A5100736149- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Photosynthetic Processes and Mechanisms
- Methane Hydrates and Related Phenomena
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Protein Hydrolysis and Bioactive Peptides
- Algal biology and biofuel production
- Scheduling and Optimization Algorithms
- Seaweed-derived Bioactive Compounds
- Studies on Chitinases and Chitosanases
- Optimization and Search Problems
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Advanced Manufacturing and Logistics Optimization
- Marine and coastal ecosystems
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Wastewater Treatment and Nitrogen Removal
- Polyamine Metabolism and Applications
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Traditional Chinese Medicine Analysis
- Food Industry and Aquatic Biology
Ocean University of China
2018-2025
Shandong University
2016-2025
State Key Laboratory of Microbial Technology
2016-2025
Qingdao National Laboratory for Marine Science and Technology
2016-2025
ShenZhen People’s Hospital
2018-2025
Shenzhen University
2025
Biotechnology Research Center
2009-2025
North China University of Water Resources and Electric Power
2023-2024
Henan Province Water Conservancy Survey and Design Research
2023-2024
Qufu Normal University
2013-2024
ABSTRACT Genomic information has already been applied to prokaryotic species definition and classification. However, the contribution of genome sequence genus delimitation less studied. To gain insights into for prokaryotes, we attempted reveal genus-level genomic differences in current classification system delineate boundary a on basis information. The average nucleotide identity between two genomes can be used delineation, but it is not suitable demarcation. We percentage conserved...
Methane is supersaturated in surface seawater and shallow coastal waters dominate global ocean methane emissions to the atmosphere. Aerobic oxidation (MOx) can reduce atmospheric evasion, but magnitude control of MOx remain poorly understood. Here we investigate sources fates East China Sea map rates by training machine-learning models. We show produced during methylphosphonate decomposition under phosphate-limiting conditions sedimentary release also source methane. High observed these...
Thiosulfate oxidation by microbes has a major impact on global sulfur cycling. Here, we provide evidence that bacteria within various Roseobacter lineages are important for thiosulfate in marine biofilms. We isolate and sequence the genomes of 54 biofilm-associated strains, finding conserved sox gene clusters plasmids, pointing to niche-specific lifestyle. Analysis ocean metagenomic data suggests strains abundant biofilms mats substrates, including stones, artificial surfaces, plant roots,...
Antibiosis is one of the widespread strategies used by Trichoderma spp. against plant fungal pathogens, mechanism which, however, remains poorly understood. Peptaibols are a large family antimicrobial peptides produced Our previous study showed that trichokonins, type peptaibol from pseudokoningii SMF2, exhibited antibiotic activities pathogens. In this study, we first demonstrated trichokonin VI (TK VI) induced extensive apoptotic programmed cell death in For deeper insight into involved...
Nitrogen is one of the most important nutrients needed for plants and algae to survive, photosynthetic ability related nitrogen abundance. Red are unique eukaryotic organisms in evolution algae, as they contain phycobilisomes (PBSs) on their thylakoid membranes. In this report, vivo chlorophyll (Chl) a fluorescence kinetics nitrogen-starved Porphyridium cruentum were analyzed determine effects deficiency performance using multi-color pulse amplitude modulation (PAM) fluorometer. Due...
Trichoderma spp. are well-known biocontrol agents because of their antimicrobial activity against bacterial and fungal phytopathogens. However, the biochemical mechanism antiviral remains largely unknown. In this study, we found that Trichokonins, peptaibols isolated from pseudokoningii SMF2, could induce defense responses systemic resistance in tobacco (Nicotiana tabacum var. Samsun NN) mosaic virus (TMV) infection. Local Trichokonin (100 nM) treatment led to 54% lesion inhibition, 57%...
Severe fever with thrombocytopenia syndrome virus (SFTSV), which can cause hemorrhagic fever-like illness, is a newly discovered bunyavirus in China. The pathogenesis of SFTSV infection poorly understood. However, it has been suggested that immune mechanisms, including cytokines and chemokines, play an important role disease pathogenesis. In the present study, we investigated host cytokine chemokine profiles serum samples patients from Northeast China explored possible correlation between...
Oyster extracts have been reported to many bioactive peptides. But the function of oyster peptides produced by proteolysis is still unknown. In this study, oligopeptide-enriched hydrolysates from (Crassostrea gigas) were using protease Bacillus sp. SM98011 at laboratory level, and scaled up pilot (100 L) plant (1,000 levels with same conditions. And antitumor activity immunostimulating effects in BALB/c mice investigated. The growth transplantable sarcoma-S180 was obviously inhibited a...
Abstract Predator-prey interactions play important roles in the cycling of marine organic matter. Here we show that a Gram-negative bacterium isolated from sediments ( Pseudoalteromonas sp. strain CF6-2) can kill Gram-positive bacteria diverse peptidoglycan (PG) chemotypes by secreting metalloprotease pseudoalterin. Secretion enzyme requires Type II secretion system. Pseudoalterin binds to glycan strands Gram positive bacterial PG and degrades peptide chains, leading cell death. The released...
Abstract Although many kinds of exopolysaccharides (EPSs) from microorganisms have been used in industry, the exploration and utilization EPSs polar is still rather rare. In this study, a flavobacterial strain, SM1127, Arctic brown alga Laminaria , was screened for its high EPS production (2.11 g/l) identified as belonging to genus Polaribacter . The secreted by strain SM1127 has molecular mass 220 kDa it mainly comprises N -acetyl glucosamine, mannose glucuronic acid residues bound...
Alginate lyases that degrade alginate via a β-elimination reaction fall into seven polysaccharide lyase (PL) families. Although the structures and catalytic mechanisms of in other PL families have been clarified, those family PL6 yet to be revealed. Here, crystal structure AlyGC, from marine bacterium Glaciecola chathamensis S18K6T, was solved, its mechanism illustrated. AlyGC is homodimeric enzyme adopts distinct lyases. Each monomer contains N-terminal domain functionally unknown...
Abstract Background The Arctic and Antarctic are the two most geographically distant bioregions on earth. Recent sampling efforts following metagenomics have shed light global ocean microbial diversity function, yet microbiota of polar regions has not been included in such analyses. Results Here a metagenomic study seawater samples ( n = 60) collected from different depths at 28 locations zones was performed, together with metagenomes Tara Oceans. More than 7500 (19%) seawater-derived...
Abstract Printable and stretchable conductive elastomers have promising applications for epidermal wearable electronics, soft robotics, etc. However, these materials usually present poor performances in monitoring dynamic strains. The monitored signals are distorted lose key physical signs, which limit their practical applications. Through investigating kinetic behaviors of pathways along longitudinal transverse directions materials, the mechanism signal distortion under strains is...
Energy-autonomous wearable human activity monitoring is imperative for daily healthcare, benefiting from long-term sustainable uses. Herein, a fully self-powered system, enabling real-time and assessments of multimodal health parameters including knee joint movement, metabolic energy, locomotion speed, skin temperature, which are by highly-efficient flexible thermoelectric generators (f-TEGs) proposed developed. The system composed f-TEGs, fabric strain sensors, ultra-low-power edge...
Cryptophyte plastids originated from a red algal ancestor through secondary endosymbiosis. photosystem I (PSI) associates with transmembrane alloxanthin-chlorophyll a/c proteins (ACPIs) as light-harvesting complexes (LHCs). Here, we report the structure of photosynthetic PSI-ACPI supercomplex cryptophyte Chroomonas placoidea at 2.7-Å resolution obtained by crygenic electron microscopy. represents unique PSI-LHCI intermediate in evolution to diatom PSI-LHCI. The is composed monomeric PSI core...
Abstract Symbiodinium are the photosynthetic endosymbionts for corals and play a vital role in supplying their coral hosts with products, forming nutritional foundation high-yield reef ecosystems. Here, we determine cryo-electron microscopy structure of photosystem I (PSI) supercomplex PSI core composed 13 subunits including 2 previously unidentified subunits, PsaT PsaU, as well peridinin-Chl / c -binding light-harvesting antenna proteins (AcpPCIs). The PSI–AcpPCI exhibits distinctive...
Marine bacteria play important roles in the degradation and cycling of algal polysaccharides. However, dynamics epiphytic bacterial communities their polysaccharide during kelp decay are still unclear. Here, we performed metagenomic analyses to investigate identities predicted metabolic abilities early late stages
Prenylation modifications of natural products play essential roles in chemical diversity and bioactivities, but imidazole modification prenyltransferases are not well investigated. Here, we discover a dimethylallyl tryptophan synthase family prenyltransferase, AuraA, that catalyzes the rare dimethylallylation on moiety biosynthesis aurantiamine. Biochemical assays validate AuraA could accept both cyclo-(L-Val-L-His) cyclo-(L-Val-DH-His) as substrates, while prenylation modes completely...
Recently, a new class of glycosaminoglycan (GAG) lyases (GAGases) belonging to PL35 family has been discovered with an ultrabroad substrate spectrum that can degrade three types uronic acid-containing GAGs (hyaluronic acid, chondroitin sulfate and heparan sulfate) or even alginate. In this study, the structures GAGase II from Spirosoma fluviale VII Bacteroides intestinalis DSM 17393 were determined at 1.9 2.4 Å resolution, respectively, their catalytic mechanism was investigated by...