A5018072675- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Mycorrhizal Fungi and Plant Interactions
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Agronomic Practices and Intercropping Systems
- Bacteriophages and microbial interactions
- Polyamine Metabolism and Applications
- Themes in Literature Analysis
- Microbial Metabolic Engineering and Bioproduction
- Polymer-Based Agricultural Enhancements
- Microbial Community Ecology and Physiology
- Cancer-related Molecular Pathways
- Craniofacial Disorders and Treatments
- Light effects on plants
- Cancer Cells and Metastasis
- Plant and animal studies
- Soil Carbon and Nitrogen Dynamics
Stowers Institute for Medical Research
2025
Norwich Research Park
2011-2024
University of East Anglia
2016-2024
John Innes Centre
2011-2019
Inventors in the field of mechanical and electronic engineering can access multitudes components and, thanks to standardization, parts from different manufacturers be used combination with each other. The introduction BioBrick standards for assembly characterized DNA sequences was a landmark microbial engineering, shaping synthetic biology. Here, we describe standard Type IIS restriction endonuclease-mediated assembly, defining common syntax 12 fusion sites enable facile eukaryotic...
Abstract Establishment of arbuscular mycorrhizal interactions involves plant recognition diffusible signals from the fungus, including lipochitooligosaccharides (LCOs) and chitooligosaccharides (COs). Nitrogen-fixing rhizobial bacteria that associate with leguminous plants also signal to their hosts via LCOs, so-called Nod factors. Here, we have assessed induction symbiotic signaling by (Myc) fungal-produced LCOs COs in legumes rice (Oryza sativa). We show Myc-LCOs tetra-acetyl chitotetraose...
Abstract The establishment of symbiotic associations in plants requires calcium oscillations that must be decoded to invoke downstream developmental programs. In animal systems, comparable are by calmodulin (CaM)–dependent protein kinases, but signaling involves a calcium/CaM–dependent kinase (CCaMK) is unique plants. CCaMK differs from the CaM kinases its dual ability bind free calcium, via binding EF-hand domains on protein, or complexed with CaM, domain. this study, we dissect regulation...
Abstract Nucleotide composition is suggested to infer gene functionality and ecological adaptation of species distinct environments. However, the underlying biological function nucleotide dictating environmental adaptations largely unknown. Here, we systematically analyze transcriptomes across 1000 plants (1KP) their corresponding habitats. Intriguingly, find that growing in cold climates have guanine (G)-enriched transcriptomes, which are prone forming RNA G-quadruplex structures. Both...
Dimethylsulfoniopropionate (DMSP) is an important marine anti-stress compound, with key roles in global nutrient cycling, chemotaxis and, potentially, climate regulation. Recently, diverse Actinobacteria, α- and γ-proteobacteria were shown to initiate DMSP synthesis via the methionine (Met) S-methyltransferase enzyme (MmtN), generating S-methyl-Met (SMM). Here we characterize a roseobacterial MmtN, providing structural mechanistic insights into this enzyme. We propose that MmtN uses...
Plant synthetic biology and cereal engineering depend on the controlled expression of transgenes interest. Most in plant species to date has relied heavily use a few, well-established constitutive promoters achieve high levels expression; however, transgene can also be influenced by codon optimization, intron-mediated enhancement varying terminator sequences. these alternative approaches for regulating have only been tested small-scale experiments, typically testing single gene It is...
Craniofacial microsomia (CFM) is a genetically and phenotypically heterogeneous disorder characterized by hypoplasia of facial tissue that often asymmetric. Affected tissues typically include the ears (external internal), mandible, maxilla, but various extracranial anomalies have also been reported. Loss-of-function variants in SF3B2 gene recently reported 8 cases CFM, representing one more common genetic causes identified to date. To better define full phenotypic spectrum associated with ,...
Calcium and Ca(2+)/calmodulin-dependent protein kinase (CCaMK) plays a critical role in the signaling pathway that establishes root nodule symbiosis arbuscular mycorrhizal symbiosis. Calcium-dependent autophosphorylation is central to regulation of CCaMK, this has been shown promote calmodulin binding. Here, we report regulatory mechanism Medicago truncatula CCaMK (MtCCaMK) through S344 calmodulin-binding/autoinhibitory domain. The phospho-ablative mutation S344A did not have significant...
Summary Calcium signalling is central to many plant processes, with families of calcium decoder proteins having expanded across the green lineage and redundancy existing between decoders. The liverwort Marchantia polymorpha has fast become a new model plant, but decoders that exist in this species remain unclear. We performed phylogenetic analyses identify calcineurin B‐like (CBL) CBL‐interacting protein kinase (CIPK) network M. . analysed CBL‐CIPK expression during salt stress, determined...
Abstract Nucleotide composition is suggested to infer gene functionality and ecological adaptation of species distinct environments. However, the underlying biological function nucleotide dictating environmental adaptations largely unknown. Here, we systematically analyzed compositions transcriptomes across 1000 plants (1KP) their corresponding habitats. Intriguingly, found that growing in cold climates have guanine (G)-enriched transcriptomes, which are prone forming RNA G-quadruplex...
Abstract Honest communication between potential partners with conflicting interests is generally thought to require costly signals. Costly signalling can explain partner choice when it possible link a strategic cost an individual’s quality, like in mate choice. However, mutualisms, usually impossible the likelihood that will behave cooperatively future. In fact, signals Nod factors rhizobial bacteria, which form symbioses leguminous plants, are evidence of cost-free, honest situations...
The organosulfur compound dimethylsulfoniopropionate (DMSP) has key roles in stress protection, global carbon and sulfur cycling, chemotaxis, is a major source of climate-active gases. Saltmarshes are hotspots for DMSP cycling due to Spartina cordgrasses that produce exceptionally high concentrations DMSP. Here, anglica, we identify the plant genes underpin high-level synthesis: methionine S-methyltransferase (MMT), S-methylmethionine decarboxylase (SDC) DMSP-amine oxidase (DOX). Homologs...
ABSTRACT Mucus secreting goblet cells play a vital role in the maintenance of tissue homeostasis. Here we report discovery an enigmatic mechanism for generation calcium signals that couple cholinergic input to secretion hydrated mucus human colonic stem cell niche. Mechanistic insights this study were derived from native crypts and crypt-like organoids expressing MUC2-mNEON. Importantly, demonstrate niche is also niche, activation muscarinic receptors initiates at apical pole intestinal...
SUMMARY Calcium signalling is central to many plant processes, with families of calcium decoder proteins having expanded across the green lineage and redundancy existing between decoders. The liverwort Marchantia polymorpha has fast become a new model plant, but it unclear what decoders exist in this species. We have performed phylogenetic analyses identify Calcineurin B-Like (CBL) CBL-Interacting Protein Kinase (CIPK) network M. . analysed CBL-CIPK expression during salt stress, determined...