A5069724772- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Seaweed-derived Bioactive Compounds
- Fibroblast Growth Factor Research
- Polysaccharides and Plant Cell Walls
- Heparin-Induced Thrombocytopenia and Thrombosis
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Supercapacitor Materials and Fabrication
- Microbial Metabolic Engineering and Bioproduction
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Fungal Biology and Applications
- Polysaccharides Composition and Applications
- Alzheimer's disease research and treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Advancements in Battery Materials
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Electrospun Nanofibers in Biomedical Applications
Rensselaer Polytechnic Institute
2016-2025
Southwest University
2023-2024
State Key Laboratory of Silkworm Genomic Biology
2023-2024
Guangdong Technion-Israel Institute of Technology
2020-2024
Guangzhou Institute of Energy Conversion
2024
First Affiliated Hospital of Anhui Medical University
2023
Anhui Medical University
2023
The Dainippon Silk Foundation
2023
Health Commission of Anhui Province
2023
Michigan State University
2022
Unique among fibroblast growth factors (FGFs), FGF19, -21, and -23 act in an endocrine fashion to regulate energy, bile acid, glucose, lipid, phosphate, vitamin D homeostasis. These FGFs require the presence of Klotho/betaKlotho their target tissues. Here, we present crystal structures FGF19 alone FGF23 complex with sucrose octasulfate, a disaccharide chemically related heparin. The conformation heparin-binding region between beta strands 10 12 diverges completely from common adopted by...
The conservation of positively charged residues in the N terminus hepatitis C virus (HCV) envelope glycoprotein E2 suggests an interaction viral with cell surface glycosaminoglycans. Using recombinant and virus-like particles as ligands for cellular binding, we demonstrate that heparan sulfate proteoglycans (HSPG) play important role mediating HCV envelope-target interaction. Heparin liver-derived highly sulfated but not other soluble glycosaminoglycans inhibited binding entry a...
Significance One important synthetic chemistry reaction endowed by nature is the decarboxylative carbon condensation using malonyl-CoA as donor. Previous metabolic engineering efforts centered on malonyl-CoA–dependent pathway have resulted in production of many value-added compounds. Here we mimicked native biological systems and used a dynamic regulatory network to optimize titers yield. The naturally existing transcriptional regulator FapR was rewired dynamically control gene expressions...
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, late 2019 and its subsequent spread to the rest world has created a pandemic situation unprecedented modern history. While ACE2 been identified as viral receptor, cellular polysaccharides have also implicated virus entry.
Lung cancer is the most common and aggressive tumor in world. Long non-coding RNA small nucleolar host gene 1 (lncRNA SNHG1) play critical roles progression of cancers. However, function underlying mechanism remain unclear lung cancer. In current study, we found that expression SNHG1 was up-regulated non-small cell (NSCLC) tissues lines. NSCLC patients with high were significantly correlated larger size, advanced TNM stage, lymph node metastasis poor overall survival than low expression....
Intravenous fluids, an essential component of sepsis resuscitation, may paradoxically worsen outcomes by exacerbating endothelial injury. Preclinical models suggest that fluid resuscitation degrades the glycocalyx, a heparan sulfate-enriched structure necessary for vascular homeostasis. We hypothesized glycocalyx degradation is associated with volume intravenous fluids administered during early resuscitation.We used mass spectrometry to measure plasma sulfate (a highly sensitive and specific...
As the leading anode material for sodium-ion batteries (SIBs), hard carbon (HC) still faces puzzle of low initial Coulombic efficiency (ICE) in achieving commercialization. From perspective precursors, ICE has been attributed to large specific surface area and porosity produced by rapid decomposition polymers during carbonization. Therefore, increasing cross-linking degree precursors will be an effective shortcut improve ICE. Herein, a facile pre-oxidation tactic was successfully employed...
Fibroblast growth factor (FGF)-23 has emerged as an endocrine regulator of phosphate and vitamin D metabolism. It is produced in bone and, unlike other FGFs, circulates the bloodstream to ultimately regulate handling production kidney. Presently, it unknown which seven principal FGF receptors (FGFRs) transmits FGF23 biological activity. Furthermore, molecular basis for mode action unclear. Herein, we performed surface plasmon resonance mitogenesis experiments comprehensively characterize...
Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in mid-hindbrain region during development. Although only difference between these isoforms is presence an additional 11 amino acids at N terminus possess remarkably different abilities to pattern midbrain anterior hindbrain. To reveal structural basis by which alternative splicing modulates organizing activity FGF8, we solved crystal structure FGF8b complex with "c" isoform FGF receptor 2 (FGFR2c). Using surface...
The prototypical fibroblast growth factor receptor (FGFR) extracellular domain consists of three Ig domains (D1–D3) which the two membrane-proximal D2 and D3 interconnecting D2–D3 linker bear determinants ligand binding specificity. In contrast, D1 D1–D2 are thought to play autoinhibitory roles in FGFR regulation. Here, we report crystal structure three-Ig form FGFR3c complex with FGF1, an FGF that binds promiscuously each seven principal FGFRs. this structure, completely disordered,...
The current working model for fibroblast growth factor receptor (FGFR) dimerization and activation requires the assembly of a ternary complex (FGF), FGFR, heparin or heparan sulfate proteoglycan (HSPG) on plasma membrane. recent FGF2−FGFR1−heparin crystal structure provides detailed but static view FGF−FGFR−heparin complex. However, kinetics has yet to be investigated. Here, we characterize FGF2, FGFR1, interactions using surface plasmon resonance (SPR). Binding constants binary FGF2/FGFR1...
Cellular binding and entry of hepatitis C virus (HCV) are the first steps viral infection represent a major target for antiviral antibodies novel therapeutic strategies. We have recently demonstrated that heparan sulfate (HS) plays key role in HCV envelope glycoprotein E2 to cells (Barth et al., J. Biol. Chem. 278:41003-41012, 2003). In this study, we characterized HCV-HS interaction analyzed its inhibition by host immune responses. Using recombinant glycoproteins, virus-like particles,...
The sulfated polysaccharide carrageenan (CGN) induces activation of NFkappaB and interleukin 8 (IL-8) in human colonic epithelial cells through a pathway innate immunity mediated by Bcl10 (B-cell CLL/lymphoma 10). In this report, we identify Toll-like receptor 4 (TLR4), member the family immune receptors, as surface membrane for CGN cells. Experiments with fluorescence-tagged demonstrated marked reduction binding to intestinal RAW 264.7 mouse macrophages, following exposure TLR4 blocking...
Degradation of the endothelial glycocalyx, a glycosaminoglycan (GAG)-rich layer lining vascular lumen, is associated with onset kidney injury in animal models critical illness. It unclear if similar pathogenic degradation occurs critically ill patients.To determine urinary indices GAG fragmentation are outcomes patients illnesses such as septic shock or acute respiratory distress syndrome (ARDS).We prospectively collected urine from 30 within 24 hours admission to Denver Health Medical...
Malonyl-CoA is the rate-limiting precursor involved in chain elongation reaction of a range value-added pharmaceuticals and biofuels. Development malonyl-CoA responsive sensors holds great promise overcoming critical pathway limitations optimizing production titers yields. By incorporating Bacillus subtilis trans-regulatory protein FapR cis-regulatory element fapO, we constructed hybrid promoter–regulatory system that responds to broad intracellular concentrations (from 0.1 1.1 nmol/mgDW)...