A5012201986- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Advanced Electron Microscopy Techniques and Applications
- ATP Synthase and ATPases Research
- Force Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Enzyme Structure and Function
- Algal biology and biofuel production
- Mitochondrial Function and Pathology
- Antioxidant Activity and Oxidative Stress
University of Sheffield
2018-2024
Small, diffusible redox proteins play an essential role in electron transfer (ET) respiration and photosynthesis, sustaining life on Earth by shuttling electrons between membrane-bound complexes via finely tuned reversible interactions. Ensemble kinetic studies show transient ET form two distinct stages: "encounter" complex largely mediated electrostatic interactions, which subsequently, through subtle reorganization of the binding interface, forms a "productive" stabilized additional...
Abstract The reversible docking of small, diffusible redox proteins onto a membrane protein complex is common feature bacterial, mitochondrial and photosynthetic electron transfer (ET) chains. Spectroscopic studies ensembles such partners have been used to determine ET rates dissociation constants. Here, we report single-molecule analysis the forces that stabilise transient complexes. We examined interaction two components bacterial photosynthesis, cytochrome c2 reaction centre (RC) complex,...