A5069124324- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Enzyme-mediated dye degradation
- Glycosylation and Glycoproteins Research
- Advanced Cellulose Research Studies
- Carbohydrate Chemistry and Synthesis
- Enzyme Structure and Function
- Lignin and Wood Chemistry
- Food composition and properties
- Protein Structure and Dynamics
- Plant nutrient uptake and metabolism
- Biochemical and Molecular Research
- thermodynamics and calorimetric analyses
- ATP Synthase and ATPases Research
- Diamond and Carbon-based Materials Research
- Membrane Separation Technologies
- Plant-Microbe Interactions and Immunity
- Studies on Chitinases and Chitosanases
- Cell Adhesion Molecules Research
- Digestive system and related health
Novozymes (Denmark)
2014-2024
Technical University of Denmark
2004-2009
Missouri University of Science and Technology
2007
Ecologie Microbienne Lyon
2004
The enzymatic degradation of recalcitrant plant biomass is one the key industrial challenges 21st century. Accordingly, there a continuing drive to discover new routes promote polysaccharide degradation. Perhaps most promising approach involves application “cellulase-enhancing factors,” such as those from glycoside hydrolase (CAZy) GH61 family. Here we show that enzymes are unique family copper-dependent oxidases. We demonstrate copper needed for maximal activity and formation cellodextrin...
The Sabatier principle states that optimal catalysis occurs when interactions between catalyst and substrate are of intermediary strength. Although qualitative in nature, this concept has proven extremely useful within (nonbiochemical) heterogeneous catalysis. In the current work, we show may be applied to an interfacial enzyme reaction. Specifically, studied breakdown cellulose by different cellulases (wild types variants) found results could rationalized so-called volcano plots emblematic...
Abstract Hemicellulose polysaccharides influence assembly and properties of the plant primary cell wall (PCW), perhaps by interacting with cellulose to affect deposition bundling fibrils. However, functional differences between hemicelluloses such as glucomannan, xylan, xyloglucan (XyG) remain unclear. As most abundant hemicellulose, XyG is considered important in eudicot PCWs, but plants devoid show relatively mild phenotypes. We report here that a patterned β-galactoglucomannan (β-GGM)...
Cereal arabinoxylans (AXs) are complex polysaccharides in terms of their pattern arabinose and ferulic acid substitutions, which influence properties structural nutritional applications. We have evaluated the molecular structure three AXs from wheat rye with distinct substitutions on activity β-xylanases different glycosyl hydrolase families (GH 5_34, 8, 10 11). The accessibility xylanases, resulting specific profiles arabinoxylan-oligosaccharides (AXOS). GH10 xylanase Aspergillus aculeatus...
Xylanase A from the phytopathogenic bacterium Erwinia chrysanthemi is classified as a glycoside hydrolase family 30 enzyme (previously in 5) and specialized for degradation of glucuronoxylan. The recombinant was crystallized with aldotetraouronic acid β-D-xylopyranosyl-(1→4)-[4-O-methyl-α-D-glucuronosyl-(1→2)]-β-D-xylopyranosyl-(1→4)-D-xylose ligand. crystal structure enzyme-ligand complex solved at 1.39 Å resolution. ligand xylotriose moiety occupies subsites -1, -2 -3, whereas methyl...
The degradation of the plant cell wall by glycoside hydrolases is central to environmentally sustainable industries. major polysaccharides are cellulose and xylan, a highly decorated β-1,4-xylopyranose polymer. Glycoside displaying multiple catalytic functions may simplify enzymes required degrade walls, increasing industrial potential these composite structures. Here we test hypothesis that hydrolase family 43 (GH43) provides suitable scaffold for introducing additional into target complex...
Plant lignocellulosic biomass can be a source of fermentable sugars for the production second generation biofuels and biochemicals. The recalcitrance this plant material is one major obstacles in its conversion into sugars. Biomass primarily composed secondary cell walls, which made cellulose, hemicelluloses lignin. Xylan, hemicellulose, binds to cellulose microfibril hypothesised form an interface between lignin cellulose. Both softwood hardwood xylan carry glucuronic acid side branches. As...
Summary Polysaccharide structural complexity not only influences cell wall strength and extensibility but also hinders pathogenic biotechnological attempts to saccharify the wall. In certain species tissues, glucuronic acid side groups on xylan exhibit arabinopyranose or galactose decorations whose genetic evolutionary basis is completely unknown, impeding efforts understand their function engineer digestibility. Genetics polysaccharide profiling were used identify responsible loci in...
Lignin-carbohydrate complexes (LCCs) are believed to influence the recalcitrance of lignocellulosic plant material preventing optimal utilization biomass in e.g. forestry, feed and biofuel applications. The recently emerged carbohydrate esterase (CE) 15 family glucuronoyl esterases (GEs) has been proposed degrade ester LCC bonds between glucuronic acids xylans lignin alcohols thereby potentially improving delignification when applied conjunction with other cellulases, hemicellulases...
Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, extraordinary recalcitrance plant toward breakdown necessitates continued search enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides route functional discovery such in complex mixtures industrially relevant Here, we show detection identification β-xylosidases endo-β-1,4-xylanases secretomes Aspergillus...
Abstract Enzyme reactions, both in Nature and technical applications, commonly occur at the interface of immiscible phases. Nevertheless, stringent descriptions interfacial enzyme catalysis remain sparse, this is partly due to a shortage coherent experimental data guide assess such work. In work, we produced kinetically characterized 83 cellulases, which revealed conspicuous linear free energy relationship (LFER) between substrate binding strength activation barrier. The scaling occurred...
Abstract Heparan sulfate is a highly modified O -linked glycan that performs diverse physiological roles in animal tissues. Though quickly modified, it initially synthesised as polysaccharide of alternating β- d -glucuronosyl and N -acetyl-α- -glucosaminyl residues by exostosins. These enzymes generally possess two glycosyltransferase domains (GT47 GT64)—each thought to add one type monosaccharide unit the backbone. Although previous structures murine exostosin-like 2 (EXTL2) provide insight...
Abstract Background Spent coffee grounds (SCG) are the most abundant waste byproducts generated from beverage production worldwide. Typically, these seen as and end up in landfills. However, SCG contain valuable compounds that can be valorized used different applications. Notably, they rich carbohydrates, primarily galactomannan, arabinogalactan type II, cellulose. Within framework of a circular bioeconomy, targeted degradation polysaccharides via tailored cocktail carbohydrate-active...
Expression of a Trichoderma reesei gene coding for putative GH30 xylanase in Aspergillus oryzae led to isolation and purification novel exhibiting catalytic properties different from those the previously characterized XYN IV T. reesei. The enzyme, named VI, exhibited similar appendage-dependent glucuronoxylanases recognized only bacteria. VI showed high specific activity on xylans or xylooligosaccharides containing 4-O-methyl-D-glucuronic acid D-glucuronic side substituents. cleavage main...
Abstract Background Plant cell wall polysaccharide composition varies substantially between species, organs and genotypes. Knowledge of the structure these polysaccharides, accompanied by a suite well characterised glycosyl hydrolases will be important for success lignocellulosic biofuels. Current methods used to characterise enzymatically released plant oligosaccharides are relatively slow. Results A method software was developed allowing use DNA sequencer profile derived from...
The pulp and paper industry is one of the most important industrial sectors worldwide, has considerable potential for sustainable fractionation lignocellulosic biomass to provide valuable compounds. Ultrafiltration (UF) a suitable separation technique profitable production hemicelluloses from process water thermomechanical pulping (ThMP), but limited by membrane fouling. Improvements in cleaning protocols new alternative agents are required ensure long lifetime, thus process. This study,...
Fungal genomes often contain several copies of genes that encode carbohydrate active enzymes having similar activity. The usually have slight sequence variability, and it has been suggested the multigenecity represents distinct reaction optima versions enzyme. Whether represent differences in substrate attack proficiencies enzyme rarely considered. Aspergillus species pectin lyases (EC 4.2.2.10), which all belong to polysaccharide lyase subfamily PL1_4 CAZy database. differ terms identity...