A5034234340- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolism and Applications
- Microbial Natural Products and Biosynthesis
- Glycosylation and Glycoproteins Research
- Diet, Metabolism, and Disease
- Enzyme Structure and Function
- Polysaccharides and Plant Cell Walls
- Amino Acid Enzymes and Metabolism
- Enzyme-mediated dye degradation
- Digestive system and related health
- Luminescence and Fluorescent Materials
- Polyamine Metabolism and Applications
- Lysosomal Storage Disorders Research
- Molecular Sensors and Ion Detection
- Click Chemistry and Applications
- Polysaccharides Composition and Applications
- Organic Electronics and Photovoltaics
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Mobile Learning in Education
- Natural Antidiabetic Agents Studies
- Molecular Junctions and Nanostructures
University of York
2019-2024
University of British Columbia
2015-2018
Canada's Michael Smith Genome Sciences Centre
2015-2018
Okanagan University College
2018
Akron Children's Hospital
2016
Université de Montréal
2011-2012
Bombardier (Canada)
2011-2012
The carbohydrate-rich coating of human tissues and cells provide a first point contact for colonizing invading bacteria. Commensurate with N-glycosylation being an abundant form protein glycosylation that has critical functional roles in the host, some host-adapted bacteria possess machinery to process N-linked glycans. pathogen Streptococcus pneumoniae depolymerizes complex N-glycans enzymes sequentially trim N-glycan down Man3GlcNAc2 core prior release glycan from by...
The enzymatic conversion of plant biomass has been recently revolutionized by the discovery lytic polysaccharide monooxygenases (LPMO) that catalyze oxidative cleavage polysaccharides. These powerful enzymes are secreted a large number fungal saprotrophs and important components commercial enzyme cocktails used for industrial conversion. Among 33 AA9 LPMOs encoded genome Podospora anserina, PaLPMO9H catalyzes mixed C1/C4 cellulose cello-oligosaccharides. Activity on several hemicelluloses...
Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, extraordinary recalcitrance plant toward breakdown necessitates continued search enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides route functional discovery such in complex mixtures industrially relevant Here, we show detection identification β-xylosidases endo-β-1,4-xylanases secretomes Aspergillus...
The xyloglucan endotransglycosylase/hydrolase (XTH) gene family encodes enzymes of central importance to plant cell wall remodeling. evolutionary history XTH products is incompletely understood vis-à-vis the larger body bacterial endoglycanases in Glycoside Hydrolase Family 16 (GH16). To provide molecular insight into this issue, high-resolution X-ray crystal structures and detailed enzyme kinetics an extant transitional endoglucanase (EG) were determined. Functionally intermediate between...
Identifying and characterizing the enzymes responsible for an observed activity within a complex eukaryotic catabolic system remains one of most significant challenges in study biomass-degrading systems. The debranching both hemicellulosic pectinaceous polysaccharides requires production α-l-arabinofuranosidases among wide variety coexpressed carbohydrate-active enzymes. To selectively detect identify produced by fungi grown on biomass, potential covalent inhibitors probes which mimic...
Stable 2-aminobithiophenes were prepared using the Gewald reaction. The resulting push-pull bithiophenes exhibited both unprecedented high fluorescence yields and stability in addition to demonstrating on-off properties.
Abstract The recent discovery of zinc‐dependent retaining glycoside hydrolases (GHs), with active sites built around a Zn(Cys) 3 (Glu) coordination complex, has presented unresolved mechanistic questions. In particular, the proposed mechanism, depending on Zn‐coordinated cysteine nucleophile and passing through thioglycosyl enzyme intermediate, remains controversial. This is primarily due to expected stability intermediate C−S bond. To facilitate study this atypical we report synthesis...
Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection β-1,4-glucanase activity typically relies on monitoring the breakdown purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting activities which can be detected complicating tracing back to specific within complex As a tool for rapid identification β-1,4-glucanases, series glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan...
Bacteria and yeasts grow on biomass polysaccharides by expressing excreting a complex array of glycoside hydrolase (GH) enzymes. Identification annotation such GH pools, which are valuable commodities for sustainable energy chemistries, conventional means (genomics, proteomics) complicated, as primary sequence or secondary structure alignment with known active enzymes is not always predictive new ones. Here we report "low-tech", easy-to-use, sensitive multiplexing activity-based...
Fluorescent α,α′-diamide substituted bi- and terthiophene derivatives were prepared by Stille Suzuki couplings. Their one-pot deprotection coupling with 2-thiophene carboxaldehyde led to stable conjugated azomethines. These exhibited electrochromic properties, they used fabricate a working device.
The precise catalytic strategies used for the breakdown of complex bacterial polysaccharide xanthan, an increasingly frequent component processed human foodstuffs, have remained a mystery. Here, we present characterization endo-xanthanase from Paenibacillus nanensis. We show that it is CAZy family 9 glycoside hydrolase (GH9) responsible hydrolysis xanthan backbone capable generating tetrameric oligosaccharides lyase 8 (PL8) lyase-treated xanthan. Three-dimensional structure determination...
Fungal saccharification of lignocellulosic biomass occurs concurrently with the secretion a diverse collection proteins, together functioning as catalytic system to liberate soluble sugars from insoluble composite biomaterials. How different fungi respond substrates is fundamental interest developing industry. Among cornerstones fungal enzyme systems are highly expressed cellulases (endo-β-glucanases and cellobiohydrolases). Recently, cyclophellitol-derived activity-based probe (ABP-Cel) was...
α-L-Arabinofuranosidases from glycoside hydrolase family 51 use a stereochemically retaining hydrolytic mechanism to liberate nonreducing terminal α-L-arabinofuranose residues plant polysaccharides such as arabinoxylan and arabinan. To date, more than ten fungal GH51 α-L-arabinofuranosidases have been functionally characterized, yet no structure of enzyme has solved. In contrast, seven bacterial structures, with low sequence similarity the enzymes, determined. Here, crystallization...
An annotated reference genome has revealed P. putredinis NO1 as a useful resource for the identification of new lignocellulose-degrading enzymes biorefining woody plant biomass. Utilizing "structure-omics"-based searching strategy, we identified potentially lignocellulose-active sequences that would have been missed by traditional sequence methods. These identifications, alongside discovery novel enzymatic functions from this underexplored lineage with recent phenol oxidase cleaves main...
New cyclophellitol-derived probes covalently label β-mannosidases, enabling the detection of diverse mannosidases via activity-based protein profiling.
A series of water-soluble 1-amino-naphthalenes and 2-amino-fluorenes are prepared. These serve as model fluorophores for measuring the thermodynamics kinetics fluorescence quenching with phenylboronic acids aliphatic amines. Steady-state time-resolved investigated using Stern–Volmer method. Diffusion limited constants exergonic electron transfer derived 5-amino-1-napthol 2-aminofluorene derivatives acid and/or an imine. No endergonic or observed 5-sulfonamide, 5-sulfonic acid,...
GH127 and GH146 microorganismal retaining β-l-arabinofuranosidases, expressed by human gut microbiomes, feature an atypical catalytic domain unusual mechanism of action. We recently reported that both Bacteroides thetaiotaomicron BtGH146 Bifidobacterium longum HypBA1 are inhibited β-l-arabinofuranosyl cyclophellitol epoxide, supporting the action a zinc-coordinated cysteine as nucleophile, where in most GH families, aspartate or glutamate is employed. This work presents panel epoxides...
NO1 is a plant biomass-degrading ascomycete with propensity to target the most recalcitrant components of lignocellulose. Here we applied proteomics and activity-based protein profiling (ABPP) investigate ability
Abstract The recent discovery of zinc‐dependent retaining glycoside hydrolases (GHs), with active sites built around a Zn(Cys) 3 (Glu) coordination complex, has presented unresolved mechanistic questions. In particular, the proposed mechanism, depending on Zn‐coordinated cysteine nucleophile and passing through thioglycosyl enzyme intermediate, remains controversial. This is primarily due to expected stability intermediate C−S bond. To facilitate study this atypical we report synthesis...