA activity. Microscopy spectroscopy showed that microfibrils indistinguishable from those vascular Correspondingly,...
Abstract The functional differences between plant cell wall hemicelluloses such as glucomannan, xylan and xyloglucan (XyG) remain unclear. These polysaccharides influence assembly properties of the wall, perhaps by interacting with cellulose to affect deposition bundling fibrils. As most abundant hemicellulose, XyG is considered important in eudicot primary walls (PCWs), but plants devoid show relatively mild phenotypes. We report here that a patterned β-galactoglucomannan (β-GGM) widespread...