A5058526143- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Protein Structure and Dynamics
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Synthesis of β-Lactam Compounds
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Cytomegalovirus and herpesvirus research
- Signaling Pathways in Disease
- Metal complexes synthesis and properties
- Cancer Research and Treatments
- Neuropeptides and Animal Physiology
- Glutathione Transferases and Polymorphisms
- Viral Infections and Immunology Research
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Enzyme Production and Characterization
Institut de Biologia Molecular de Barcelona
2013-2023
Institute for Research in Biomedicine
2013-2023
Consejo Superior de Investigaciones Científicas
2003-2023
Chalmers University of Technology
2014
Vitenparken
2012
Unidades Centrales Científico-Técnicas
2011
Lomonosov Moscow State University
2011
University of Göttingen
2010
Max Planck Institute for Biophysical Chemistry
2010
University of Miami
2010
The crystal structures of d(CGCA3T3GCG) complex to the antitumor drug distamycin and DNA fragment alone were solved by x-ray diffraction at 2.2 2.5 A resolution, respectively. lies in narrow minor groove near center B-DNA covering 5 6 A.T base pairs. It is bound hydrogen bonding, van der Waals, electrostatic interactions. In addition, was found have an unusual conformation (dA)3.(dT)3 regions. These pairs a high positive propeller twist so that major adenine amino group located intermediate...
Three point turn: A metallosupramolecular helicate with trigonal-antiprismatic geometry fits perfectly into the central hydrophobic cavity of a three-way DNA junction allowing new mode recognition.
The molecular structure of the complex between a minor groove binding drug (netropsin) and DNA dodecamer d(CGCGATATCGCG) has been solved refined by single-crystal X-ray diffraction analysis to final R factor 20.0% 2.4-A resolution. crystal is similar that other related dodecamers with unit cell dimensions = 25.48 A, b 41.26 c 66.88 A in space group P2(1)2(1)2(1). In complex, netropsin binds central ATAT tetranucleotide segment narrow B-DNA double helix as expected. However, structural...
Hoechst dye 33258 is a planar drug molecule that binds to the minor groove of DNA, especially where there are number A.T base pairs. We have solved structure bound DNA dodecamer d(CGCGATATCGCG) at 2.3 A. This compared same with minor-groove-binding netropsin it, as well structures been for this containing central four pairs sequence AATT. find position in quite different from found other since it has an opposite orientation two structures. The covers three and extends its piperazine ring...
Abstract Double-stranded DNA bacteriophages package their genome at high pressure inside a procapsid through the portal, an oligomeric ring protein located unique capsid vertex. Once has been packaged, tail components assemble on portal to render mature infective virion. The tightly seals ejection conduit until infection, when its interaction with host membrane triggers opening of channel and viral is delivered cell. Using high-resolution cryo-electron microscopy X-ray crystallography, here...
Ellipticine is a natural plant product that has been found to be powerful anticancer drug. Although still unclear, its mechanism of action considered mainly based on DNA intercalation and/or the inhibition topoisomerase II. Many experimental data suggest an stacking interactions along major base-pair axis, but alternative binding modes have proposed, in particular for ellipticine derivatives. The 1.5 Å resolution structure complexed 6 bp oligonucleotide unveils mode and enables detailed...
During viral replication, herpesviruses package their DNA into the procapsid by means of terminase protein complex. In human cytomegalovirus (herpesvirus 5), is composed subunits UL89 and UL56. cleaves long concatemers unit-length genomes appropriate length for encapsidation. We used ESPRIT, a high-throughput screening method, to identify soluble purifiable fragment from library 18,432 randomly truncated ul89 constructs. The purified was crystallized its three-dimensional structure solved....