A5049760871- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Microbial infections and disease research
- Fibroblast Growth Factor Research
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Polysaccharides and Plant Cell Walls
- Streptococcal Infections and Treatments
- Acoustic Wave Resonator Technologies
- Plant Virus Research Studies
- Nanoparticle-Based Drug Delivery
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Nanopore and Nanochannel Transport Studies
- Connective tissue disorders research
- Polymer Surface Interaction Studies
- Polyamine Metabolism and Applications
- Surfactants and Colloidal Systems
- Bone Tissue Engineering Materials
- Microfluidic and Capillary Electrophoresis Applications
- RNA Interference and Gene Delivery
- Microfluidic and Bio-sensing Technologies
- Silk-based biomaterials and applications
University of Oklahoma Health Sciences Center
2014-2025
Caisson Laboratories (United States)
2012
University of Oklahoma
1998-2012
Rensselaer Polytechnic Institute
2008
University of Oxford
2005
University of Nebraska–Lincoln
1997
The University of Texas Medical Branch at Galveston
1993-1995
University of California, Irvine
1987-1990
Harvard University
1985
Hahnemann University Hospital
1982
ABSTRACT The hasA gene from Streptococcus equisimilis , which encodes the enzyme hyaluronan synthase, has been expressed in Bacillus subtilis resulting production of hyaluronic acid (HA) 1-MDa range. Artificial operons were assembled and tested, all contain along with one or more genes encoding enzymes involved synthesis UDP-precursor sugars that are required for HA synthesis. It was determined UDP-glucuronic is limiting B. overexpressing endogenous tuaD sufficient high-level HA. In...
The hyaluronan (HA) synthase of Group A Streptococci has been identified by transposon mutagenesis and deletion analysis. genes for the HA a recently UDP-Glc dehydrogenase (Dougherty, B. A., van de Rijn, I. (1993) J. Biol. Chem. 268, 7118-7124) reside on contiguous stretch 3.2-kilobase pair DNA that can direct biosynthesis in Enterococcus faecalis Escherichia coli as well mutant Streptococcus (DeAngelis, P. L., Papaconstantinou, J., Weigel, H. 14568-14571). contains 395 residues (calculated...
Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity heparan sulfate-mediated activities demands library structurally defined oligosaccharides. Chemical synthesis large oligosaccharides remains challenging. We report with different sulfation patterns and sizes from disaccharide building block using glycosyltransferases, C5-epimerase, sulfotransferases. This method offers generic approach to prepare possessing predictable structures.
Hyaluronan (or hyaluronic acid, HA) is a ubiquitous molecule that plays critical roles in numerous physiological functions vivo, including tissue hydration, inflammation, and joint lubrication. Both the abundance size distribution of HA biological fluids are recognized as robust indicators various pathologies disease progressions. However, such analyses remain challenging because conventional methods not sufficiently sensitive, have limited dynamic range, and/or only semi-quantitative. Here...
Abstract Immune surveillance involves the continual migration of antigen-scavenging immune cells from tissues to downstream lymph nodes via lymphatic vessels. To enable such passage, first dock with entry receptor LYVE-1 on outer surface endothelium, using their endogenous hyaluronan glycocalyx, anchored by a second receptor, CD44. Why process should require two different receptors and which specific mechanism • interaction enables is however unknown. Here we describe crystal structures...
Type A Pasteurella multocida, a prevalent animal pathogen, employs hyaluronan [HA] polysaccharide capsule to avoid host defenses. We utilized transposon insertional mutagenesis identify the P. multocida HA synthase, enzyme that polymerizes HA. DNA fragment from wild-type genomic library could direct production in vivo Escherichia coli, bacterium normally does not produce Analysis of truncated plasmids derived original clone indicated an open reading frame encoding 972-residue protein was...
A contiguous 3-kilobase pair region of DNA was isolated from Group Streptococcus pyogenes (GAS) that can direct hyaluronic acid (HA) capsule biosynthesis in acapsular mutants as well heterologous bacteria.The identified by transposon 916 insertional mutagenesis and subcloned into a plasmid shuttle vector.Mutant GAS or Enterococcus faecalis containing this plasmid, but not vector alone, displayed mucoid phenotype on agar plates, possessed seen light microscopy, produced HA quantities...
The length of the hyaluronan (HA) polysaccharide chain dictates its biological effects in many cellular and tissue systems. Long short HA polymers often appear to have antagonistic or inverse effects. However, no source very defined, uniform with sizes greater than 10 kDa is currently available. We present a method produce synthetic narrow size distributions range approximately 16 2 MDa. Pasteurella synthase enzyme, pmHAS, catalyzes synthesis polymer utilizing monosaccharides from UDP-sugar...
Sequence analysis of the 330-kilobase genome virus PBCV-1 that infects a chlorella-like green algae revealed an open reading frame, A98R, with similarity to several hyaluronan synthases. Hyaluronan is essential polysaccharide found in higher animals as well few pathogenic bacteria. Expression A98R gene product Escherichia coli indicated recombinant protein authentic synthase. expressed early infection and produced infected algae. These results demonstrate can encode enzyme capable...
Nanoparticle modification with poly(ethylene glycol) (PEG) is a widely used surface engineering strategy in nanomedicine. However, since the artificial PEG polymer may adversely impact nanomedicine safety and efficacy, alternative modifications are needed. Here, we explored "self" polysaccharide heparosan (HEP) to prepare colloidally stable HEP-coated nanoparticles, including gold silver nanoparticles liposomes. We found that HEP-coating reduced nanoparticle protein corona formation as...
The glycosaminoglycan hyaluronan (HA) plays important roles in diverse physiological functions where the distribution of its molecular weight (MW) can influence behavior and is known to change response disease conditions. During inflammation, HA undergoes a covalent modification which heavy chain subunits inter-alpha-inhibitor family proteins are transferred structure, forming chain-HA (HC•HA) complexes. While limited assessments HC•HA have been performed previously, determining size...
Type APasteurella multocida, an animal pathogen, employs a hyaluronan [HA] capsule to avoid host defenses. PmHAS, the 972-residue membrane-associated synthase, catalyzes transfer of both GlcNAc and GlcUA form HA polymer. To define catalytic domains, pmHAS mutants were analyzed. PmHAS1-703 is soluble, active synthase suggesting that carboxyl-terminus involved in membrane association native enzyme. PmHAS1-650 inactive as but retains GlcNAc-transferase activity. Within sequence, there...
Hyaluronan (HA), a long linear polymer composed of alternating glucuronic acid and N-acetylglucosamine residues, is an essential polysaccharide in vertebrates putative virulence factor certain microbes. All known HA synthases utilize UDP-sugar precursors. Previous reports describing the synthase enzymes from Streptococcus bacteria mammals, however, did not agree on molecular directionality elongation. We show here that synthase, PmHAS, Gram-negative P. multocida polymerizes chain by addition...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImmunochemical Confirmation of the Primary Structure Streptococcal Hyaluronan Synthase and Synthesis High Molecular Weight Product by Recombinant EnzymePaul L. DeAngelis Paul H. WeigelCite this: Biochemistry 1994, 33, 31, 9033–9039Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August 1994https://pubs.acs.org/doi/10.1021/bi00197a001https://doi.org/10.1021/bi00197a001research-articleACS...