A5020146174- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Algal biology and biofuel production
- Marine and coastal plant biology
- Biocrusts and Microbial Ecology
- Plant Molecular Biology Research
- Protist diversity and phylogeny
- Lichen and fungal ecology
- Fungal Biology and Applications
- Photosynthetic Processes and Mechanisms
- Botany and Plant Ecology Studies
- Plant and animal studies
- Allergic Rhinitis and Sensitization
- Genomics and Phylogenetic Studies
- Mycorrhizal Fungi and Plant Interactions
- Toxin Mechanisms and Immunotoxins
- Microbial Community Ecology and Physiology
- Diatoms and Algae Research
- Silymarin and Mushroom Poisoning
- Plant Taxonomy and Phylogenetics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Polyamine Metabolism and Applications
- Biochemical and biochemical processes
- Plant and fungal interactions
- Fermentation and Sensory Analysis
Japan Women's University
2014-2024
Niigata University
2022-2024
Niigata Prefectural Central Hospital
2023
Material (Belgium)
2012
National Sagamihara Hospital
2011
The University of Tokyo
1997-2006
Chiba University
2005
Max Planck Institute for Plant Breeding Research
2005
National Institute for Basic Biology
2005
Japan Science and Technology Agency
2000-2003
Significance Colonization of land by plants was a critical event for the emergence extant ecosystems. The innovations that allowed algal ancestor to succeed in such transition remain unknown. Beneficial interaction with symbiotic fungi has been proposed as one these innovations. Here we show genes required this appeared stepwise manner: Some evolved before colonization and others first plants. We thus propose preadapted beneficial employed gene networks colonize successfully.
The MADS-box genes of land plants are extensively diverged to form a superfamily and important in various aspects development including the specification floral organs as homeotic selector genes. closest relatives freshwater green algae charophyceans. To study origin evolution plant genes, we characterized these three charophycean algae: stonewort Chara globularis , coleochaete Coleochaete scutata desmid Closterium peracerosum–strigosum–littorale complex. Phylogenetic analyses suggested that...
Summary Gibberellins (GAs) are essential for the development of fertile flowers in tomato, and may also be required immediately after fertilization. In GA‐biosynthetic pathway, reactions catalyzed by GA 20‐oxidases have been implicated as site regulation. To study regulation biosynthesis flower early fruit development, we isolated three tomato 20‐oxidase cDNA clones, Le20ox‐1, ‐2 ‐3 . The genes showed different organ‐specific patterns mRNA accumulation. Analysis transcript levels genes, well...
Abstract Evolutionary mechanisms underlying innovation of cell types have remained largely unclear. In multicellular eukaryotes, the evolutionary molecular origin sperm differentiation is unknown in most lineages. Here, we report that algal ancestors land plants, changes DNA-binding domain ancestor MYB transcription factor DUO1 enabled recognition a new cis- regulatory element. This event led to motile sperm. After neo-functionalization, acquired lineage-specific expression common plants....
Summary The Closterium peracerosum–strigosum–littorale complex (Closterium, Zygnematophyceae) has an isogamous mating system. Members of the Zygnematophyceae are closest relatives to extant land plants and distantly related chlorophytic models, for which a genetic basis type (MT) determination been reported. We thus investigated MT in Closterium. sequenced genomes representing two MTs, mt + −, identified CpMinus1 , gene linked mt− phenotype. analyzed its function using reverse genetics...
Using degenerate primers designed by deduced amino acid sequences of known aldehyde oxidases (AO) from maize and bovine, two independent cDNA fragments were amplified reverse transcription-polymerase chain reaction (PCR). The corresponding full-length cDNAs (atAO-1 atAO-2; 4,484 4,228 bp long, respectively) cloned screening the Arabidopsis library followed rapid amplification end-PCR. These are highly homologous at both nucleotide sequence levels, showed high similarity with those tomato...
Degenerate oligonucleotide primers based on the amino acid sequences of gibberellin (GA) 20‐oxidases from pumpkin and Arabidopsis were used in nested polymerase chain reactions to generate an internal cDNA fragment rice ( Oryza satiya L.) seedlings. Using this as a probe, full‐length (pOs20ox) was isolated library constructed The deduced sequence Os20ox showed good identity (42–55%) with GA . Recombinant protein, produced Escherichia coli , catalyzed conversion 12 53 9 20 respectively. In...
In cucumber (Cucumis sativus L.) plants, it has been reported that a high correlation existed between the evolution of ethylene from apices and development female flowers. We isolated three distinct cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase cucumber. Among these, only CS-ACS2 mRNA was detected at gynoecious in which flowers were developing. The expression gene examined cultivars (Rensei, Ougonmegami 2-gou Shimoshirazu) by RNA gel blot analysis. these cultivars, both...
Although charophycean algae form a relevant monophyly with embryophytes and hence occupy fundamental place in the development of Streptophyta, no tools for genetic transformation these organisms have been developed. Here we present first stable nuclear system unicellular Zygnematales, Closterium peracerosum–strigosum–littorale complex (C. psl complex), which is one most useful experimental research on algae. When vector, pSA106, containing dominant selectable marker ble...
Upon fertilization in angiosperms, one sperm cell fuses with the egg to produce a zygote, and, via karyogamy, parental genetic information is combined form diploid zygotic genome. Recently, analyses parentally imbalanced rice zygotes indicated that genomes are utilized synergistically different functions, and genes transcribed from paternal or maternal allele might play important roles development. Herein, we first conducted single nucleotide polymorphism-based mRNA-sequencing using...
The molecular bases for the evolution of male–female sexual dimorphism are possible to study in volvocine algae because they encompass entire range reproductive morphologies from isogamy oogamy. In 1978, Charlesworth suggested model a gamete size gene becoming linked sex-determining or mating type locus (MT) as mechanism anisogamy. Here, we carried out first comprehensive candidate MT-linked oogamy gene, MAT3/RB, across lineage. We found that anisogamy/oogamy predates extremely high...
Here, we cloned the CpRLK1 gene, which encodes a receptor-like protein kinase expressed during sexual reproduction, from heterothallic Closterium peracerosum–strigosum–littorale complex, one of closest unicellular alga to land plants. Mating-type plus (mt+) cells with knockdown showed reduced competence for reproduction and formed an abnormally enlarged conjugation papilla after pairing mt– cells. The were unable release naked gamete, is indispensable zygote formation. We suggest that...
Because the secondary plastids of Euglenophyta and Chlorarachniophyta are very similar to green plant in their pigment composition, it is generally considered that ancestral algae were engulfed by other eukaryotic host cells become these two algal divisions. Recent molecular phylogenetic studies have attempted resolve positions plastids; however, almost all analyzed only plastid‐encoded genes. This limitation may affect results comparisons between genes from primary plastids, because...
Summary The zygnematophycean algae occupy an important phylogenetic position as the closest living relatives of land plants. Reverse genetics is quite useful for dissecting functions genes. However, this strategy requires genetic transformation, and there are only a few reports successful transformation in algae. Here, we established simple highly efficient technique unicellular alga Closterium peracerosum‐strigosum‐littorale complex using square electric pulse‐generating electroporator...
A unicellular charophyte alga, Closterium peracerosum–strigosum–littorale complex (C. psl. complex), has been studied in order to obtain basic information regarding sexual reproduction plants. Systems for gene introduction and transient expression were developed endogenous genes using phleomycin resistance (ble) Chlamydomonas green fluorescent protein (cgfp) as selection markers. These have codon usage similar that of the C. complex. To drive these strongly into cells, two native promoters...
Abstract Heterothallic strains of the Closterium peracerosum-strigosum-littorale ( C . psl .) complex have two sexes, mating-type plus (mt + ) and minus − ). Conjugation between these sexes is regulated by sex pheromones, protoplast-release-inducing protein (PR-IP) PR-IP Inducer, which are produced mt cells, respectively. mediates release protoplasts from cells during mating. In this study, we examined mechanism action CpRLP1 receptor-like 1 ), was previously identified in a cDNA microarray...