A5027008402- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Polysaccharides Composition and Applications
- Advanced Cellulose Research Studies
- Plant Pathogenic Bacteria Studies
- Food composition and properties
- RNA Interference and Gene Delivery
- Banana Cultivation and Research
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Lignin and Wood Chemistry
- Force Microscopy Techniques and Applications
- Pancreatic function and diabetes
- Structural Analysis of Composite Materials
- Medieval Architecture and Archaeology
- Phytase and its Applications
- Diatoms and Algae Research
- Near-Field Optical Microscopy
- Plant Virus Research Studies
- Agricultural and Food Sciences
University of Sheffield
2016-2025
University of Leeds
2017-2023
Stomatal opening and closure depends on changes in turgor pressure acting within guard cells to alter cell shape [1]. The extent of these is limited by the mechanical properties cells, which will be largely dependent structure walls. Although it has long been observed that are anisotropic due differential thickening orientation cellulose microfibrils [2], our understanding composition wall allows them undergo repeated swelling deflation remains surprisingly poor. Here, we show walls rich...
The properties of (1,3)-β-glucans (i.e., callose) remain largely unknown despite their importance in plant development and defence. Here we use mixtures (1,3)-β-glucan cellulose, ionic liquid solution hydrogels, as proxies to understand the physico-mechanical callose. We show that after callose addition stiffness cellulose hydrogels is reduced at a greater extent than predicted from ideal mixing rule weighted average individual components' properties). In contrast, yield behaviour elastic...
Stomata are formed by a pair of guard cells which have thickened, elastic cell walls to withstand the large increases in turgor pressure that be generated open pore they surround. We characterized FOCL1, cell-expressed, secreted protein with homology Hyp-rich wall proteins. FOCL1-GFP localizes outer cuticular ledge and plants lacking FOCL1 produce stomata without ledge. Instead majority stomatal pores entirely covered over continuous fusion cuticle, consequently decreased levels...
Stomata regulate plant water use and photosynthesis by controlling leaf gas exchange. They do this reversibly opening the pore formed two adjacent guard cells, with limits of movement ultimately set mechanical properties cell walls surrounding epidermis.1Franks P.J. Farquhar G.D. The diversity stomata its significance in gas-exchange control.Plant Physiol. 2007; 143: 78-87https://doi.org/10.1104/pp.106.089367Crossref PubMed Scopus (368) Google Scholar,2Woolfenden H.C. Baillie A.L. Gray J.E....
Abstract Plant biomass plays an increasingly important role in the circular bioeconomy, replacing non-renewable fossil resources. Genetic engineering of this lignocellulosic could benefit biorefinery transformation chains by lowering economic and technological barriers to industrial processing. However, previous efforts have mostly targeted major constituents woody biomass: cellulose, hemicellulose lignin. Here we report wood structure through introduction callose, a polysaccharide novel...
Banana (Musa acuminata) and mango (Mangifera indica) are two of the most popular fruits eaten worldwide. They both soften during ripening but their textural attributes markedly different. This study aimed to elucidate molecular mechanism underpinning differences between banana mango. We used a novel combination methods at different scales analyse surface properties fruit cells potential contribution cell wall components oral processing texture perception. The results indicated that...
Abstract The plant hemicellulose xyloglucan (XyG) is secreted from the roots of numerous species, including cereals, and contributes towards soil aggregate formation in terrestrial systems. Whether XyG represents a key nutrient for plant-associated bacteria unclear. phylum Bacteroidota are abundant microbiome provide several beneficial functions their host. However, metabolic genomic traits underpinning success remain poorly understood. Here, using proteomics, bacterial genetics, genomics,...
The nature of the main primary mechanisms involved in lignocellulosic fast pyrolysis is often assumed to be radical mechanisms. Here we demonstrate that thermal depolymerization native hemicelluloses can undergo several and secondary concerted reactions leading light oxygenates compete with To model these at a microscopic level, used high-level quantum calculations based on functional theory. In parallel, set experimental data was collected confirm structural features extracted purified...
Summary A major route for cell-to-cell signaling is via cell wall-embedded pores termed plasmodesmata (PD) forming the symplasm. PD regulate many aspects of plant development and responses to environment however, our understanding what factors affect their structure permeability limited. In this paper, a meta-analysis presented as tool identification conditions affecting transport in silico generation proteomes species interest. The custom-built pipeline searches whole genome protein...
Abstract Cell walls underpin the mechanics of cell growth, intercellular signalling, and defence against pathogenic organisms. β-(1,3)-glucans (also known as callose) are polysaccharides found in plants, fungi, some bacterial species. In developing plant organs, callose accumulates around channels (plasmodesmata) controlling cell-to-cell communication. We developed monoclonal antibodies for detection using these identified distinct populations differing size secondary structure. Callose...
<title>Abstract</title> Pectins are enriched in primary cell walls and the middle lamella, determining cell-to-cell adhesion. O-acetylation of pectin backbones influences their physicochemical properties plays a role plant development interactions with environment. Here we report isolation mapping two trichome birefringence (tbr) mutants from an ozone-sensitivity screen show that TBR is required for leaf epidermal We further demonstrate RG-I rhamnose O-acetyltransferase acidic pH optimum,...