A5059744923- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Bacteriophages and microbial interactions
- Plant Reproductive Biology
- Glycosylation and Glycoproteins Research
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Heat shock proteins research
- Protein Structure and Dynamics
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Enzyme Structure and Function
- Viral gastroenteritis research and epidemiology
- Blood properties and coagulation
- Polysaccharides Composition and Applications
- Hepatitis B Virus Studies
- Tendon Structure and Treatment
- Endoplasmic Reticulum Stress and Disease
- Light effects on plants
- Tissue Engineering and Regenerative Medicine
- Galectins and Cancer Biology
- Antibiotic Resistance in Bacteria
University of Leeds
2016-2025
Institute of Structural and Molecular Biology
2009-2024
Smith College
2017
Syracuse University
2017
University of Memphis
2017
Texas A&M University
2017
Louisiana State University Health Sciences Center New Orleans
2017
John Wiley & Sons (United States)
2017
Creative Research Enterprises (United States)
2016
University of Oxford
2016
Molecular recognition reagents are key tools for understanding biological processes and used universally by scientists to study protein expression, localisation interactions. Antibodies remain the most widely of such many show excellent performance, although some poorly characterised or have stability batch variability issues, supporting use alternative binding proteins as complementary applications. Here we report on Affimer research reagents. We selected 12 diverse molecular targets...
The Arabidopsis Co-expression Tool, ACT, ranks the genes across a large microarray dataset according to how closely their expression follows of query gene. A database stores pre-calculated co-expression results for approximately 21,800 based on data from over 300 arrays. These can be corroborated by calculation user-defined sub-sets arrays or experiments NASC/GARNet array dataset. Clique Finder (CF) identifies groups which are consistently co-expressed with each other list. parameters...
The network structure of the plant circadian clock is complex and direct regulatory interactions between individual components have proven particularly difficult to predict from genetic analyses. Here, we systematically investigate in vivo binding morning-specific transcription factor, LATE ELONGATED HYPOCOTYL (LHY) promoters other network. We then demonstrate functionality these by testing responsiveness target gene an ethanol-induced change expression level LHY protein. uncover novel,...
Using cross-linking coupled to matrix-assisted laser desorption/ionization mass spectrometry and CLIP-Seq sequencing, we determined the peptide oligonucleotide sequences at interfaces between capsid proteins genomic RNA of bacteriophage MS2. The results suggest that same coat protein (CP)–RNA maturation (MP)–RNA are used in every viral particle. portions contact with CP subunits span genome, consistent a large number discrete similar contacts within each Many these sites match previous...
Human islet amyloid polypeptide (hIAPP) self-assembles into fibrils which deposit in pancreatic islets of type 2 diabetes (T2D) patients. Here, we applied chemical kinetics to study the mechanism assembly wild-type hIAPP and its more amyloidogenic natural variant S20G. We show that aggregation both peptides involves primary nucleation, secondary nucleation elongation. also report discovery two structurally distinct small-molecule modulators assembly, one delaying wt hIAPP, but not S20G;...
Abstract Immune surveillance involves the continual migration of antigen-scavenging immune cells from tissues to downstream lymph nodes via lymphatic vessels. To enable such passage, first dock with entry receptor LYVE-1 on outer surface endothelium, using their endogenous hyaluronan glycocalyx, anchored by a second receptor, CD44. Why process should require two different receptors and which specific mechanism • interaction enables is however unknown. Here we describe crystal structures...
Summary The herbicide isoxaben is a highly specific and potent inhibitor of cellulose synthesis in plants. Nevertheless, suspension‐cultured cells can be habituated to grow high concentrations isoxaben, apparently compensate for the disruption by modulation other cell wall components. We have Arabidopsis characterized cellular genetic consequences. Near whole‐genome transcript profiling implicated novel genes assembly extended our understanding activity known wall‐related including...
In vitro analyses of plant GATA transcription factors have implicated some proteins in light-mediated and circadian-regulated gene expression, and, more recently, the analysis mutants has uncovered further diverse roles for factors. To facilitate function discovery 29 genes Arabidopsis (Arabidopsis thaliana), we experimentally verified structures determined expression patterns all family members across adult tissues suspension cell cultures, as well response to light signals from circadian...
Staphylococcus aureus and epidermidis form communities (called biofilms) on inserted medical devices, leading to infections that affect many millions of patients worldwide cause substantial morbidity mortality. As biofilms are resistant antibiotics, device removal is often required resolve the infection. Thus, there a need for new therapeutic strategies molecular data might assist their development. Surface proteins S. surface protein G (SasG) accumulation-associated (S. epidermidis) promote...
Successful development of plants resistant to salinity stress is problematic as a result the complex polygenic natures salt tolerance. Previously, alkaline extracts brown seaweed Ascophyllum nodosum have shown promise in enhancing plant tolerance toward abiotic stresses. To understand underlying molecular mechanisms, whole genome transcriptome Arabidopsis undergoing was analyzed by microarray analysis after treatment with chemical components A. (ANE). Treatment ANE induced many positive...
Abstract Recent evidence suggests that the ion channel TRPA1 is implicated in lung adenocarcinoma (LUAD), where its role and mechanism of action remain unknown. We have previously established membrane receptor FGFR2 drives LUAD progression through aberrant protein–protein interactions mediated via C-terminal proline-rich motif. Here we report N-terminal ankyrin repeats directly bind to motif inducing constitutive activation receptor, thereby prompting metastasis. Furthermore, show upon...
Summary We present a new WWW‐based tool for plant gene analysis, the Arabidopsis Co‐Expression Tool ( act ), based on large thaliana microarray data set obtained from Nottingham Stock Centre. The co‐expression analysis allows users to identify genes whose expression patterns are correlated across selected experiments or complete set. Results accompanied by estimates of statistical significance correlation relationships, expressed as probability P ) and expectation E values. Additionally,...
Plant cell walls are complex, multi-macromolecular assemblies of glycans and other molecules their compositions molecular architectures vary extensively. Even though the chemistry cell-wall is now well understood, it remains a challenge to understand diversity glycan configurations interactions in muro, how these relate changes biological mechanical properties walls. Here we describe detail method called epitope detection chromatography analysis matrix sub-populations inter-connections. The...
Phosphorus is an essential macronutrient for plant growth and deficient in ∼50% of agricultural soils. The transcription factor phosphate starvation response 1 (PHR1) plays a central role regulating the expression subset starvation-induced (PSI) genes through binding to cis-acting DNA element termed P1BS (PHR1-binding sequences). In Arabidopsis rice, activity AtPHR1/OsPHR2 regulated part by their downstream target SPX (Syg1, Pho81, Xpr1) proteins protein–protein interaction. Here, we provide...
Using RNA-coat protein crosslinking we have shown that the principal RNA recognition surface on interior of infectious MS2 virions overlaps with known peptides bind high affinity translational operator, TR, within phage genome. The data also reveal sequences genomic fragments in contact coat shell. These show remarkable overlap previous predictions based hypothesis virion assembly is mediated by multiple sequences-specific contacts at sites termed Packaging Signals (PSs). PSs are variations...
GPVI (glycoprotein VI) is a key molecular player in collagen-induced platelet signaling and aggregation. Recent evidence indicates that it also plays important role aggregation thrombus growth through interaction with fibrin(ogen). However, there are discrepancies the literature regarding whether monomeric or dimeric form of binds to fibrinogen at high affinity. The mechanisms not clear, including which region responsible for binding. We aimed gain further understanding level identify...
The elucidation of networks from a compendium gene expression data is one the goals systems biology and can be valuable source new hypotheses for experimental researchers. For Arabidopsis, there exist several thousand microarrays which form resource to learn.A novel Bayesian network-based algorithm infer regulatory introduced applied learn parts transcriptomic network in Arabidopsis thaliana large number (thousands) separate microarray experiments. Starting an initial set genes interest,...
Summary Plant cell walls are complex configurations of polysaccharides that fulfil a diversity roles during plant growth and development. They also provide sets biomaterials widely exploited in food, fibre fuel applications. The pectic polysaccharides, which comprise approximately third primary walls, form supramolecular structures with distinct glycan domains. Rhamnogalacturonan I ( RG –I) is highly structurally heterogeneous branched domain within the supramolecule contains...
The adaptor protein, Speckle-type BTB/POZ protein (SPOP), recruits substrates to the cullin-3-subclass of E3 ligase for selective ubiquitylation. Myddosome Myeloid differentiation primary response 88 (MyD88), is ubiquitylated by SPOP-based negatively regulate immune signaling, however, sequence rules SPOP-mediated substrate engagement and degradation are not fully understood. Here, we show that MyD88 interacts with SPOP through a long degron contains established SPOP-binding consensus an...