A5071391432- Plant Molecular Biology Research
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Computational Drug Discovery Methods
- Medieval Literature and History
- Ruminant Nutrition and Digestive Physiology
- Plant-Microbe Interactions and Immunity
- Plant and Biological Electrophysiology Studies
- Plant Parasitism and Resistance
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- RNA Research and Splicing
- Smart Agriculture and AI
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Nitrogen and Sulfur Effects on Brassica
- Polysaccharides and Plant Cell Walls
- Spectroscopy and Chemometric Analyses
- Plant responses to water stress
- Pasture and Agricultural Systems
John Innes Centre
2016-2025
Norwich Research Park
2016-2025
North Carolina Museum of Natural Sciences
2018-2024
Agriculture and Food
2016
University of Bristol
2012
Wellcome Trust
2003-2007
European Bioinformatics Institute
2003-2007
University of Arizona
2007
Lishman Health Foundation
2005
University of Liverpool
1997-2005
Significance Colonization of land by plants was a critical event for the emergence extant ecosystems. The innovations that allowed algal ancestor to succeed in such transition remain unknown. Beneficial interaction with symbiotic fungi has been proposed as one these innovations. Here we show genes required this appeared stepwise manner: Some evolved before colonization and others first plants. We thus propose preadapted beneficial employed gene networks colonize successfully.
Plants exhibit rapid, systemic signaling systems that allow them to coordinate physiological and developmental responses throughout the plant body, even highly localized quickly changing environmental stresses. The propagation of these signals is thought include processes ranging from electrical hydraulic networks waves reactive oxygen species (ROS) cytoplasmic Ca(2+) traveling plant. For wave system, involvement vacuolar ion channel TWO PORE CHANNEL1 (TPC1) has been reported. However,...
Calcium signals the making of symbiosis Plant cell nuclei respond to from symbiotic nitrogenfixing rhizobial bacteria or arbuscular mycorrhizal fungi with oscillating Ca 2+ release. Charpentier et al. identified a trio responsible channels in legume. These contain nuclear localization and are expressed root envelopes. The function early establishment produce oscillations release stores. Science , this issue p. 1102
Abstract Establishment of arbuscular mycorrhizal interactions involves plant recognition diffusible signals from the fungus, including lipochitooligosaccharides (LCOs) and chitooligosaccharides (COs). Nitrogen-fixing rhizobial bacteria that associate with leguminous plants also signal to their hosts via LCOs, so-called Nod factors. Here, we have assessed induction symbiotic signaling by (Myc) fungal-produced LCOs COs in legumes rice (Oryza sativa). We show Myc-LCOs tetra-acetyl chitotetraose...
Understanding how the cell uses a limited set of proteins to transduce very different signals into specific cellular responses is central goal biology and signal transduction disciplines. Although multifunctionality in widespread, mechanisms that allow differential modes signaling multifunctional pathways are not well defined. In legume plants, common symbiosis pathway composed at least seven mediates infection by both mycorrhizal fungi rhizobial bacteria. Here we show legumes differentially...
Nuclear-associated oscillations in calcium act as a secondary messenger the symbiotic signaling pathway of legumes. These are decoded by nuclear-localized and calmodulin-dependent protein kinase, activation which is sufficient to drive downstream responses. This implies that within nucleus predominant signals for legume symbiosis. However, mechanisms allow targeted release nuclear region have not been defined. Here we show symbiosis-induced changes occur both nucleoplasm perinuclear...
Abstract The accurate identification of ligand binding sites in protein structures can be valuable determining function. Once the site is known, it becomes easier to perform silico and experimental procedures that may allow type function determined. For example, pocket shape analysis relies heavily on correct localization site. We have developed SURFNET‐ConSurf, a modular, two‐stage method for identifying location potential pockets structures. In first stage, SURFNET program identifies...
Algorithms underlying the automatic model-building functionality of ARP/wARP software suite are presented. Finding most likely set Cα atoms from a given is formulated as constrained integer programming problem. The objective function density-weighted score for match between observed and expected chain conformation. Graph-search algorithms presented that find solutions to this problem in an efficient manner.
Abstract During flowering, primordia on the flanks of shoot apical meristem are specified to form flowers instead leaves. Like many plants, Arabidopsis thaliana integrates environmental and endogenous signals control timing reproduction. To study underlying regulatory logic floral transition, we used a combination modeling experiments define core gene network. We show that FLOWERING LOCUS T (FT) TERMINAL FLOWER1 (TFL1) act through FD PARALOG regulate transition. The major identity LEAFY...
Motivation: An increasing number of protein structures are being determined for which no biochemical characterization is available. The analysis structure and function assignment becoming an unexpected challenge a major bottleneck towards the goal well-annotated genomes. As shape plays crucial role in biomolecular recognition function, examination development description comparison techniques likely to be prime importance understanding structure–function relationships. Results: A novel...
Abstract The establishment of symbiotic associations in plants requires calcium oscillations that must be decoded to invoke downstream developmental programs. In animal systems, comparable are by calmodulin (CaM)–dependent protein kinases, but signaling involves a calcium/CaM–dependent kinase (CCaMK) is unique plants. CCaMK differs from the CaM kinases its dual ability bind free calcium, via binding EF-hand domains on protein, or complexed with CaM, domain. this study, we dissect regulation...
Ca2+ play a key role in cell signaling across organisms. The question of how simple ion can mediate specific outcomes has spurred research into the signatures and their encoding decoding machinery. Such studies have frequently focused on alone our understanding is integrated with other responses poor. Using vivo imaging different genetically encoded fluorescent sensors Arabidopsis (Arabidopsis thaliana) cells, we show that transients do not occur isolation but are accompanied by pH changes...
Recently, a large population of mRNA was shown to be able travel between plant organs via sieve elements as putative long-distance signaling molecule. However, mechanistic basis by which transcripts are selected for transport has not yet been identified. Here, we show that experimental mobility data in Arabidopsis can explained transcript abundance and half-life. This suggests the majority identified mobile accounted non-sequence-specific movement from companion cells into elements.
In plants, a variety of stimuli trigger long-range calcium signals that travel rapidly along the vasculature to distal tissues via poorly understood mechanisms. Here, we use quantitative imaging and analysis demonstrate traveling waves are mediated by diffusion bulk flow amino acid chemical messengers. We propose wounding triggers release acids diffuse locally through apoplast, activating calcium-permeable channel GLUTAMATE RECEPTOR-LIKE 3.3 as they pass. Over long distances vasculature,...
The design of a new versatile control system that will underlie future releases the automated model-building package ARP/wARP is presented. A sophisticated expert under development transform from very useful aid to truly capable delivering complete, well refined and validated models comparable in quality result intensive manual checking, rebuilding, hypothesis testing, refinement validation cycles an experienced crystallographer. In addition presentation this system, recent advances, ideas...
New procedures are outlined that enable ARP/wARP to automatically build protein models with diffraction data extending about 2.5 A. An overview of ongoing research is given and possible future advances discussed.
The first person in the British Isles to suffer from verified cholera was Sunderland, and he died on 26 October 1831.Thereafter there a country-wide epidemic lasting into 1832, then three more, 1848 1849, 1853 1854, 1866.The medical aspects of these events are interesting enough, especially gradual accumulation evidence favouring water-borne infection, but equal importance fascination is society's reaction onslaught this disease, newly arrived Britain.Dr. Morris surveys response stress...
Objective This study investigates the effects of Organophosphate (OP) pesticides exposure on cognitive and behavioral functioning Hispanic children living in an agricultural community. Methods Forty-eight were administered a battery measures, their parents teachers completed behavior rating scales. Children provided urine sample for analysis OP metabolites. Results All had detectable level at least one pesticide metabolite. Higher metabolite concentration levels significantly correlated with...
Summary Plants that form root‐nodule symbioses are within a monophyletic ‘nitrogen‐fixing’ clade and associated signalling processes shared with the arbuscular mycorrhizal symbiosis. Central to symbiotic nuclear‐associated oscillations in calcium ions (Ca 2+ ), occurring root hairs of several legume species response rhizobial Nod factor signal. In this study we expanded analysed for activation Ca oscillations, including non‐leguminous nitrogen‐fixing clade. We showed common feature legumes...