A5047571061- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Spectroscopy and Quantum Chemical Studies
- Microbial Community Ecology and Physiology
- Ovarian cancer diagnosis and treatment
- Microbial Metabolic Engineering and Bioproduction
- Trace Elements in Health
- Salmonella and Campylobacter epidemiology
- Endometriosis Research and Treatment
- Mitochondrial Function and Pathology
- Marine and coastal ecosystems
- ATP Synthase and ATPases Research
- Microbial Fuel Cells and Bioremediation
- Cervical Cancer and HPV Research
- Antioxidant Activity and Oxidative Stress
- Enzyme Structure and Function
- Endometrial and Cervical Cancer Treatments
- Bacterial Genetics and Biotechnology
- Metal Extraction and Bioleaching
- Plant and animal studies
- Lymphoma Diagnosis and Treatment
- Protein Structure and Dynamics
- Hydrocarbon exploration and reservoir analysis
- Advanced Electron Microscopy Techniques and Applications
University of Sheffield
2016-2025
University of California, San Francisco
2023
University of Southampton
1997-2018
National Oceanography Centre
2015-2018
Imperial College London
2017-2018
University of Exeter
2009
Royal Derby Hospital
2005
Derbyshire County Council
2005
Daresbury Laboratory
2003
Southampton General Hospital
1993-1998
Deep-learning methods have revolutionized protein structure prediction and design but are presently limited to protein-only systems. We describe RoseTTAFold All-Atom (RFAA), which combines a residue-based representation of amino acids DNA bases with an atomic all other groups model assemblies that contain proteins, nucleic acids, small molecules, metals, covalent modifications, given their sequences chemical structures. By fine-tuning on denoising tasks, we developed RFdiffusion...
Abstract Although AlphaFold2 (AF2) and RoseTTAFold (RF) have transformed structural biology by enabling high-accuracy protein structure modeling, they are unable to model covalent modifications or interactions with small molecules other non-protein that can play key roles in biological function. Here, we describe All-Atom (RFAA), a deep network capable of modeling full assemblies containing proteins, nucleic acids, molecules, metals, given the sequences polymers atomic bonded geometry...
Summary. Seventy‐five women admitted for sterilization were laparoscoped during menstruation. Retrograde spillage of menstrual blood was observed in 76%. The occurrence dysmenorrhoea not associated with blood, endometriosis or tubal pathology. Mild stage I detected 43% all the but only 54% those who had retrograde spill. spill seen 97% endometriosis. A significant association (P<0.00l) found between and damage due to implication these findings are discussed.
The regeneration of bioavailable phosphate from immobilized organophosphorus represents a key process in the global phosphorus cycle and is facilitated by enzymes known as phosphatases. Most bacteria possess at least one three phosphatases with broad substrate specificity, PhoA, PhoX, PhoD, whose activity optimal under alkaline conditions. production these repressed availability. Therefore, they are only fully functional when experience phosphorus-limiting growth Here, we reveal previously...
High-resolution structures of reaction-center light-harvesting 1 complexes provide new insights into quinone dynamics.
Chlorophototrophic organisms have a charge-separating reaction centre (RC) complex that receives energy from dedicated light-harvesting (LH) antenna. In the purple phototrophic bacteria, these two functions are embodied by 'core' photosynthetic component, RC-LH1 complex. complexes sit within membrane bilayer, with central RC wholly or partly surrounded curved array of LH1 subunits bind series bacteriochlorophyll (BChl) and carotenoid pigments. Decades research shown absorption light...
Fixing CO2 via photosynthesis requires ATP and NADPH, which can be generated through linear electron transfer (LET). However, depending on the environmental conditions, additional may required to fix CO2, by cyclic (CET). How balance between LET CET is determined remains largely unknown. Ferredoxin-NADP+ reductase (FNR) act as switch CET, channelling photosynthetic electrons when it bound photosystem I (PSI) or cytochrome b6f. The essential role of FNR in precludes use a direct gene...
Biophotovoltaic devices employ photosynthetic organisms at the anode of a microbial fuel cell to generate electrical power. Although range cyanobacteria and algae have been shown photocurrent in multitude architectures, mechanistic understanding extracellular electron transfer by phototrophs remains minimal. Here we describe mediatorless bioelectrochemical device measure electrogenic output planktonically grown cyanobacterium, Synechocystis sp. PCC6803. Light dependent production current is...
Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study fully exploit these organisms, the limited repertoire genetic tools parts must be expanded. A small number inducible promoters been used in cyanobacteria, allowing dynamic external control gene expression through addition specific inducer molecules. However, date suffer from various drawbacks including toxicity inducers, leaky absence photolability, latter...
Photosystem I (PSI) is the dominant photosystem in cyanobacteria and it plays a pivotal role cyanobacterial metabolism. Despite its biological importance, native organization of PSI thylakoid membranes poorly understood. Here, we use atomic force microscopy (AFM) to show that ordered, extensive macromolecular arrays complexes are present thylakoids from
Polyploidy is a well-described trait in some prokaryotic organisms; however, it unusual marine microbes from oligotrophic environments, which typically display tendency towards genome streamlining. The biogeochemically significant diazotrophic cyanobacterium Trichodesmium potential exception. With relatively large and comparatively high proportion of non-protein-coding DNA, appears to allocate more resources genetic material than closely related organisms within the same environment. Through...
Summary Cyanobacteria are prokaryotic phototrophs that, in addition to being excellent model organisms for studying photosynthesis, have tremendous potential light‐driven synthetic biology and biotechnology. These versatile resilient microorganisms harness the energy of sunlight oxidise water, generating chemical (ATP) reductant (NADPH) that can be used drive sustainable synthesis high‐value natural products genetically modified strains. In this commentary article Synthetic Microbiology...
Reaction centre light-harvesting 1 (RC–LH1) complexes are the essential components of bacterial photosynthesis. The membrane-intrinsic LH1 complex absorbs light and energy migrates to an enclosed RC where a succession electron proton transfers conserves as quinol, which is exported cytochrome bc1 complex. In some RC–LH1 variants quinols can diffuse through small pores in fully circular, 16-subunit ring, while others missing subunits create gap for quinol export. We used cryogenic microscopy...
Abstract Inorganic phosphate is the major bioavailable form of essential nutrient phosphorus. However, concentration in most natural habitats low enough to limit microbial growth. Under phosphate-depleted conditions some bacteria utilise phosphite and hypophosphite as alternative sources phosphorus, but molecular basis reduced phosphorus acquisition from environment not fully understood. Here, we present crystal structures ligand binding affinities periplasmic proteins bacterial ATP-binding...
Summary Species belonging to the filamentous cyanobacterial genus T richodesmium are responsible for a significant fraction of oceanic nitrogen fixation. The availability phosphorus ( P ) likely constrains growth in certain regions ocean. Moreover, species have recently been shown play role an emerging redox cycle, further highlighting key these microbes many biogeochemical processes contemporary Here, we show that erythraeum IMS101 can grow on reduced inorganic compound phosphite as its...
Biophotovoltaic devices utilize photosynthetic organisms such as the model cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis) to generate current for power or hydrogen production from light. These have been improved by both architecture engineering and genetic of phototrophic organism. However, approaches are limited lack understanding cellular mechanisms electron transfer internal metabolism cell exterior. Type IV pili implicated in extracellular transport (EET) some species...
SUMMARY In this Perspective article, we describe the visions of PhotoRedesign consortium funded by European Research Council how to enhance photosynthesis. The light reactions photosynthesis in individual phototrophic species use only a fraction solar spectrum, and high intensities can impair even damage process. consequence, expanding spectrum enhancing overall energy capacity process, while developing resilience stresses imposed intensities, could have strong positive impact on food...
Cytochrome bc 1 complexes are ubiquinol:cytochrome c oxidoreductases, and as such, they centrally important components of respiratory photosynthetic electron transfer chains in many species bacteria mitochondria. The minimal complex has three catalytic components, which cytochrome b , the Rieske iron–sulfur subunit, but function mitochondrial is modified by up to eight supernumerary subunits. from purple phototrophic bacterium Rhodobacter sphaeroides a single subunit called IV, absent...
Summary Methylmenaquinol : fumarate reductase (Mfr) is a newly recognized type of present in some ε‐proteobacteria, where the active site subunit (MfrA) localized periplasm, but for which physiological role has not been identified. We show that Campylobacter jejuni mfrABE operon transcribed from single promoter, with mfrA gene preceded by small open reading‐frame ( mfrX ) encoding C. ‐specific polypeptide unknown function. The growth characteristics and enzyme activities mutants menaquinol A...