A5100405256- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Seaweed-derived Bioactive Compounds
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Biochemical and Structural Characterization
- Marine Biology and Environmental Chemistry
- Hydrocarbon exploration and reservoir analysis
- Nanoplatforms for cancer theranostics
- Probiotics and Fermented Foods
- Advanced Nanomaterials in Catalysis
- Peptidase Inhibition and Analysis
- Anaerobic Digestion and Biogas Production
- Microbial Metabolism and Applications
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Protein Hydrolysis and Bioactive Peptides
- Antibiotic Resistance in Bacteria
- Marine Biology and Ecology Research
- Neuroscience and Neuropharmacology Research
- Wastewater Treatment and Nitrogen Removal
- Petroleum Processing and Analysis
University of Hong Kong
2016-2025
Affiliated Hospital of Qingdao University
2020-2025
Qingdao University
2020-2025
Beijing Jiaotong University
2025
Sichuan University
2015-2024
Northwestern Polytechnical University
2024
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2020-2024
Mayo Clinic
2020-2024
HKU-Pasteur Research Pole
2024
Dingxi City People's Hospital
2024
Abstract With an ever-increasing amount of (meta)genomic data being deposited in sequence databases, (meta)genome mining for natural product biosynthetic pathways occupies a critical role the discovery novel pharmaceutical drugs, crop protection agents and biomaterials. The genes that encode these are often organised into gene clusters (BGCs). In 2015, we defined Minimum Information about Biosynthetic Gene cluster (MIBiG): standardised format describes minimally required information to...
Abstract Recent big data analyses have illuminated marine microbial diversity from a global perspective, focusing on planktonic microorganisms. Here, we analyze 2.5 terabases of newly sequenced datasets and the Tara Oceans metagenomes to study biofilm-forming We identify more than 7,300 ‘species’ that are undetected in seawater analyses, increasing known oceans by 20%, provide evidence for differentiation across oceanic niches. Generation gene distribution profile reveals functional core...
Thiosulfate oxidation by microbes has a major impact on global sulfur cycling. Here, we provide evidence that bacteria within various Roseobacter lineages are important for thiosulfate in marine biofilms. We isolate and sequence the genomes of 54 biofilm-associated strains, finding conserved sox gene clusters plasmids, pointing to niche-specific lifestyle. Analysis ocean metagenomic data suggests strains abundant biofilms mats substrates, including stones, artificial surfaces, plant roots,...
Abstract The worldwide prevalence of infections caused by antibiotic-resistant Gram-negative bacteria poses a serious threat to public health due the limited therapeutic alternatives. Cationic peptides represent large family antibiotics and have attracted interest their diverse chemical structures potential for combating drug-resistant pathogens. Here, we analyze 7395 bacterial genomes investigate capacity biosynthesis cationic nonribosomal with activity against bacteria. Applying this...
Abstract Bacilli are ubiquitous low G+C environmental Gram-positive bacteria that produce a wide assortment of specialized small molecules. Although their natural product biosynthetic potential is high, robust molecular tools to support the heterologous expression large gene clusters in Bacillus hosts rare. Herein we adapt transformation-associated recombination (TAR) yeast design single genomic capture and vector for antibiotic production subtilis . After validating this direct cloning...
N-methyl-D-aspartate (NMDA) receptors play critical roles in synaptic transmission and plasticity. Activation of NMDA by synaptically released L-glutamate also requires occupancy co-agonist binding sites the tetrameric receptor either glycine or D-serine. Although D-serine appears to be predominant at receptors, transport mechanisms involved homeostasis brain are poorly understood. In this work we show that SLC1 amino acid transporter family members SLC1A4 (ASCT1) SLC1A5 (ASCT2) mediate...
The geological characteristics and production practices of the major middle- high-maturity shale oil exploration areas in China are analyzed. Combined with laboratory results, it is clear that three essential conditions, i.e. economic initial production, commercial cumulative single well, large-scale recoverable reserves confirmed by testing determine whether continental can be put into development. quantity quality movable hydrocarbons to crucial development oil, focuses evaluation...
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a fascinating group of natural products that exhibit diverse structural features bioactivities. P450-catalyzed RiPPs stand out as unique but underexplored family. Herein, we introduce rule-based genome mining strategy harnesses the intrinsic biosynthetic principles RiPPs, including co-occurrence co-conservation precursors P450s interactions between them, successfully facilitating identification RiPPs. Intensive...
The major enrichment type of shale oil in the Chang 73 Upper Triassic Yanchang Formation Ordos Basin is unknown. This paper analyzes organic matter transformation ratio, hydrocarbon expulsion efficiency and roof/floor sealing conditions shale, evaluates this interval. average ratio about 45%; other words, more than 50% matters have not transformed to hydrocarbons, lower maturity, greater proportion untransformed matters. cumulative 27.5% on average, total plus retained hydrocarbons 70%....
Abstract Background Lactic acid bacteria (LAB) produce various bioactive secondary metabolites (SMs), which endow LAB with a protective role for the host. However, biosynthetic potentials of LAB-derived SMs remain elusive, particularly in their diversity, abundance, and distribution human microbiome. Thus, it is still unknown to what extent are involved microbiome homeostasis. Results Here, we systematically investigate potential from 31,977 genomes, identifying 130,051 metabolite gene...
Recently, much attention has been given to discovering natural compounds as potent anti-cancer candidates. In the present study, effects of fumigaclavine C, isolated from a marine-derived fungus, Aspergillus fumigatus, was evaluated in vitro. order investigate impact C on inhibition proliferation and induction apoptosis breast cancer, MCF-7 cells were treated with various concentrations showed significant cytotoxicity towards cells. Anti-proliferation analyzed via cell mobility...
Cuproptosis is a newly recognized copper-dependent nonapoptotic form of cell death, which stimulate studies exploring copper-based nanomaterials to treat cancer through distinct mechanistic action. However, it remains challenge completely eradicate tumors via monotherapy. Herein, copper-doped BiSex (CBS) nanozyme was developed boost αPD-L1-mediated immune checkpoint blocking (ICB) synergetic apoptosis/cuproptosis-induced immunogenic death (ICD). The defect-engineered CBS exhibits strong...
Abstract Antimicrobial resistance remains a significant global threat, driving up mortality rates worldwide. Ribosomally synthesized and post-translationally modified peptides have emerged as promising source of novel peptide antibiotics due to their diverse chemical structures. Here, we report the discovery new aminovinyl-(methyl)cysteine (Avi(Me)Cys)-containing through synergistic approach combining biosynthetic rule-based omics mining heterologous expression. We first bioinformatically...
Dyslipidemia may induce chronic kidney disease and trigger both ferroptosis endoplasmic reticulum (ER) stress, but the instigating factors are incompletely understood. We tested hypothesis that different models of dyslipidemia engage distinct injury mechanisms. Wild-type (WT) or proprotein-convertase subtilisin/kexin type-9 (PCSK9)-gain-of-function (GOF) Ossabaw pigs were fed with a 6-month normal diet (ND) high-fat (HFD) (n = 5-6 each). Renal function fat deposition studied in vivo using...