A5047828675- Microbial bioremediation and biosurfactants
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Pesticide and Herbicide Environmental Studies
- Enzyme-mediated dye degradation
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Chemical synthesis and alkaloids
- Steroid Chemistry and Biochemistry
- Pharmacogenetics and Drug Metabolism
- Chemical Reactions and Isotopes
- Studies on Chitinases and Chitosanases
- Polyamine Metabolism and Applications
- Electrochemical sensors and biosensors
- Microbial Community Ecology and Physiology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Alkaloids: synthesis and pharmacology
- Toxic Organic Pollutants Impact
- Microfluidic and Capillary Electrophoresis Applications
- Wastewater Treatment and Nitrogen Removal
- Aldose Reductase and Taurine
University of York
2015-2024
Universidad Nacional Autónoma de México
2018
National Academies of Sciences, Engineering, and Medicine
2010
National Research Council Canada
2010
Biotechnology Research Institute
2010
University of Washington
2010
University of Cambridge
1997-2008
University of Edinburgh
2000-2008
Institute of Structural and Molecular Biology
2008
University of Glasgow
2007
Thermobia domestica belongs to an ancient group of insects and has a remarkable ability digest crystalline cellulose without microbial assistance. By investigating the digestive proteome Thermobia, we have identified over 20 members uncharacterized family lytic polysaccharide monooxygenases (LPMOs). We show that this LPMO spans across several clades Tree Life, is origin, was recruited by early arthropods with possible roles in remodeling endogenous chitin scaffolds during development...
We have studied the physiological and genetic responses of Arabidopsis thaliana L. (Arabidopsis) to gold. The root lengths seedlings grown on nutrient agar plates containing 100 mg/L gold were reduced by 75%. Oxidized was subsequently found in roots shoots these plants, but nanoparticles (reduced gold) only observed tissues. used a microarray-based study monitor expression candidate genes involved metal uptake transport upon exposure. There up-regulation plant stress response such as...
The oomycete Phytophthora infestans is a damaging crop pathogen and model organism to study plant-pathogen interactions. We report the discovery of family copper-dependent lytic polysaccharide monooxygenases (LPMOs) in plant pathogenic oomycetes its role infection by P. show that LPMO-encoding genes are up-regulated early during secreted enzymes oxidatively cleave backbone pectin, charged cell wall. crystal structure most abundant these LPMOs sheds light on ability recognize degrade...
A mixed microbial culture capable of metabolizing the explosive RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) was obtained from soil enrichments under aerobic and nitrogen-limiting conditions. bacterium, Stenotrophomonas maltophilia PB1, isolated used as a sole source nitrogen for growth. Three moles per mole RDX, yielding metabolite identified by mass spectroscopy 1H nuclear magnetic resonance analysis methylene-N-(hydroxymethyl)-hydroxylamine-N'-(hydroxymethyl)nitroamin e. The bacterium...
Enterobacter cloacae PB2 was originally isolated on the basis of its ability to utilize nitrate esters, such as pentaerythritol tetranitrate (PETN) and glycerol trinitrate, sole nitrogen source for growth. The enzyme responsible is an NADPH-dependent reductase designated PETN reductase. E. found be capable slow aerobic growth with 2,4,6-trinitrotoluene (TNT) source. Dinitrotoluenes were not produced could used sources. Purified reduce TNT hydride-Meisenheimer complex, which further reduced...
Several independent studies of bacterial degradation nitrate ester explosives have demonstrated the involvement flavin-dependent oxidoreductases related to old yellow enzyme (OYE) yeast. Some these enzymes also transform nitroaromatic explosive 2,4,6-trinitrotoluene (TNT). In this work, catalytic capabilities five members OYE family were compared, with a view correlating structure and function. The activity profiles differed substantially; no one compound proved be good substrate for all...
Plants, as predominantly sessile organisms, have evolved complex detoxification pathways to deal with a diverse range of toxic chemicals. The elasticity this stress response system additionally enables them tackle relatively recently produced, novel, synthetic pollutants. One such compound is the explosive 2,4,6-trinitrotoluene (TNT). Large areas soil and groundwater are contaminated TNT, which both highly recalcitrant degradation, persists in environment for decades. Although TNT...
The digestion of lignocellulose is attracting attention both in terms basic research into its metabolism by microorganisms and animals, also as a means converting plant biomass biofuels. Limnoriid wood borers are unusual because, unlike other wood-feeding they do not rely on symbiotic microbes to help digest lignocellulose. absence the digestive tract suggests that limnoriid produce all enzymes necessary for themselves. In this study we report analysis ESTs from system Limnoria...
Increasing global demand for animal protein combined with an urgent need to advance towards sustainability, as enshrined within the Sustainable Development Goals (SDGs), has spurred development of insects a source in and fish feed. The acceptance feasibility these advances are nevertheless poorly understood. This study aimed identify opportunities, challenges solutions black soldier fly larvae-based feed production, through stakeholder interviews Malaysia, analysed using thematic analysis...
Abstract Background Salt marshes are major natural repositories of sequestered organic carbon with high burial rates matter, produced by highly productive native flora. Accumulated predominantly exists as lignocellulose which is metabolised communities functionally diverse microbes. However, the organisms that orchestrate this process and enzymatic mechanisms employed regulate accumulation, composition permanence stock not yet known. We applied meta-exo-proteome proteomics 16S rRNA gene...
ABSTRACT Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a high explosive which presents an environmental hazard as major land and groundwater contaminant. Rhodococcus rhodochrous strain 11Y was isolated from contaminated capable of degrading RDX when provided the sole source nitrogen for growth. Products degradation in resting-cell incubations were analyzed found to include nitrite, formaldehyde, formate. No ammonium excreted into medium, no dead-end metabolites observed. The gene...
A mixed microbial culture capable of metabolizing the explosive pentaerythritol tetranitrate (PETN) was obtained from soil enrichments under aerobic and nitrogen-limiting conditions. strain Enterobacter cloacae, designated PB2, isolated this found to use PETN as a sole source nitrogen for growth. Growth yields suggested that 2 3 mol utilized per PETN. The metabolites dinitrate, 3-hydroxy-2,2-bis-[(nitrooxy)methyl]propanal, 2,2-bis-[(nitrooxy)methyl]-propanedial were identified by mass...
Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, strongly inhibited steroids, and capable of reducing the unsaturated bond 2-cyclohexen-1-one.
Widespread contamination of land and groundwater has resulted from the use, manufacture, storage military explosive hexa-hydro-1,3,5-trinitro-1,3,5-triazine (RDX). This led to a requirement for sustainable, low-cost method remediate this problem. Here, we present characterization an unusual microbial P450 system able degrade RDX, consisting flavodoxin reductase XplB fused flavodoxin-cytochrome XplA. The affinity XplA xenobiotic compound RDX is high ( K d = 58 μM) comparable with m other...
Nature uses a diversity of glycoside hydrolase (GH) enzymes to convert polysaccharides sugars. As lignocellulosic biomass deconstruction for biofuel production remains costly, natural GH offers starting point developing industrial enzymes, and fungal family 7 (GH7) cellobiohydrolases, in particular, provide significant hydrolytic potential mixtures. Recently, GH7 have been found other kingdoms life besides fungi, including animals protists. Here, we describe the vivo spatial expression...